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Literature summary extracted from

  • Lambert, J.M.; Bongers, R.S.; de Vos, W.M.; Kleerebezem, M.
    Functional analysis of four bile salt hydrolase and penicillin acylase family members in Lactobacillus plantarum WCFS1 (2008), Appl. Environ. Microbiol., 74, 4719-4726.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.11
-
 Lactiplantibacillus plantarum
3.5.1.24 into pCR-Blunt and subsequently into pNZ8048 for heterologous overexpression of BSH in Lactococcus lactis cells Lactiplantibacillus plantarum WCFS1

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.11 Lactiplantibacillus plantarum
-
-
-
3.5.1.24 Lactiplantibacillus plantarum WCFS1 B9V401
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.24 glycochenodeoxycholic acid + H2O
-
 Lactiplantibacillus plantarum WCFS1 3alpha,7alpha-dihydroxy-5beta-cholanate + Gly
-
 ?
3.5.1.24 glycocholic acid + H2O
-
 Lactiplantibacillus plantarum WCFS1 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + Gly
-
 ?
3.5.1.24 glycodeoxycholic acid + H2O
-
 Lactiplantibacillus plantarum WCFS1 3alpha,12alpha-dihydroxy-5beta-cholanate + Gly
-
 ?
3.5.1.24 taurochenodeoxycholic acid + H2O
-
 Lactiplantibacillus plantarum WCFS1 chenodeoxycholate + taurine
-
 ?
3.5.1.24 taurocholic acid + H2O
-
 Lactiplantibacillus plantarum WCFS1 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
-
 ?
3.5.1.24 taurodeoxycholic acid + H2O
-
 Lactiplantibacillus plantarum WCFS1 3alpha,12alpha-dihydroxy-5beta-cholanate + taurine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.11 penicillin acylase Bsh3 and, to a lesser extent, Bsh2 and Bsh4 have a acylase activity of penicillin V, also activities toward penicillin G, ketocaproyl-homoserine lactone, and oxooctanoyl-homoserine lactone Lactiplantibacillus plantarum
3.5.1.24 bile salt hydrolase
-
 Lactiplantibacillus plantarum WCFS1
3.5.1.24 BSH1 computational analysis predicts four BSH-encoding genes, bsh1, bsh2, bsh3 and bsh4, BSH1 is found to be responsible for the majority of BSH activity Lactiplantibacillus plantarum WCFS1

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.24 37
-
 activity assay Lactiplantibacillus plantarum WCFS1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.24 6.5
-
 activity assay Lactiplantibacillus plantarum WCFS1