Any feedback?
Literature summary extracted from
- Cloning and characterization of the xyl1 gene, encoding an NADH-preferring xylose reductase from Candida parapsilosis, and its functional expression in Candida tropicalis (2003), Appl. Environ. Microbiol., 69, 6179-6188.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.430 | 2-mercaptoethanol | 1 mM, increases activity by 13% | Candida parapsilosis |  |
| 1.1.1.430 | cysteine | 1 mM, increases activity by 29% | Candida parapsilosis | |
| 1.1.1.430 | dithiothreitol | 1 mM, increases activity by 43% | Candida parapsilosis | |
| 1.1.1.430 | glutathione | 1 mM, increases activity by 21% | Candida parapsilosis | |
| 1.1.1.430 | additional information | neither inhibited nor activated by EDTA at concentrations ranging from 1 to 10 mM | Candida parapsilosis |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.430 | expression in Candida tropicalis increases production of ethanol and glycerol | Candida parapsilosis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.430 | Cu2+ | activity is completely restored by addition of EDTA | Candida parapsilosis | |
| 1.1.1.430 | NAD+ | competitive with NADH, non-competitive with D-xylose | Candida parapsilosis | |
| 1.1.1.430 | xylitol | non-competitive against NADH and D-xylose | Candida parapsilosis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.430 | 0.0033 | - |
NADH | pH 6.0 | Candida parapsilosis | |
| 1.1.1.430 | 0.0365 | - |
NADPH | pH 6.0 | Candida parapsilosis | |
| 1.1.1.430 | 31.5 | - |
D-xylose | pH 6.0, coenzyme: NADH | Candida parapsilosis | |
| 1.1.1.430 | 151.7 | - |
D-erythrose | pH 6.0 | Candida parapsilosis | |
| 1.1.1.430 | 244.3 | - |
D-xylose | pH 6.0, coenzyme: NADPH | Candida parapsilosis | |
| 1.1.1.430 | 285.4 | - |
D-arabinose | pH 6.0 | Candida parapsilosis | |
| 1.1.1.430 | 302 | - |
D-ribose | pH 6.0 | Candida parapsilosis | |
| 1.1.1.430 | 326.3 | - |
xylitol | pH 6.0 | Candida parapsilosis | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.430 | CaCl2 | 1 mM, stimulates | Candida parapsilosis | |
| 1.1.1.430 | CoCl2 | 1 mM, stimulates | Candida parapsilosis | |
| 1.1.1.430 | FeCl2 | 1 mM, stimulates | Candida parapsilosis | |
| 1.1.1.430 | MgCl2 | 1 mM, stimulates | Candida parapsilosis | |
| 1.1.1.430 | MnCl2 | 1 mM, stimulates | Candida parapsilosis | |
| 1.1.1.430 | additional information | neither inhibited nor activated by EDTA at concentrations ranging from 1 to 10 mM | Candida parapsilosis | |
| 1.1.1.430 | NiCl2 | 1 mM, stimulates | Candida parapsilosis | |
| 1.1.1.430 | ZnCl2 | 1 mM, stimulates | Candida parapsilosis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.430 | 36400 | - |
2 * 36400, SDS-PAGE | Candida parapsilosis |
| 1.1.1.430 | 36629 | - |
2 * 36629, calculated from sequence | Candida parapsilosis |
| 1.1.1.430 | 69000 | - |
gel filtration | Candida parapsilosis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.430 | Candida parapsilosis | Q6Y0Z3 | KFCC-10875 | - |
| 1.1.1.430 | Candida parapsilosis KFCC-10875 | Q6Y0Z3 | KFCC-10875 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.430 | - |
Candida parapsilosis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.430 | 41.7 | - |
- |
Candida parapsilosis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.430 | D-arabinose + NADPH + H+ | - |
Candida parapsilosis | D-arabinitol + NADP+ | - |
? | |
| 1.1.1.430 | D-arabinose + NADPH + H+ | - |
Candida parapsilosis KFCC-10875 | D-arabinitol + NADP+ | - |
