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Literature summary extracted from

  • Fukamizo, T.; Hayashi, K.; Tamoi, M.; Fujimura, Y.; Kurotaki, H.; Kulminskaya, A.; Kitaoka, M.
    Enzymatic hydrolysis of 1,3-1,4-beta-glucosyl oligosaccharides by 1,3-1,4-beta-glucanase from Synechocystis PCC6803: a comparison with assays using polymer and chromophoric oligosaccharide substrates (2008), Arch. Biochem. Biophys., 478, 187-194.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.73 into pET3a vector and expressed in Escherichia coli strain BL21(DE3)pLysS Synechocystis sp. PCC 6803

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.73 additional information substrate inhibition for 4-methylumbelliferyl laminaribiose, but not for 4-methylumbelliferyl cellobiose Synechocystis sp. PCC 6803

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.73 0.058
-
 4-methylumbelliferyl cellobiose
-
 Synechocystis sp. PCC 6803
3.2.1.73 0.19
-
 4-methylumbelliferyl laminaribiose
-
 Synechocystis sp. PCC 6803

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.73 Synechocystis sp. PCC 6803
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.73 by gel filtration and ultrafiltration Synechocystis sp. PCC 6803

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.73 4-methylumbelliferyl cellobiose + H2O
-
 Synechocystis sp. PCC 6803 ?
-
 ?
3.2.1.73 4-methylumbelliferyl laminaribiose + H2O
-
 Synechocystis sp. PCC 6803 ?
-
 ?
3.2.1.73 barley beta-glucan + H2O
-
 Synechocystis sp. PCC 6803 ?
-
 ?
3.2.1.73 Glcbeta(1-3)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc + H2O
-
 Synechocystis sp. PCC 6803 glucose + Glcbeta(1-3)Glcbeta(1-4)Glcbeta(1-4)Glc
-
 ?
3.2.1.73 Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-3)Glc + H2O
-
 Synechocystis sp. PCC 6803 ?
-
 ?
3.2.1.73 lichenan + H2O
-
 Synechocystis sp. PCC 6803 ?
-
 ?
3.2.1.73 additional information does not hydrolyze laminaripentaose and cellopentaose Synechocystis sp. PCC 6803 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.73 1,3-1,4-beta-glucanase
-
 Synechocystis sp. PCC 6803

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.73 0.327
-
 4-methylumbelliferyl laminaribiose
-
 Synechocystis sp. PCC 6803
3.2.1.73 1.35
-
 4-methylumbelliferyl cellobiose
-
 Synechocystis sp. PCC 6803
3.2.1.73 3.71
-
 lichenan calculated as D-glucose units released from lichenan Synechocystis sp. PCC 6803
3.2.1.73 8.4
-
 Barley beta-glucan
-
 Synechocystis sp. PCC 6803