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Literature summary extracted from

  • Chi, Z.; Chi, Z.; Zhang, T.; Liu, G.; Yue, L.
    Inulinase-expressing microorganisms and applications of inulinases (2009), Appl. Microbiol. Biotechnol., 82, 211-220.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.7 additional information high endoinulinase productivity of 9.9 U/ml when grown in a liquid medium containing inulin as the carbon source at 30°C for 4 days Penicillium sp.
3.2.1.7 additional information maximum inulinase production (55.4 U/ml) by strain YS-1 is obtained at an agitation rate of 200 rpm and aeration of 0.75 volume/volume per minute in a stirred tank reactor (1.5 l) with a fermentation time of 60 h or inulinase activity of 50.2 U/ml is obtained from the same yeast strain under agitation (200 rpm) and aeration (0.75 volume/volume per minute) at 30°C after 60 h of fermentation in 1.5 l of fermentor when raw inulin (4.0%) from root tubers of Asparagus officinalis is used as the production medium. When strain NRRL Y-7571 is grown in the culture containing sugarcane bagasse as support and carbon source and corn steep liquor as nitrogen supplement, the extracellular inulinase concentration reaches 391.9 U/g of dry fermented bagasse. The use of soybean bran decreases the time to reach the maximum activity from 96 to 24 h and the maximum productivity achieved strain NRRL Y-7571 is 8.87 U/gds per h Kluyveromyces marxianus
3.2.1.7 additional information the fungus shows good growth on a medium containing 40% (v/v) of dandelion tap root extract composed of 50 g tap roots blended with 200 ml water and 2% yeast extract medium and produces 55 U/ml of inulinase activity in 96 h at 30°C and 150 rpm during the liquid fermentation. Dandelion tap root extract in the liquid medium induces the endoinulinase synthesis Aspergillus niger

Application

EC Number Application Comment Organism
3.2.1.7 industry yeasts can produce more inulinase than bacteria Kluyveromyces marxianus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.7 expressed on the cell surface of Saccharomyces cerevisiae EBY100 cells Pseudomonas mucidolens
3.2.1.7 INU2 gene encoding endoinulinase expressed in SUC2-deleted Saccharomyces cerevisiae Aspergillus ficuum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.7 Ag2+
-
 Penicillium sp.
3.2.1.7 Cu2+
-
 Penicillium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.7 0.2
-
 inulin
-
 Penicillium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.7 55500
-
 sequence analysis Talaromyces purpureogenus
3.2.1.7 55800
-
 sequence analysis Aspergillus ficuum
3.2.1.7 55900
-
 sequence analysis Aspergillus niger
3.2.1.7 58800
-
 sequence analysis Kluyveromyces marxianus
3.2.1.7 68000
-
-
 Penicillium sp.
3.2.1.7 75000
-
-
 Arthrobacter sp.
3.2.1.7 87900
-
 sequence analysis Arthrobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.7 Arthrobacter sp. Q9RBA5
-
-
3.2.1.7 Aspergillus ficuum O94220
-
-
3.2.1.7 Aspergillus niger A5ABL2 strain NK-126, 12, 817 and TISTR 3570
-
3.2.1.7 Aspergillus niger CBS 513.88 A5ABL2 strain NK-126, 12, 817 and TISTR 3570
-
3.2.1.7 Kluyveromyces marxianus P28999 strains ATCC 16045, ATCC 52466, NRRL Y-7571, S120, YS-1, A1 and A2
-
3.2.1.7 Penicillium sp.
-
-
-
3.2.1.7 Penicillium sp. TN-88
-
-
-
3.2.1.7 Pseudomonas mucidolens
-
-
-
3.2.1.7 Pseudomonas sp.
-
-
-
3.2.1.7 Talaromyces purpureogenus O00056
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.7 inulin + H2O
-
 Penicillium sp. ?
-
 ?
3.2.1.7 inulin + H2O
-
 Kluyveromyces marxianus ?
-
 ?
3.2.1.7 inulin + H2O
-
 Aspergillus niger ?
-
 ?
3.2.1.7 inulin + H2O
-
 Arthrobacter sp. ?
-
 ?
3.2.1.7 inulin + H2O
-
 Pseudomonas mucidolens ?
-
 ?
3.2.1.7 inulin + H2O
-
 Penicillium sp. TN-88 ?
-
 ?
3.2.1.7 inulin + H2O
-
 Aspergillus niger CBS 513.88 ?
-
 ?
3.2.1.7 inulin + H2O
-
 Aspergillus ficuum 1-kestose + ?
-
 ?
3.2.1.7 inulin + H2O
-
 Pseudomonas sp. inulooligosaccharide ranging from DP2 to DP7 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.7 endoinulinase
-
 Pseudomonas sp.
3.2.1.7 endoinulinase
-
 Penicillium sp.
3.2.1.7 endoinulinase
-
 Talaromyces purpureogenus
3.2.1.7 endoinulinase
-
 Kluyveromyces marxianus
3.2.1.7 endoinulinase
-
 Aspergillus niger
3.2.1.7 endoinulinase
-
 Arthrobacter sp.
3.2.1.7 endoinulinase
-
 Aspergillus ficuum
3.2.1.7 endoinulinase
-
 Pseudomonas mucidolens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.7 50
-
-
 Penicillium sp.
3.2.1.7 50
-
-
 Kluyveromyces marxianus
3.2.1.7 50
-
-
 Arthrobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.7 5.2
-
-
 Penicillium sp.
3.2.1.7 5.5
-
-
 Pseudomonas sp.
3.2.1.7 7.5
-
-
 Arthrobacter sp.