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Literature summary extracted from

  • Cheng, Y.M.; Hong, T.Y.; Liu, C.C.; Meng, M.
    Cloning and functional characterization of a complex endo-beta-1,3-glucanase from Paenibacillus sp. (2009), Appl. Microbiol. Biotechnol., 81, 1051-1061.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.6 drug development the various truncated proteins of LamA also show inhibition on the mycelium growth of Rhizopus solani, a pathogen infecting a broad range of many crops, suggesting the application potential of LamA as an antifungal agent in many aspects Paenibacillus sp. CCRC 17245

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.6 expression of five truncated proteins of LamA in Escherichia coli BL21(DE3) Paenibacillus sp. CCRC 17245

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.6 additional information
-
 Laminarin 1.1 mg/ml, truncated protein C of LamA Paenibacillus sp. CCRC 17245
3.2.1.6 additional information
-
 Laminarin 1.8 mg/ml, truncated protein CF of LamA Paenibacillus sp. CCRC 17245
3.2.1.6 additional information
-
 Laminarin 2.4 mg/ml, truncated protein CB3 of LamA Paenibacillus sp. CCRC 17245
3.2.1.6 additional information
-
 Laminarin 2.4 mg/ml, truncated protein SC of LamA Paenibacillus sp. CCRC 17245
3.2.1.6 additional information
-
 Laminarin 2.9 mg/ml, truncated protein CBF of LamA Paenibacillus sp. CCRC 17245

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.6 38200
-
 calculated molecular mass of expressed C of LamA Paenibacillus sp. CCRC 17245
3.2.1.6 51600
-
 calculated molecular mass of expressed CF of LamA Paenibacillus sp. CCRC 17245
3.2.1.6 77000
-
 calculated molecular mass of expressed SC of LamA Paenibacillus sp. CCRC 17245
3.2.1.6 92000
-
 calculated molecular mass of expressed CB3 of LamA Paenibacillus sp. CCRC 17245
3.2.1.6 144600
-
 calculated molecular mass of expressed CBF of LamA Paenibacillus sp. CCRC 17245

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.39 additional information Paenibacillus sp. CCRC 17245 the catalytic module has an inhibitory effect on the growth of Candida albicans and Rhizoctonia solani. The presence of the carbohydrate-binding module and the analogue of coagulation factor Fa5/8C enhance the inhibitory effect ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.6 Paenibacillus sp. CCRC 17245 Q000P7
-
-
3.2.1.39 Paenibacillus sp. CCRC 17245 Q000P7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.6 immobilized Ni2+ affinity chromatography, gel filtration chromatography Paenibacillus sp. CCRC 17245

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.6 additional information
-
 0.3 U/nmol with substrate curdlan, truncated protein C of LamA Paenibacillus sp. CCRC 17245
3.2.1.6 additional information
-
 0.3 U/nmol with substrate pachyman, truncated protein C of LamA Paenibacillus sp. CCRC 17245
3.2.1.6 additional information
-
 0.4 U/nmol with substrate barley beta-glucan, truncated protein C of LamA Paenibacillus sp. CCRC 17245
3.2.1.6 additional information
-
 0.4 U/nmol with substrate lichenan, truncated protein C of LamA Paenibacillus sp. CCRC 17245
3.2.1.6 additional information
-
 0.4 U/nmol with substrate lichenan, truncated protein CF of LamA Paenibacillus sp. CCRC 17245
3.2.1.6 additional information
-
 0.4 U/nmol with substrate pachyman, truncated protein CF of LamA Paenibacillus sp. CCRC 17245
3.2.1.6 additional information
-
 0.4 U/nmol with substrate zymosan A, truncated protein C of LamA Paenibacillus sp. CCRC 17245
3.2.1.6 additional information
-
 0.5 U/nmol with substrate barley beta-glucan, truncated protein CF of LamA Paenibacillus sp. CCRC 17245
3.2.1.6 additional information
-
 0.5 U/nmol with substrate zymosan A, truncated protein CF of LamA Paenibacillus sp. CCRC 17245
3.2.1.6 additional information
-
 0.6 U/nmol with substrate curdlan, truncated protein CF of LamA Paenibacillus sp. CCRC 17245
3.2.1.6 additional information
-
 0.6 U/nmol with substrate pachyman, truncated protein SC of LamA Paenibacillus sp. CCRC 17245
3.2.1.6 additional information
-
 0.7 U/nmol with substrate barley beta-glucan, truncated protein SC of LamA Paenibacillus sp. CCRC 17245
3.2.1.6 additional information
-
 0.7 U/nmol with substrate curdlan, truncated protein SC of LamA Paenibacillus sp. CCRC 17245
3.2.1.6 additional information
-
 0.7 U/nmol with substrate lichenan, truncated protein SC of LamA Paenibacillus sp. CCRC 17245
3.2.1.6 additional information
-
 0.7 U/nmol with substrate zymosan A, truncated protein SC of LamA Paenibacillus sp. CCRC 17245
3.2.1.6 additional information
-
 0.8 U/nmol with substrate pachyman, truncated protein CB3 of LamA Paenibacillus sp. CCRC 17245
3.2.1.6 additional information
-
 0.8 U/nmol with substrate zymosan A, truncated protein CB3 of LamA Paenibacillus sp. CCRC 17245
3.2.1.6 additional information
-
 0.9 U/nmol with substrate barley beta-glucan, truncated protein CB3 of LamA Paenibacillus sp. CCRC 17245
3.2.1.6 additional information
-
 0.9 U/nmol with substrate lichenan, truncated protein CB3 of LamA Paenibacillus sp. CCRC 17245
3.2.1.6 additional information
-
 1.0 U/nmol with substrate curdlan, truncated protein CB3 of LamA Paenibacillus sp. CCRC 17245
3.2.1.6 additional information
-
 1.3 U/nmol with substrate lichenan, truncated protein CBF of LamA Paenibacillus sp. CCRC 17245
3.2.1.6 additional information
-
 1.3 U/nmol with substrate pachyman, truncated protein CBF of LamA Paenibacillus sp. CCRC 17245
3.2.1.6 additional information
-
 1.4 U/nmol with substrate laminarin, truncated protein C of LamA Paenibacillus sp. CCRC 17245
3.2.1.6 additional information
-
 1.5 U/nmol with substrate barley beta-glucan, truncated protein CBF of LamA Paenibacillus sp. CCRC 17245
3.2.1.6 additional information
-
 1.6 U/nmol with substrate zymosan A, truncated protein CBF of LamA Paenibacillus sp. CCRC 17245
3.2.1.6 additional information
-
 1.7 U/nmol with substrate laminarin, truncated protein CF of LamA Paenibacillus sp. CCRC 17245
3.2.1.6 additional information
-
 2.0 U/nmol with substrate curdlan, truncated protein CBF of LamA Paenibacillus sp. CCRC 17245
3.2.1.6 additional information
-
 2.6 U/nmol with substrate laminarin, truncated protein CB3 of LamA Paenibacillus sp. CCRC 17245
3.2.1.6 additional information
-
 3.7 U/nmol with substrate laminarin, truncated protein CBF of LamA Paenibacillus sp. CCRC 17245
3.2.1.6 additional information
-
 4.6 U/nmol with substrate laminarin, truncated protein SC of LamA Paenibacillus sp. CCRC 17245
3.2.1.6 additional information
-
 no activity with carboxymethylcellulose and xylan of all truncated proteins of LamA Paenibacillus sp. CCRC 17245

