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Literature summary extracted from

  • Choi, K.J.; Noh, K.M.; Kim, D.E.; Ha, B.H.; Kim, E.E.; Yoon, M.Y.
    Identification of the catalytic subunit of acetohydroxyacid synthase in Haemophilus influenzae and its potent inhibitors (2007), Arch. Biochem. Biophys., 466, 24-30.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.2.1.6 His-tagged AHAS catalytic subunit , overexpression in Escherichia coli strain BL21(DE3) Haemophilus influenzae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.6 AVS-2087
-
 Haemophilus influenzae
2.2.1.6 KHG20612
-
 Haemophilus influenzae
2.2.1.6 KSW30191
-
 Haemophilus influenzae
2.2.1.6 additional information inhibitor library screening Haemophilus influenzae
2.2.1.6 sulfometuron methyl
-
 Haemophilus influenzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.6 9.2
-
 pyruvate pH 7.5, 37°C, recombinant enzyme Haemophilus influenzae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.6 Mg2+
-
 Haemophilus influenzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.2.1.6 63700
-
 x * 63700, recombinant His-tagged catalytic subunit, SDS-PAGE Haemophilus influenzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.2.1.6 2 pyruvate Haemophilus influenzae AHAS catalyzes the first common step in the biosynthetic pathway of the branched-amino acids leucine, isoleucine, and valine 2-acetolactate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.6 Haemophilus influenzae P45261 large subunit
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.6 recombinant His-tagged AHAS catalytic subunit from Escherichia coli strain BL21(DE3) to homogeneity by nickel affinity and anion exchange chromatography, and gel filtration Haemophilus influenzae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2.1.6 1.5
-
 purified recombinant enzyme Haemophilus influenzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.6 2 pyruvate AHAS catalyzes the first common step in the biosynthetic pathway of the branched-amino acids leucine, isoleucine, and valine Haemophilus influenzae 2-acetolactate + CO2
-
 ?
2.2.1.6 2 pyruvate condensation reaction Haemophilus influenzae 2-acetolactate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.2.1.6 ? x * 63700, recombinant His-tagged catalytic subunit, SDS-PAGE Haemophilus influenzae

Synonyms

EC Number Synonyms Comment Organism
2.2.1.6 acetohydroxyacid synthase
-
 Haemophilus influenzae
2.2.1.6 AHAS
-
 Haemophilus influenzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.2.1.6 37
-
 assay at Haemophilus influenzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2.1.6 7.5
-
 assay at Haemophilus influenzae

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.6 FAD dependent on Haemophilus influenzae
2.2.1.6 thiamine diphosphate dependent on Haemophilus influenzae

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.2.1.6 0.00053
-
 pH 7.5, 37°C, recombinant enzyme Haemophilus influenzae AVS-2087
2.2.1.6 0.00142
-
 pH 7.5, 37°C, recombinant enzyme Haemophilus influenzae KSW30191
2.2.1.6 0.00491
-
 pH 7.5, 37°C, recombinant enzyme Haemophilus influenzae KHG20612
2.2.1.6 0.2763
-
 pH 7.5, 37°C, recombinant enzyme Haemophilus influenzae sulfometuron methyl