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Literature summary extracted from
- Lysostaphin: an antistaphylococcal agent (2008), Appl. Microbiol. Biotechnol., 80, 555-561.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.4.24.75 | analysis | improvement of lipid extraction of staphylococcal cells | Staphylococcus simulans |
| 3.4.24.75 | diagnostics | detection of Staphylococcus aureus | Staphylococcus simulans |
| 3.4.24.75 | food industry | antistaphylococcal agent | Staphylococcus simulans |
| 3.4.24.75 | medicine | potential therapeutic agent against antibiotic-resistant Staphylococcus aureus infections | Staphylococcus simulans |
| 3.4.24.75 | additional information | antistaphylococcal agent in clinical laboratories | Staphylococcus simulans |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.24.75 | expression in Bacillus sphaericus | Staphylococcus simulans |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.75 | Zn2+ | - |
Staphylococcus epidermidis |  |
| 3.4.24.75 | Zn2+ | - |
Staphylococcus simulans | |
| 3.5.1.28 | Zn2+ | - |
Staphylococcus simulans | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.75 | 27000 | - |
- |
Staphylococcus simulans |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.24.75 | Peptidoglycan + H2O | Staphylococcus simulans | activities of three enzymes namely, glycylglycine endopeptidase, endo-beta-N-acetyl glucosamidase and N-acteyl muramyl-L-alanine amidase. Lysostaphin rapidly lyses actively growing and non-dividing cells including staphylococci in biofilms. Glycylglycine endopeptidase lyses staphylococcal cells by hydrolyzing glycylglycine bonds in the polyglycine bridges which form cross links between glycopeptide chains in the cell wall peptidoglycan of Staphylococcus aureus cells. Lytic principle of lysostaphin is a peptidase which liberates N-terminal glycine and alanine from Staphylococcus aureus cell wall. Hexosaminidase is present in lysostaphin preparation is specific for the gluocainyl-muramic acid bond of the bacterial carbohydrate backbone | Fragments of peptidoglycan | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.24.75 | Staphylococcus epidermidis | - |
- |
- |
| 3.4.24.75 | Staphylococcus simulans | - |
- |
- |
| 3.5.1.28 | Staphylococcus simulans | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.24.75 | proteolytic modification | lysostaphin is secreted as a proenzyme. Proteolytic cleavage of 13 N-terminal tandem repeats generates mature lysostaphin with 2 functionally separable domains | Staphylococcus simulans |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.24.75 | lysostaphin can be purified from Staphylococcus simulans strains | Staphylococcus simulans |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.75 | additional information | - |
activities of three enzymes namely, glycylglycine endopeptidase, endo-beta-N-acetyl glucosamidase and N-acteyl muramyl-L-alanine amidase. Lysostaphin rapidly lyses actively growing and non-dividing cells including staphylococci in biofilms. Glycylglycine endopeptidase lyses staphylococcal cells by hydrolyzing glycylglycine bonds in the polyglycine bridges which form cross links between glycopeptide chains in the cell wall peptidoglycan of Staphylococcus aureus cells. Lytic principle of lysostaphin is a peptidase which liberates N-terminal glycine and alanine from Staphylococcus aureus cell wall. Hexosaminidase is present in lysostaphin preparation is specific for the gluocainyl-muramic acid bond of the bacterial carbohydrate backbone | Staphylococcus simulans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.24.75 | Peptidoglycan + H2O | activities of three enzymes namely, glycylglycine endopeptidase, endo-beta-N-acetyl glucosamidase and N-acteyl muramyl-L-alanine amidase. Lysostaphin rapidly lyses actively growing and non-dividing cells including staphylococci in biofilms. Glycylglycine endopeptidase lyses staphylococcal cells by hydrolyzing glycylglycine bonds in the polyglycine bridges which form cross links between glycopeptide chains in the cell wall peptidoglycan of Staphylococcus aureus cells. Lytic principle of lysostaphin is a peptidase which liberates N-terminal glycine and alanine from Staphylococcus aureus cell wall. Hexosaminidase is present in lysostaphin preparation is specific for the gluocainyl-muramic acid bond of the bacterial carbohydrate backbone | Staphylococcus simulans | Fragments of peptidoglycan | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.24.75 | lysostaphin | - |
Staphylococcus simulans |
| 3.4.24.75 | r-lysostaphin | recombinant lysostaphin | Staphylococcus simulans |
| 3.5.1.28 | lysostaphin | activities: N-acetyl muramyl-L-alanine amidase, glycylglycine endopeptidase and endo-beta-N-acetyl glucosamidase | Staphylococcus simulans |
| 3.5.1.28 | N-acetyl muramyl-L-alanine amidase | - |
Staphylococcus simulans |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.75 | 5 | - |
half-life of the staphylolytic activity approximately 2 months | Staphylococcus epidermidis |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.75 | 37 | - |
stable for 72 h, recombinant enzyme | Staphylococcus simulans |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.28 | 5 | - |
half-life of staphylolytic activity: approximately 2 months (pH 4) | Staphylococcus simulans |
| 3.5.1.28 | 37 | - |
recombinant lysostaphin: stable for 72 h | Staphylococcus simulans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.75 | 4 | - |
half-life of the staphylolytic activity approximately 2 months | Staphylococcus epidermidis |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.28 | 4 | - |
half-life of staphylolytic activity: approximately 2 months (5°C) | Staphylococcus simulans |
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