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.6 barley beta-glucan + H2O
-
 Paenibacillus sp. CCRC 17245 ?
-
 ?
3.2.1.6 curdlan + H2O from Alcaligenes faecalis Paenibacillus sp. CCRC 17245 ?
-
 ?
3.2.1.6 laminarin + H2O from Laminaria digitata Paenibacillus sp. CCRC 17245 laminaritriose
-
 ?
3.2.1.6 lichenan + H2O
-
 Paenibacillus sp. CCRC 17245 ?
-
 ?
3.2.1.6 additional information no activity with carboxymethylcellulose and xylan of all truncated proteins of LamA Paenibacillus sp. CCRC 17245 ?
-
 ?
3.2.1.6 pachyman + H2O
-
 Paenibacillus sp. CCRC 17245 ?
-
 ?
3.2.1.6 zymosan A + H2O from Saccharomyces cerevisiae Paenibacillus sp. CCRC 17245 ?
-
 ?
3.2.1.39 avicel + H2O
-
 Paenibacillus sp. CCRC 17245 ?
-
 ?
3.2.1.39 barley beta-glucan + H2O
-
 Paenibacillus sp. CCRC 17245 ?
-
 ?
3.2.1.39 curdlan + H2O
-
 Paenibacillus sp. CCRC 17245 ?
-
 ?
3.2.1.39 laminarin + H2O preferred substrate Paenibacillus sp. CCRC 17245 laminaritriose main product ?
3.2.1.39 lichenan + H2O
-
 Paenibacillus sp. CCRC 17245 ?
-
 ?
3.2.1.39 additional information the catalytic module has an inhibitory effect on the growth of Candida albicans and Rhizoctonia solani. The presence of the carbohydrate-binding module and the analogue of coagulation factor Fa5/8C enhance the inhibitory effect Paenibacillus sp. CCRC 17245 ?
-
 ?
3.2.1.39 additional information the catalytic module specifically hydrolyzes beta-1,3- and beta-1,3-1,4-glucan. The catalytic module has an inhibitory effect on the growth of Candida albicans and Rhizoctonia solani. The presence of the carbohydrate-binding module and the analogue of coagulation factor Fa5/8C enhance the inhibitory effect Paenibacillus sp. CCRC 17245 ?
-
 ?
3.2.1.39 pachyman + H2O
-
 Paenibacillus sp. CCRC 17245 ?
-
 ?
3.2.1.39 zymosan A + H2O
-
 Paenibacillus sp. CCRC 17245 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.39 More putative functional units of the single protein strain include, from N to C terminus, a leader peptide, three repeats of the S-layer homologous module, a catalytic module of glycoside hydrolase family 16, four repeats of the carbohydrate-binding module of family CBM_4_9, and an analogue of coagulation factor Fa5/8C Paenibacillus sp. CCRC 17245

Synonyms

EC Number Synonyms Comment Organism
3.2.1.6 endo-1,3-beta-glucanase
-
 Paenibacillus sp. CCRC 17245
3.2.1.6 LamA
-
 Paenibacillus sp. CCRC 17245
3.2.1.6 laminarinase
-
 Paenibacillus sp. CCRC 17245
3.2.1.39 LamA
-
 Paenibacillus sp. CCRC 17245

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.6 40
-
 for truncated protein CBF, pH 5.5 Paenibacillus sp. CCRC 17245
3.2.1.6 50
-
 for truncated protein C, pH 5.5 Paenibacillus sp. CCRC 17245
3.2.1.39 40
-
 catalytic module Paenibacillus sp. CCRC 17245

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.6 5.5
-
 toward laminarin hydrolysis for truncated proteins CBF and C Paenibacillus sp. CCRC 17245
3.2.1.39 5.5 6 catalytic module Paenibacillus sp. CCRC 17245