EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.6 | cellobiose | enhanced activity with 4-nitrophenyl acetate as substrate | Trichoderma reesei | |
3.1.1.6 | D-galactose | enhanced activity with 4-nitrophenyl acetate as substrate | Trichoderma reesei | |
3.1.1.6 | D-glucose | 1% (w/v), enhanced activity with 4-nitrophenyl acetate as substrate | Trichoderma reesei | |
3.1.1.6 | D-xylose | at high concentrations of 10-500 mM, enhanced activity with 4-nitrophenyl acetate as substrate | Trichoderma reesei | |
3.1.1.6 | additional information | no influence on activity on 4-nitrophenyl acetate as substrate by L-arabinose, mannose, lactose | Trichoderma reesei | |
3.1.1.6 | additional information | rates of hydrolysis of 4-nitrophenyl acetate by the purified Aes1 are inhibited by low concentrations (1.0 to 5.0 mM) but significantly enhanced by high concentrations (10.0 to 500 mM) of D-xylose | Trichoderma reesei | |
3.1.1.6 | additional information | sodium acetate at concentrations below 100 mM does not inhibit the hydrolysis, higher concentrations inhibit the reactions | Trichoderma reesei | |
3.1.1.6 | additional information | using 4-nitrophenyl acetate as substrate the activity of the recombinant enzyme is enhanced by D-xylose, D-glucose, cellobiose, D-galactose, and xylooligosaccharides but not by arabinose, mannose, or lactose | Trichoderma reesei | |
3.1.1.6 | xylooligosaccharide | 50 mM, enhanced activity with 4-nitrophenyl acetate as substrate | Trichoderma reesei |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.6 | expression in Escherichia coli DH5 alpha | Trichoderma reesei |
3.1.1.6 | from genomic DNA in pT3C to generate expression plasmid pTSP-TAE and transformation of Hypocrea jecorina Rut-C30 | Trichoderma reesei |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.6 | D-xylose | at low concentrations of 1-5 mM, altered activity with 4-nitrophenyl acetate as substrate | Trichoderma reesei | |
3.1.1.6 | additional information | rates of hydrolysis of 4-nitrophenyl acetate by the purified Aes1 are inhibited by low concentrations (1.0 to 5.0 mM) but significantly enhanced by high concentrations (10.0 to 500 mM) of D-xylose | Trichoderma reesei | |
3.1.1.6 | Sodium acetate | at concentrations higher than 100 mM | Trichoderma reesei |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.6 | 0.23 | - |
4-nitrophenyl acetate | 30°C, pH 5.8 | Trichoderma reesei | |
3.1.1.6 | 0.23 | - |
4-nitrophenyl acetate | purified recombinant enzyme, 30°C, pH 5.8 | Trichoderma reesei |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.1.6 | extracellular | - |
Trichoderma reesei | - |
- |
3.1.1.6 | extracellular | 19 N-terminal residues serve as signal peptide for secretion | Trichoderma reesei | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.6 | 37090 | - |
theoretical, polypeptide of 348 amino acids | Trichoderma reesei |
3.1.1.6 | 42000 | - |
over-expression in Hypocrea jecorina Rut-C30, SDS-PAGE, molecular weight shift of purified recombinant enzyme (below theoretical weight) upon treatment with endoglycosidase H | Trichoderma reesei |
3.1.1.6 | 45000 | - |
SDS-PAGE | Trichoderma reesei |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.6 | additional information | Trichoderma reesei | new family of microbial esterases with hemicellulase activity for biodegradation of lignocellulose, complementary activity to acetylxylan esterase Axe1 | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.6 | Trichoderma reesei | A7J2C6 | anamorph Trichoderma reesei, strain Rut-C30 (hypercellulolytic mutant of Trichoderma reesei QM 6a) | - |
3.1.1.6 | Trichoderma reesei | A7J2C6 | Trichoderma reesei strain ATCC 56765 | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.1.1.6 | glycoprotein | N-glycosylation, sensitive to endoglycosidase H but not to O-glycosidase treatment, putative acceptor sites: N45, N146, N198, N204, N278 | Trichoderma reesei |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.6 | after over-expression (up to 20fold activity) by liquid column chromatography, monitored by activity measurements, 300 mg recombinant enzyme from 1 litre culture | Trichoderma reesei |
