EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.11.1.7 | syringaldehyde | the decolorization activity of AnaPX toward azo dyes is significantly enhanced 2-50fold in the presence of syringaldehyde | Anabaena sp. | |
1.11.1.19 | syringaldehyde | the presence of syringaldehyde drastically enhances the rate of decolorization of Reactive Black 5, Reactive Orange 14, Reactive Red 120, Reactive Green 19, and Acid Red with 50fold, 9fold, 15fold, 2fold, and 7fold improvements, respectively | Anabaena sp. |
EC Number | Application | Comment | Organism |
---|---|---|---|
1.11.1.19 | additional information | AnaPX is a useful alternative of horseradish peroxidase or fungal DyPs | Anabaena sp. |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.11.1.7 | expressed in Escherichia coli BL21(DE3) cells | Anabaena sp. |
1.11.1.19 | expressed in Escherichia coli BL21(DE3) cells | Anabaena sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.11.1.7 | diethyl dicarbonate | less than 50% inhibition at 50 mM | Anabaena sp. | |
1.11.1.7 | Fe2+ | 98.8% inhibition at 5 mM | Anabaena sp. | |
1.11.1.7 | KCN | very low sensitivity, causes only 9% and 14% inhibition of the activity at 1 and 10 mM, respectively | Anabaena sp. | |
1.11.1.7 | Mn2+ | 57.1% inhibition at 5 mM | Anabaena sp. | |
1.11.1.7 | additional information | the enzyme is not sensitive to the prototypical catalase inhibitor 3-amino-1, 2, 4,-triazole in presence of ascorbic acid (1.0 mM) | Anabaena sp. | |
1.11.1.7 | NaN3 | 82% inhibition 10 mM | Anabaena sp. | |
1.11.1.7 | phenylhydrazine | complete inhibition at 1 mM | Anabaena sp. | |
1.11.1.19 | diethyl dicarbonate | less than 50% inhibition at 50 mM | Anabaena sp. | |
1.11.1.19 | Fe2+ | AnaPX is highly sensitive to Fe2+ (98.8% inhibition at 5 mM) | Anabaena sp. | |
1.11.1.19 | KCN | AnaPX shows very low sensitivity to KCN which causes only 9% and 14% inhibition of the activity at 1 and 10 mM, respectively | Anabaena sp. | |
1.11.1.19 | Mn2+ | 57.1% inhibition at 5 mM | Anabaena sp. | |
1.11.1.19 | additional information | the enzyme is not sensitive to the prototypical catalase inhibitor 3-amino-1,2,4,-triazole in the presence of ascorbic acid (1.0 mM), metal chelating and sulfhydryl reagents do not significantly affect activity | Anabaena sp. | |
1.11.1.19 | NaN3 | AnaPX is highly sensitive to NaN3 (82% inhibition at 10 mM) | Anabaena sp. | |
1.11.1.19 | phenylhydrazine | AnaPX is highly sensitive to the suicide substrate phenylhydrazine (complete inhibition at 1 mM) | Anabaena sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.11.1.7 | 0.0036 | - |
Reactive Blue 5 | at pH 4.8 | Anabaena sp. | |
1.11.1.7 | 0.0058 | - |
H2O2 | at pH 4.8 | Anabaena sp. | |
1.11.1.19 | 0.0036 | - |
Reactive Blue 5 | in 50 mM citrate buffer (pH 4.0-4.4), at 37°C | Anabaena sp. | |
1.11.1.19 | 0.0058 | - |
H2O2 | in 50 mM citrate buffer (pH 4.0-4.4), at 37°C | Anabaena sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.11.1.7 | 53280 | - |
4 * 53280, MALDI-TOF mass spectrometry | Anabaena sp. |
1.11.1.7 | 53368 | - |
4 * 53368, calculated from amino acid sequence | Anabaena sp. |
1.11.1.7 | 54000 | - |
4 * 54000, SDS-PAGE | Anabaena sp. |
1.11.1.19 | 53280 | - |
4 * 53280, native enzyme, MALDI-TOF spectrometry | Anabaena sp. |
1.11.1.19 | 53368 | - |
4 * 53368, deduced from amino acid sequence | Anabaena sp. |
1.11.1.19 | 54000 | - |
4 * 54000, native enzyme, SDS-PAGE | Anabaena sp. |
1.11.1.19 | 209000 | - |
native enzyme, gel filtration | Anabaena sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.11.1.7 | Anabaena sp. | - |
strain PCC 7120 | - |
