Literature summary extracted from

  • Canakci, S.; Kacagan, M.; Inan, K.; Belduz, A.O.; Saha, B.C.
    Cloning, purification, and characterization of a thermostable alpha-L-arabinofuranosidase from Anoxybacillus kestanbolensis AC26Sari (2008), Appl. Microbiol. Biotechnol., 81, 61-68.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.55 overexpression in Escherichia coli BL21 Anoxybacillus kestanbolensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.55 Cu2+ strong inhibition Anoxybacillus kestanbolensis
3.2.1.55 Hg2+ strong inhibition Anoxybacillus kestanbolensis
3.2.1.55 additional information activity of the enzyme is not affected by the chelating agent EDTA (10 mM) or by the reducing agents DTT and beta mercaptoethanol (10 mM) Anoxybacillus kestanbolensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.55 0.139
-
p-nitrophenyl-alpha-L-arabinofuranoside
-
Anoxybacillus kestanbolensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.55 additional information metals with a small stimulating effect on the activity are Zn2+, Mg2+, and Ca2+, no effect with Co2+, Mn2+ Anoxybacillus kestanbolensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.55 58000
-
histidine-tagged AbfAC26Sari, SDS-PAGE Anoxybacillus kestanbolensis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Anoxybacillus kestanbolensis B2D088
-
-
3.2.1.55 Anoxybacillus kestanbolensis AC26Sari B2D088
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.55 MagneHisTM Protein Purification System Anoxybacillus kestanbolensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.55 0.31
-
crude extract Anoxybacillus kestanbolensis
3.2.1.55 1112
-
HisTaq purified enzyme Anoxybacillus kestanbolensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 additional information no activity with a variety of p-nitrophenyl-glycosides, including those with beta-linkages and pyranoside conformations of arabinose Anoxybacillus kestanbolensis ?
-
?
3.2.1.55 additional information no activity with a variety of p-nitrophenyl-glycosides, including those with beta-linkages and pyranoside conformations of arabinose Anoxybacillus kestanbolensis AC26Sari ?
-
?
3.2.1.55 1,5-alpha-L-arabinotriose + 2 H2O
-
Anoxybacillus kestanbolensis 3 L-arabinose
-
?
3.2.1.55 1,5-alpha-L-arabinotriose + 2 H2O
-
Anoxybacillus kestanbolensis AC26Sari 3 L-arabinose
-
?
3.2.1.55 1,5-alpha-L-arabinotetraose + H2O
-
Anoxybacillus kestanbolensis L-arabinose
-
?
3.2.1.55 1,5-alpha-L-arabinotetraose + H2O
-
Anoxybacillus kestanbolensis AC26Sari L-arabinose
-
?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Anoxybacillus kestanbolensis p-nitrophenol + L-arabinose
-
?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Anoxybacillus kestanbolensis AC26Sari p-nitrophenol + L-arabinose
-
?
3.2.1.55 oat spelt xylan + H2O
-
Anoxybacillus kestanbolensis L-arabinose + ?
-
?
3.2.1.55 oat spelt xylan + H2O
-
Anoxybacillus kestanbolensis AC26Sari L-arabinose + ?
-
?
3.2.1.55 arabinan + H2O
-
Anoxybacillus kestanbolensis L-arabinose + ?
-
?
3.2.1.55 arabinoxylan + H2O
-
Anoxybacillus kestanbolensis L-arabinose + ?
-
?
3.2.1.55 1,5-alpha-L-arabinobiose + H2O
-
Anoxybacillus kestanbolensis L-arabinose + ?
-
?
3.2.1.55 1,5-alpha-L-arabinopentaose + H2O
-
Anoxybacillus kestanbolensis L-arabinose
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.55 alpha-L-arabinofuranosidase
-
Anoxybacillus kestanbolensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.55 65
-
-
Anoxybacillus kestanbolensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.55 45 85
-
Anoxybacillus kestanbolensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.55 45 85 the enzyme maintains full activity at 50°C, 96% activity at 55°C, 60°C, and 65°C, and 80% activity at 70°C for 24 h Anoxybacillus kestanbolensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.55 5.5
-
-
Anoxybacillus kestanbolensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.55 4.5 9
-
Anoxybacillus kestanbolensis