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Literature summary extracted from

  • Dubeau, M.; Ghinet, M.G.; Jacques, P.; Clermont, N.; Beaulieu, C.; Brzezinski, R.
    Cytosine deaminase as a negative selection marker for gene disruption and replacement in the genus Streptomyces and other actinobacteria (2009), Appl. Environ. Microbiol., 75, 1211-1214.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.5.4.1 pharmacology negative selection system for actinobacteria based on cytosine deaminase Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.1 pMP201 vector and derivatives with aminoglycoside resistance gene, synthetic version of the cytosine deaminase gene (CodA), optimized for expression in Streptomyces species, generation of Streptomyces lividans TK24 protoplasts, transformation of Streptomyces lividans Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
3.5.4.1 D314A PCR-directed mutagenesis, activity against 5-fluorocytosine 2-fold increased, 17-fold lower sensitivity against native cytosine Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.1 5-fluorocytosine + H2O Escherichia coli used as negative selection marker system, increased 5-fluorocytosine sensitivity in Streptomyces lividans 5-fluorouracil + NH3
-
 ?
3.5.4.1 cytosine + H2O Escherichia coli
-
 uracil + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.1 Escherichia coli P25524
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.1 additional information
-
 cytosine deaminase of Escherichia coli used as a negative selection marker in Streptomyces, can be applied for any actinobacterial species naturally resistant to 5-fluorocytosine while sensitive to 5-fluorouracil, valuable addition to the actinobacterial genetic toolbox Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.1 5-fluorocytosine + H2O used as negative selection marker system, increased 5-fluorocytosine sensitivity in Streptomyces lividans Escherichia coli 5-fluorouracil + NH3
-
 ?
3.5.4.1 cytosine + H2O
-
 Escherichia coli uracil + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.4.1 CodA protein
-
 Escherichia coli