1.11.1.19 | Anabaena sp. | Q8YWM0 | strain PCC7120 (ATCC 27893) | - |
EC Number | Oxidation Stability | Organism |
---|---|---|
1.11.1.7 | retains more than 50% of its activity at 2.5 mM H2O2 | Anabaena sp. |
1.11.1.19 | the enzyme retains more than 50% of its activity at 2.5 mM H2O2 | Anabaena sp. |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.11.1.7 | DEAE-Toyopearl column chromatography, ammonium sulfate precipitation, butyl-Toyopearl column chromatography, and hydroxylapatite column chromatography | Anabaena sp. |
1.11.1.19 | Toyopearl DEAE-650M column chromatography, Toyopearl butyl-650M column chromatography, and hydroxylapatite column chromatography | Anabaena sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.11.1.7 | 5.6 | - |
recombinant enzyme from crude extract | Anabaena sp. |
1.11.1.7 | 283 | - |
recombinant enzyme after 50.5fold purification | Anabaena sp. |
1.11.1.19 | 5.6 | - |
recombinant enzyme from crude extract, using Reactive Blue 5 as substrate, pH 4.0-4.4, at 37°C | Anabaena sp. |
1.11.1.19 | 54 | - |
recombinant enzyme, in 50 mM citrate buffer (pH 4.0-4.4), at 37°C, using syringaldehyde as substrate | Anabaena sp. |
1.11.1.19 | 71 | - |
recombinant enzyme, in 50 mM citrate buffer (pH 4.0-4.4), at 37°C, using 2,6-dimethoxyphenol as substrate | Anabaena sp. |
1.11.1.19 | 204 | - |
recombinant enzyme, in 50 mM citrate buffer (pH 4.0-4.4), at 37°C, using 2,2'-azino-bis(3-ethylbenzthiazoline)-6-sulfonic acid as substrate | Anabaena sp. |
1.11.1.19 | 216 | - |
recombinant enzyme, in 50 mM citrate buffer (pH 4.0-4.4), at 37°C, using pyrogallol as substrate | Anabaena sp. |
1.11.1.19 | 230 | - |
recombinant enzyme, in 50 mM citrate buffer (pH 4.0-4.4), at 37°C, using guaiacol as substrate | Anabaena sp. |
1.11.1.19 | 283 | - |
recombinant enzyme after 50.5fold purification, using Reactive Blue 5 as substrate, pH 4.0-4.4, at 37°C | Anabaena sp. |
1.11.1.19 | 290 | - |
recombinant enzyme, in 50 mM citrate buffer (pH 4.0-4.4), at 37°C, using L-ascorbate as substrate | Anabaena sp. |
1.11.1.19 | 308 | - |
recombinant enzyme, in 50 mM citrate buffer (pH 4.0-4.4), at 37°C, using D-isoascorbate as substrate | Anabaena sp. |
1.11.1.19 | 308 | - |
recombinant enzyme, in 50 mM citrate buffer (pH 4.0-4.4), at 37°C, using NADPH as substrate | Anabaena sp. |
1.11.1.19 | 374 | - |
recombinant enzyme, in 50 mM citrate buffer (pH 4.0-4.4), at 37°C, using NADH as substrate | Anabaena sp. |
1.11.1.19 | 1478 | - |
recombinant enzyme, in 50 mM citrate buffer (pH 4.0-4.4), at 37°C, using 4-aminoantipyrine as substrate | Anabaena sp. |
EC Number | Storage Stability | Organism |
---|---|---|
1.11.1.7 | 4°C, in 50 mM potassium phosphate (pH 7.0), 40 days, less than 10% loss of activity | Anabaena sp. |
1.11.1.19 | 4°C, 50 mM potassium phosphate buffer (pH 7.0), 40 days, 10% loss of activity | Anabaena sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.11.1.7 | 2,6-dimethoxyphenol + H2O2 | - |
Anabaena sp. | ? | - |
? | |
1.11.1.7 | 4-aminoantipyrine + H2O2 | 643% activity compared to guaiacol | Anabaena sp. | ? | - |
? | |
1.11.1.7 | ABTS + H2O2 | 89% activity compared to guaiacol | Anabaena sp. | ? | - |
? | |
1.11.1.7 | Acid Blue 45 + H2O2 | - |
Anabaena sp. | ? | - |
? | |
1.11.1.7 | D-iso-ascorbate + H2O2 | 134% activity compared to guaiacol | Anabaena sp. | ? | - |
? | |
1.11.1.7 | guaiacol + H2O2 | 100% activity | Anabaena sp. | ? | - |
? | |
1.11.1.7 | L-ascorbate + H2O2 | 126% activity compared to guaiacol | Anabaena sp. | ? | - |
? | |
1.11.1.7 | additional information | no activity with Mn2+ and veratryl alcohol as electron donors | Anabaena sp. | ? | - |
? | |
