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Literature summary extracted from

  • Dong, J.; Tamaru, Y.; Araki, T.
    A unique beta-agarase, AgaA, from a marine bacterium, Vibrio sp. strain PO-303 (2007), Appl. Environ. Microbiol., 74, 1248-1255.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.81 ligated into a pET22b(+) vector and expressed in Escherichia coli BL21(DE3) competent cells Vibrio sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.81 AgNO3 partially inhibits the activity Vibrio sp.
3.2.1.81 AlCl3 completely inhibits the activity Vibrio sp.
3.2.1.81 CuCl2 completely inhibits the activity Vibrio sp.
3.2.1.81 CuSO4 completely inhibits the activity Vibrio sp.
3.2.1.81 FeCl3 partially inhibits the activity Vibrio sp.
3.2.1.81 HgCl2 completely inhibits the activity Vibrio sp.
3.2.1.81 MnCl2 partially inhibits the activity Vibrio sp.
3.2.1.81 additional information NaCl, KCl, MgCl2, and BaCl2 negligibly affect the enzymatic activity Vibrio sp.
3.2.1.81 Pb(CH3COO)2 completely inhibits the activity Vibrio sp.
3.2.1.81 SDS 0.1% SDS completely inhibits the activity Vibrio sp.
3.2.1.81 ZnCl2 completely inhibits the activity Vibrio sp.
3.2.1.81 ZnSO4 completely inhibits the activity Vibrio sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.81 CaCl2 slightly enhances enzymatic activity Vibrio sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.81 106100
-
 sequence analysis Vibrio sp.
3.2.1.81 120000
-
 1 * 120000, SDS-PAGE Vibrio sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.81 Vibrio sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.81 18.8fold and with a final yield of 16.8% by anion exchange and hydrophobic chromatographies Vibrio sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.81 0.872
-
 cell lysate Vibrio sp.
3.2.1.81 16.4
-
 18.8fold purified enzyme Vibrio sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.81 agar + H2O
-
 Vibrio sp. neoagarohexaose + neoagarotetraose
-
 ?
3.2.1.81 agarose + H2O
-
 Vibrio sp. neoagarohexaose + neoagarotetraose
-
 ?
3.2.1.81 additional information does not act on neoagarotetraose and neoagarobiose and kappa-carrageenan Vibrio sp. ?
-
 ?
3.2.1.81 neoagarohexaose + H2O
-
 Vibrio sp. ?
-
 ?
3.2.1.81 neoagarooctaose + H2O
-
 Vibrio sp. neoagarohexaose + neoagarotetraose + neoagarobiose
-
 ?
3.2.1.81 porphyran + H2O
-
 Vibrio sp. neoagarohexaose + neoagarotetraose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.81 monomer 1 * 120000, SDS-PAGE Vibrio sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.81 AgaA
-
 Vibrio sp.
3.2.1.81 beta-agarase
-
 Vibrio sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.81 40
-
-
 Vibrio sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.81 37
-
 stable under 37°C after a incubation of 10 min Vibrio sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.81 7.5
-
-
 Vibrio sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.81 5.5 11 after preincubated at various pHs at 25°C for 12 h Vibrio sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.81 Vibrio sp.
-
-
 4.14