? | |
| 1.1.1.430 | D-erythrose + NADPH + H+ | - |
Candida parapsilosis | D-erythritol + NADP+ | - |
? | |
| 1.1.1.430 | D-erythrose + NADPH + H+ | - |
Candida parapsilosis KFCC-10875 | D-erythritol + NADP+ | - |
? | |
| 1.1.1.430 | D-ribose + NADPH + H+ | - |
Candida parapsilosis | ? | - |
? | |
| 1.1.1.430 | D-ribose + NADPH + H+ | - |
Candida parapsilosis KFCC-10875 | ? | - |
? | |
| 1.1.1.430 | D-xylose + NADH + H+ | the enzyme specifically transfers the 4-pro-R hydrogen from the C-4 of the nicotinamide ring to the re face of the carbonyl carbon of the substrate | Candida parapsilosis | xylitol + NAD+ | - |
r | |
| 1.1.1.430 | D-xylose + NADH + H+ | the enzyme specifically transfers the 4-pro-R hydrogen from the C-4 of the nicotinamide ring to the re face of the carbonyl carbon of the substrate | Candida parapsilosis KFCC-10875 | xylitol + NAD+ | - |
r | |
| 1.1.1.430 | D-xylose + NADPH + H+ | - |
Candida parapsilosis | xylitol + NADP+ | - |
? | |
| 1.1.1.430 | D-xylose + NADPH + H+ | - |
Candida parapsilosis KFCC-10875 | xylitol + NADP+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.430 | dimer | 2 * 36400, SDS-PAGE | Candida parapsilosis |
| 1.1.1.430 | dimer | 2 * 36629, calculated from sequence | Candida parapsilosis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.430 | XYL1 | - |
Candida parapsilosis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.430 | 37 | - |
assay at | Candida parapsilosis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.430 | 4 | - |
60 days, 50% loss of activity | Candida parapsilosis |
| 1.1.1.430 | 20 | - |
8 days, 50% loss of activity | Candida parapsilosis |
| 1.1.1.430 | 30 | - |
3 days, 50% loss of activity | Candida parapsilosis |
| 1.1.1.430 | 45 | - |
4.5 h, 50% loss of activity | Candida parapsilosis |
| 1.1.1.430 | 50 | - |
2 min, 50% loss of activity | Candida parapsilosis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.430 | 6 | - |
reduction of D-xylose | Candida parapsilosis |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.430 | 5 | 7 | pH 5.0: 81% of maximal activity, pH 7.0: about 65% of maximal activity | Candida parapsilosis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.430 | NADH | strongly prefers NADH to NADPH | Candida parapsilosis | |
| 1.1.1.430 | NADPH | strongly prefers NADH to NADPH | Candida parapsilosis | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.430 | 0.034 | - |
Cu2+ | pH 6.0 | Candida parapsilosis | |
| 1.1.1.430 | 0.18 | - |
NAD+ | pH 6.0, competitive with NADH | Candida parapsilosis | |
| 1.1.1.430 | 0.325 | - |
NAD+ | pH 6.0, non-competitive with D-xylose | Candida parapsilosis | |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 1.1.1.430 | Candida parapsilosis | calculated from sequence | - |
5.19 |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.430 | 0.019 | - |
D-xylose | pH 6.0, coenzyme: NADPH | Candida parapsilosis | |
| 1.1.1.430 | 0.028 | - |
D-arabinose | pH 6.0 | Candida parapsilosis | |
| 1.1.1.430 | 0.031 | - |
xylitol | pH 6.0 | Candida parapsilosis | |
| 1.1.1.430 | 0.043 | - |
D-ribose | pH 6.0 | Candida parapsilosis | |
| 1.1.1.430 | 0.089 | - |
D-erythrose | pH 6.0 | Candida parapsilosis | |
| 1.1.1.430 | 1.5 | - |
D-xylose | pH 6.0, coenzyme: NADH | Candida parapsilosis | |
| 1.1.1.430 | 127 | - |
NADPH | pH 6.0 | Candida parapsilosis | |
| 1.1.1.430 | 13900 | - |
NADH | pH 6.0 | Candida parapsilosis | |
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