3.1.1.6 | centrifugation at 4°C, liquid column chromatography | Trichoderma reesei |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.1.6 | culture supernatant | - |
Trichoderma reesei | - |
3.1.1.6 | mycelium | high transcript level induced by 4 mM sophorose, 1% (w/v) cellulose, 1% (w/v) oat spelt xylan, or 1% (w/v) lactose, significant transcript levels induced by 1% (w/v) L-arabinose, low transcript levels induced by acetic acid (4 mM sodium acetate, 1% (w/v) glycerol), revealed by Northern blot | Trichoderma reesei | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.1.6 | additional information | - |
extremly low activities against 4-nitrophenyl formate, butyrate, or canoate | Trichoderma reesei |
3.1.1.6 | 0.000109 | - |
4-nitrophenyl butyrate, purified recombinant enzyme | Trichoderma reesei |
3.1.1.6 | 0.000213 | - |
4-nitrophenyl formate, purified recombinant enzyme | Trichoderma reesei |
3.1.1.6 | 0.21 | - |
4-nitrophenyl acetate, over-expression culture supernatant | Trichoderma reesei |
3.1.1.6 | 0.21 | - |
recombinant enzyme, culture supernatant | Trichoderma reesei |
3.1.1.6 | 0.85 | - |
recombinant enzyme, after CM Sepharose FF column | Trichoderma reesei |
3.1.1.6 | 1.12 | - |
recombinant enzyme, after DEAE FF column, 4-nitrophenyl acetate | Trichoderma reesei |
3.1.1.6 | 1.21 | - |
4-nitrophenyl acetate, purified recombinant enzyme | Trichoderma reesei |
3.1.1.6 | 20.5 | - |
4-nitrophenyl acetate, maximum velocity, purified recombinant enzyme, 30°C, pH 5.8 | Trichoderma reesei |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.6 | 4-nitrophenyl acetate + H2O | - |
Trichoderma reesei | 4-nitrophenol + acetate | - |
? | |
3.1.1.6 | 4-nitrophenyl acetate + H2O | 5 min, 30°C, pH 5.8, for kinetic analyses: 0.05-5 mM substrate in 100 mM sodium phosphate buffer | Trichoderma reesei | 4-nitrophenol + acetate | analysis by spectrophotometry at 410 nm | ? | |
3.1.1.6 | 4-nitrophenyl beta-D-xylopyranoside + H2O | 4 mM substrate, position-specificity, hydrolysis activity is specific for 3-, and 4-O-acetyl 4-nitrophenyl-beta-D-xylopyranoside, 2-O-acetyl 4-nitrophenyl-beta-D-xylopyranoside is a bad substrate | Trichoderma reesei | 4-nitrophenyl D-xylopyranose | beta-xylosidase-coupled assay | ? | |
3.1.1.6 | 4-nitrophenyl butyrate + H2O | 5 min, 30°C, pH 5.8 | Trichoderma reesei | 4-nitrophenol + butyrate | - |
? | |
3.1.1.6 | 4-nitrophenyl formate + H2O | 5 min, 30°C, pH 5.8 | Trichoderma reesei | 4-nitrophenol + formate | - |
? | |
3.1.1.6 | methyl-beta-D-xylopyranoside + vinylacetate | transacetylation of methylxyloside in aqueous solution (40°C, pH 6, 25 mM methyl-beta-D-xylopyranoside) | Trichoderma reesei | methyl-beta-D-xylopyranoside monoacetate + vinyl alcohol | analysis by thin-layer chromatography | ? | |
3.1.1.6 | additional information | new family of microbial esterases with hemicellulase activity for biodegradation of lignocellulose, complementary activity to acetylxylan esterase Axe1 | Trichoderma reesei | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.6 | Acetyl esterase | - |
Trichoderma reesei |
3.1.1.6 | Aes1 | - |
Trichoderma reesei |
3.1.1.6 | Aes1 | encoded by gene aes1, contains GDSY lipase motif | Trichoderma reesei |
3.1.1.6 | carbohydrate esterase | new family, less than 30% identity to carbohydrate esterases of known families | Trichoderma reesei |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.6 | additional information | - |
4-nitrophenyl-beta-D-xylopyranoside monoacetate | initial rate ratio between 2-, 3-, and 4-O-acetyl 4-nitrophenyl-beta-D-xylopyranoside is 1:19:17.7 | Trichoderma reesei |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.1.1.6 | Trichoderma reesei | theoretical | - |
5.9 |
3.1.1.6 | Trichoderma reesei | purified recombinant enzyme | - |
6 |