1.11.1.7 | NADH + H2O2 | 163% activity compared to guaiacol | Anabaena sp. | ? | - |
? | |
1.11.1.7 | NADPH + H2O2 | 134% activity compared to guaiacol | Anabaena sp. | ? | - |
? | |
1.11.1.7 | pyrogallol + H2O2 | 93% activity compared to guaiacol | Anabaena sp. | purpurogallin + H2O | - |
? | |
1.11.1.7 | Reactive Blue 114 + H2O2 | - |
Anabaena sp. | ? | - |
? | |
1.11.1.7 | Reactive Blue 119 + H2O2 | - |
Anabaena sp. | ? | - |
? | |
1.11.1.7 | Reactive Blue 4 + H2O2 | - |
Anabaena sp. | ? | - |
? | |
1.11.1.7 | Reactive Blue 5 + H2O2 | 123% activity compared to guaiacol, the apparent optimum concentration of H2O2 required for the decolorization of Reactive Blue 5 by AnaPX is 0.4 mM | Anabaena sp. | ? | - |
? | |
1.11.1.7 | syringaldehyde + H2O2 | 23% activity compared to guaiacol | Anabaena sp. | ? | - |
? | |
1.11.1.19 | 2,2'-azino-bis(3-ethylbenzthiazoline)-6-sulfonic acid + H2O2 + H+ | 89% activity compared to guaiacol | Anabaena sp. | ? | - |
? | |
1.11.1.19 | 2,6-dimethoxyphenol + H2O2 + H+ | 31% activity compared to guaiacol | Anabaena sp. | ? | - |
? | |
1.11.1.19 | 4-aminoantipyrine + H2O2 + H+ | 643% activity compared to guaiacol | Anabaena sp. | ? | - |
? | |
1.11.1.19 | Acid Blue 45 + H2O2 + H+ | 98% decolorization rate compared to Reactive Blue 5 | Anabaena sp. | oxidized Acid Blue 45 + H2O | - |
? | |
1.11.1.19 | Acid Red 151 + H2O2 + H+ | 12% decolorization rate compared to Reactive Blue 5 | Anabaena sp. | oxidized Acid Red 151 + H2O | - |
? | |
1.11.1.19 | Congo Red + H2O2 + H+ | 12% decolorization rate compared to Reactive Blue 5 | Anabaena sp. | oxidized Congo Red + H2O | - |
? | |
1.11.1.19 | D-isoascorbate + H2O2 + H+ | 134% activity compared to guaiacol | Anabaena sp. | ? | - |
? | |
1.11.1.19 | Direct Sky Blue 6B + H2O2 + H+ | 50% decolorization rate compared to Reactive Blue 5 | Anabaena sp. | oxidized Direct Sky Blue 6B + H2O | - |
? | |
1.11.1.19 | guaiacol + H2O2 + H+ | 100% activity | Anabaena sp. | ? | - |
? | |
1.11.1.19 | L-ascorbate + H2O2 + H+ | 126% activity compared to guaiacol | Anabaena sp. | ? | - |
? | |
1.11.1.19 | additional information | the decolorization activity of AnaPX toward azo dyes is relatively low. No decolorization of Toluidine Blue O, Reactive Red 33, and Reactive Yellow 2 is observed | Anabaena sp. | ? | - |
? | |
1.11.1.19 | Procion Blue H-ERD + H2O2 + H+ | 90% decolorization rate compared to Reactive Blue 5 | Anabaena sp. | oxidized Procion Blue H-ERD + H2O | - |
? | |
1.11.1.19 | Procion Blue H-EXL + H2O2 + H+ | 72% decolorization rate compared to Reactive Blue 5 | Anabaena sp. | oxidized Procion Blue H-EXL + H2O | - |
? | |
1.11.1.19 | pyrogallol + H2O2 + H+ | 93% activity compared to guaiacol | Anabaena sp. | ? | - |
? | |
1.11.1.19 | Reactive Black 5 + H2O2 + H+ | 8% decolorization rate compared to Reactive Blue 5 | Anabaena sp. | oxidized Reactive Black 5 + H2O | - |
? | |
1.11.1.19 | Reactive Blue 114 + H2O2 + H+ | 187% decolorization rate compared to Reactive Blue 5 | Anabaena sp. | oxidized Reactive Blue 114 + H2O | - |
? | |
1.11.1.19 | Reactive Blue 19 + H2O2 + H+ | 153% decolorization rate compared to Reactive Blue 5 | Anabaena sp. | oxidized Reactive Blue 19 + H2O | - |
? | |
1.11.1.19 | Reactive Blue 4 + H2O2 + H+ | 64% decolorization rate compared to Reactive Blue 5 | Anabaena sp. | oxidized Reactive Blue 4 + H2O | - |
? | |
1.11.1.19 | Reactive Blue 5 + H2O2 + H+ | 123% activity compared to guaiacol, the apparent optimum concentration of H2O2 required for the decolorization of Reactive Blue 5 by AnaPX is 0.4 mM | Anabaena sp. | oxidized Reactive Blue 5 + H2O | - |
? | |
