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Literature summary extracted from

  • Kim, S.W.; Fushinobu, S.; Zhou, S.; Wakagi, T.; Shoun, H.
    Eukaryotic nirK genes encoding copper-containing nitrite reductase: originating from the protomitochondrion? (2009), Appl. Environ. Microbiol., 75, 2652-2658.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.2.1 CO 20% residual activity Fusarium oxysporum
1.7.2.1 diethyldithiocarbamate 1 mM, 10% residual activity Fusarium oxysporum
1.7.2.1 EDTA 1 mM, 60% resiudal activity Fusarium oxysporum
1.7.2.1 KCN 1 mM, 5% residual activity Fusarium oxysporum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.7.2.1 mitochondrion the first 65 amino acid residues of theN-terminal domain constitute a mitochondrial targeting signal, sequence analysis Fusarium oxysporum 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.2.1 copper ligands of the type 1 and 2 Cu centers are completely conserved in NiR. They are His253, Cys294, His302, and Met307 for the type 1 Cu center and His258, His293, and His456 for type 2.Asp256 and His405 residues are important for enzyme catalysis by forming a hydrogen bond network on the type 2 Cu center. The EPR spectrum is also characteristic of NirK proteins that contain both type 1 and type 2 copper centers Fusarium oxysporum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.2.1 53705
-
 x * 53705, calculated, x * 60000, SDS-PAGE Fusarium oxysporum
1.7.2.1 60000
-
 x * 53705, calculated, x * 60000, SDS-PAGE Fusarium oxysporum
1.7.2.1 900000
-
 gel filtration, aggregate Fusarium oxysporum

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.1 Fusarium oxysporum A9XR52
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.2.1 purified NiR exhibits a blue color spectrum showing maximum absorbance at 594 nm Fusarium oxysporum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.2.1 303
-
 pH 6.5, 35°C Fusarium oxysporum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.1 nitrite + methyl viologen
-
 Fusarium oxysporum NO + oxidized methyl viologen + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.2.1 ? x * 53705, calculated, x * 60000, SDS-PAGE Fusarium oxysporum

Synonyms

EC Number Synonyms Comment Organism
1.7.2.1 NirK
-
 Fusarium oxysporum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.2.1 6.5
-
 optimum below pH 6.5 Fusarium oxysporum