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Literature summary extracted from

  • Junghanns, C.; Pecyna, M.J.; Boehm, D.; Jehmlich, N.; Martin, C.; von Bergen, M.; Schauer, F.; Hofrichter, M.; Schlosser, D.
    Biochemical and molecular genetic characterisation of a novel laccase produced by the aquatic ascomycete Phoma sp. UHH 5-1-03 (2009), Appl. Microbiol. Biotechnol., 84, 1095-1105.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 acetic acid
-
 Phoma sp.
1.10.3.2 acetone 31% (v/v) 50% inhibition Phoma sp.
1.10.3.2 acetonitrile 4.5% (v/v) 50% inhibition Phoma sp.
1.10.3.2 DMSO 40% (v/v) 50% inhibition Phoma sp.
1.10.3.2 EDTA
-
 Phoma sp.
1.10.3.2 ethanol 52% (v/v) 50% inhibition Phoma sp.
1.10.3.2 formic acid
-
 Phoma sp.
1.10.3.2 Isopropanol 60% (v/v) 50% inhibition Phoma sp.
1.10.3.2 methanol 52% (v/v) 50% inhibition Phoma sp.
1.10.3.2 Na2CO3
-
 Phoma sp.
1.10.3.2 NaCl
-
 Phoma sp.
1.10.3.2 NaF
-
 Phoma sp.
1.10.3.2 NaN3
-
 Phoma sp.
1.10.3.2 Urea
-
 Phoma sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 additional information
-
 additional information Michaelis-Menten kinetics Phoma sp.
1.10.3.2 0.008
-
 2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) pH 2.5, 65°C Phoma sp.
1.10.3.2 0.02
-
 syringaldazine pH 5.0, 65°C Phoma sp.
1.10.3.2 0.266
-
 2,6-dimethoxyphenol pH 4.0, 65°C Phoma sp.
1.10.3.2 0.284
-
 anthraquinonic dye Acid Blue 62 pH 5.5, 65°C Phoma sp.
1.10.3.2 0.648
-
 guaiacol pH 5.5, 65°C Phoma sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.10.3.2 extracellular the enzyme is secreted Phoma sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 copper a blue copper-containing oxidoreductase Phoma sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 150000 151200 about, gel filtration and mass spectrometry Phoma sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.10.3.2 additional information Phoma sp. laccases are able to oxidize a variety of substrates, ranging from mono- and polyphenols over aminophenols, methoxyphenols and aromatic amines to polycyclic aromatic hydrocarbons and certain lignin moieties ?
-
 ?
1.10.3.2 additional information Phoma sp. UHH 5-1-03 / DSM 22425 laccases are able to oxidize a variety of substrates, ranging from mono- and polyphenols over aminophenols, methoxyphenols and aromatic amines to polycyclic aromatic hydrocarbons and certain lignin moieties ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Phoma sp. B3TLA6 isolated from Saale river Germany
-
1.10.3.2 Phoma sp. UHH 5-1-03 / DSM 22425 B3TLA6 isolated from Saale river Germany
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.10.3.2 glycoprotein N-glycosylation site prediction reveals five putative sites, and nine putative sites for O-glycosylation, overview Phoma sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 native enzyme 117fold by hydrophobic interaction chromatography and gel filtration Phoma sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 7.1
-
 purified enzyme Phoma sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Phoma sp. ?
-
 ?
1.10.3.2 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Phoma sp. UHH 5-1-03 / DSM 22425 ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Phoma sp. ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Phoma sp. UHH 5-1-03 / DSM 22425 ?
-
 ?
1.10.3.2 anthraquinonic dye Acid Blue 62 + O2
-
 Phoma sp. ?
-
 ?
1.10.3.2 guaiacol + O2
-
 Phoma sp. ?
-
 ?
1.10.3.2 guaiacol + O2
-
 Phoma sp. UHH 5-1-03 / DSM 22425 ?
-
 ?
1.10.3.2 additional information laccases are able to oxidize a variety of substrates, ranging from mono- and polyphenols over aminophenols, methoxyphenols and aromatic amines to polycyclic aromatic hydrocarbons and certain lignin moieties Phoma sp. ?
-
 ?
1.10.3.2 additional information laccases are able to oxidize a variety of substrates, ranging from mono- and polyphenols over aminophenols, methoxyphenols and aromatic amines to polycyclic aromatic hydrocarbons and certain lignin moieties Phoma sp. UHH 5-1-03 / DSM 22425 ?
-
 ?
1.10.3.2 syringaldazine + O2
-
 Phoma sp. ?
-
 ?
1.10.3.2 syringaldazine + O2
-
 Phoma sp. UHH 5-1-03 / DSM 22425 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 dimer 2 * 74000-75600, about, mass spectrometry and SDS-PAGE, laccase undergoes pH-dependent dimerisation Phoma sp.
1.10.3.2 More the enzyme contains a signal sequence and a C-terminal conserved DSGL motif, tryptic peptide analysis, overview Phoma sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 65
-
-
 Phoma sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 4 45 the enzyme is most stable between 4°C and room temperature. After 288 h, the recovered activities correspond to approximately 51% at 4°C, 57% at 15°C and 49% at room temperature of the initial activity. The laccase is nearly completely inactivated at 35°C after 288 h and at 45°C after 24 h Phoma sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 1.5
-
 syringaldazine pH 5.0, 65°C Phoma sp.
1.10.3.2 4.3
-
 2,6-dimethoxyphenol pH 4.0, 65°C Phoma sp.
1.10.3.2 4.6
-
 guaiacol pH 5.5, 65°C Phoma sp.
1.10.3.2 10.6
-
 2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) pH 2.5, 65°C Phoma sp.
1.10.3.2 32.2
-
 anthraquinonic dye Acid Blue 62 pH 5.5, 65°C Phoma sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 2.5 5.5 the optimal pH values for the different substrates 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid, 2,6-dimethoxyphenol, guaiacol, syringaldazine, and anthraquinonic dye Acid Blue 62 oxidation are pH 2.5, pH 4.5, pH 5.0, pH 5.5 and pH 5.5, respectively Phoma sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.2 5 8 activity range, at pH 7.0, about 17%, 24%, 33%, 23% and 21% of the maximum oxidation rate is observed for substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid, 2,6-dimethoxyphenol, guaiacol, syringaldazine, and anthraquinonic dye Acid Blue 62, respectively, while approximately 2%, 8%, 15%, 4% and 17% of the maximum activity in oxidation of these substrates is obtained at pH 8.0 Phoma sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 4 8 the laccase is most stable at pH 4.0, pH 7.0, and 8.0, retaining about 57%, 62%, and 65% of the initial activity, respectively, after 355 h. The enzyme is less stable at pH 5.0 and pH 6.0 and is completely inactivated within 355 h at pH 2.0 Phoma sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.10.3.2 Phoma sp. isoelectric focussing
-
 8.3

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.10.3.2 0.11
-
 pH 4.0, 65°C Phoma sp. NaN3
1.10.3.2 0.23
-
 pH 4.0, 65°C Phoma sp. formic acid
1.10.3.2 3.7
-
 pH 4.0, 65°C Phoma sp. NaF
1.10.3.2 19
-
 pH 4.0, 65°C Phoma sp. EDTA
1.10.3.2 69
-
 pH 4.0, 65°C Phoma sp. Na2CO3
1.10.3.2 80
-
 pH 4.0, 65°C Phoma sp. NaCl
1.10.3.2 2250
-
 pH 4.0, 65°C Phoma sp. Urea