1.11.1.19 | Reactive Green 19 + H2O2 + H+ | 35% decolorization rate compared to Reactive Blue 5 | Anabaena sp. | oxidized Reactive Green 19 + H2O | - |
? | |
1.11.1.19 | Reactive Orange 14 + H2O2 + H+ | 10% decolorization rate compared to Reactive Blue 5 | Anabaena sp. | oxidized Reactive Orange 14 + H2O | - |
? | |
1.11.1.19 | Reactive Red 120 + H2O2 + H+ | 5% decolorization rate compared to Reactive Blue 5 | Anabaena sp. | oxidized Reactive Red 120 + H2O | - |
? | |
1.11.1.19 | Reactive Yellow 86 + H2O2 + H+ | 3% decolorization rate compared to Reactive Blue 5 | Anabaena sp. | oxidized Reactive Yellow 86 + H2O | - |
? | |
1.11.1.19 | syringaldehyde + H2O2 + H+ | 23% activity compared to guaiacol | Anabaena sp. | oxidized syringaldehyde + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.11.1.7 | homotetramer | 4 * 54000, SDS-PAGE | Anabaena sp. |
1.11.1.7 | homotetramer | 4 * 53280, MALDI-TOF mass spectrometry | Anabaena sp. |
1.11.1.7 | homotetramer | 4 * 53368, calculated from amino acid sequence | Anabaena sp. |
1.11.1.19 | homotetramer | 4 * 53280, native enzyme, MALDI-TOF spectrometry | Anabaena sp. |
1.11.1.19 | homotetramer | 4 * 53368, deduced from amino acid sequence | Anabaena sp. |
1.11.1.19 | homotetramer | 4 * 54000, native enzyme, SDS-PAGE | Anabaena sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.11.1.7 | AnaPX | - |
Anabaena sp. |
1.11.1.19 | AnaPX | - |
Anabaena sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.11.1.7 | 35 | - |
- |
Anabaena sp. |
1.11.1.19 | 35 | - |
- |
Anabaena sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.11.1.7 | 30 | 60 | the enzyme is considerably more stable at 30°C and 40°C where it retains more than 90% of its activity, the enzyme loses more than 90% of its activity after incubation at 50°C and 60°C for 3 h | Anabaena sp. |
1.11.1.19 | 30 | 60 | the enzyme is considerably stable at 30°C and 40°C where it retains more than 90% of its activity, while the enzyme loses more than 90% of its activity after incubation at 50°C and 60°C for 3 h | Anabaena sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.11.1.19 | 384 | - |
H2O2 | in 50 mM citrate buffer (pH 4.0-4.4), at 37°C | Anabaena sp. | |
1.11.1.19 | 384 | - |
Reactive Blue 5 | in 50 mM citrate buffer (pH 4.0-4.4), at 37°C | Anabaena sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.11.1.7 | 4 | 5 | - |
Anabaena sp. |
1.11.1.19 | 4 | 4.4 | - |
Anabaena sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.11.1.7 | 3.5 | 9.5 | when maintained at 40°C for 20 min, the enzyme is stable at pH values between 3.5 and 9.5 | Anabaena sp. |
1.11.1.19 | 3.5 | 9.5 | when maintained at 40°C for 20 min, the enzyme is stable at pH values between 3.5 and 9.5. The enzyme shows 20% relative activity at pH 3.0 and 10.0, 40% relative activity at pH 4.0, about 65% relative activity at pH 4.5 and 9.0, about 80% relative activity at pH 5.0, more than 90% relative activity at pH 6.0-8.0 | Anabaena sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.11.1.7 | heme | iron protoporphyrin-containing heme peroxidase | Anabaena sp. | |
1.11.1.19 | heme | iron protoporphyrin-containing heme peroxidase, the heme content is estimated to be 0.91 mol per mole of protein | Anabaena sp. |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.11.1.7 | Anabaena sp. | isoelectric focusing | - |
3.7 |
1.11.1.19 | Anabaena sp. | isoelectric focusing | - |
3.7 |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.11.1.19 | 66000 | - |
H2O2 | in 50 mM citrate buffer (pH 4.0-4.4), at 37°C | Anabaena sp. | |
1.11.1.19 | 120000 | - |
Reactive Blue 5 | in 50 mM citrate buffer (pH 4.0-4.4), at 37°C | Anabaena sp. |