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Literature summary extracted from

  • Yao, T.; Xu, L.; Ying, H.; Huang, H.; Yan, M.
    The catalytic property of 3-hydroxyisobutyrate dehydrogenase from Bacillus cereus on 3-hydroxypropionate (2009), Appl. Biochem. Biotechnol., 160, 694-703.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.31 additional information ethylene glycol tetraacetic acid or EDTA (all at 0.005 and 0.2 mM) do not affect 3-HIBADH activity Bacillus cereus

Application

EC Number Application Comment Organism
1.1.1.31 additional information 3-HIBADH may play a role in biosynthesis of 3-hydroxypropionate as a biological source Bacillus cereus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.31 expressed in Escherichia coli BL21 (DE3) Bacillus cereus
1.1.1.31 MmsB gene, overexpression in Escherichia coli strain BL21, subcloning in strain DH5alpha Bacillus cereus
1.1.1.31 pEB81 recombinant vector introduced into Escherichia coli BL21 to overexpress 3-HIBADH protein Bacillus cereus
1.1.1.59 expressed in Escherichia coli BL21 (DE3) Bacillus cereus
1.1.1.59 MmsB gene, overexpression in Escherichia coli strain BL21, subcloning in strain DH5alpha Bacillus cereus
1.1.1.298 expression in Escherichia coli strain BL21 Bacillus cereus
1.1.1.298 MmsB gene, overexpression in Escherichia coli strain BL21, subcloning in strain DH5alpha Bacillus cereus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.31 additional information is not inhibited by 1-propanol, lactate potassium, malate potassium, malonate potassium, acetaldehyde, ethanol, and glycerin Bacillus cereus
1.1.1.31 Zn2+ inhibits at 0.2 mM Bacillus cereus
1.1.1.31 ZnCl2 0.2 mM, 60% inhibition; inhibits approximately 60% of 3-HIBADH activity at 0.2 mM but shows no effect at 0.005 mM Bacillus cereus
1.1.1.59 ZnCl2 0.2 mM, 60% inhibition Bacillus cereus
1.1.1.298 additional information not inhibitory: EDTA and ethylene glykol, up to 0.2 mM Bacillus cereus
1.1.1.298 Zn 0.2 mM, 60% inhibition Bacillus cereus
1.1.1.298 Zn2+ 60% inhibition at 0.2 mM Bacillus cereus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.31 0.25
-
 NADP+
-
 Bacillus cereus
1.1.1.31 0.25
-
 NADP+ pH 8.5, 37°C Bacillus cereus
1.1.1.31 2.4
-
 NAD+
-
 Bacillus cereus
1.1.1.31 2.4
-
 NAD+ pH 8.5, 37°C Bacillus cereus
1.1.1.31 16.8
-
 3-hydroxypropionate
-
 Bacillus cereus
1.1.1.31 16.8
-
 3-hydroxypropionate pH 8.5, 37°C, KM-value for 3-hydroxyisobutyrate is approximately 20fold lower Bacillus cereus
1.1.1.59 additional information
-
 additional information steady-state kinetic analysis, overview Bacillus cereus
1.1.1.59 0.25
-
 NADP+ pH 8.5, 37°C Bacillus cereus
1.1.1.59 2.4
-
 NAD+ pH 8.5, 37°C Bacillus cereus
1.1.1.59 16.8
-
 3-hydroxypropanoate pH 8.5, 37°C Bacillus cereus
1.1.1.59 16.8
-
 3-hydroxypropionate pH 8.5, 37°C, KM-value for 3-hydroxyisobutyrate is approximately 20fold lower Bacillus cereus
1.1.1.298 additional information
-
 additional information steady-state kinetics, overview Bacillus cereus
1.1.1.298 additional information
-
 additional information steady-state kinetic analysis, overview Bacillus cereus
1.1.1.298 0.25
-
 NADP+ pH 8.5, 37°C Bacillus cereus
1.1.1.298 0.25
-
 NADP+ pH 8.5, 37°C, recombinant enzyme Bacillus cereus
1.1.1.298 2.4
-
 NAD+ pH 8.5, 37°C Bacillus cereus
1.1.1.298 2.4
-
 NAD+ pH 8.5, 37°C, recombinant enzyme Bacillus cereus
1.1.1.298 16.8
-
 3-hydroxypropionate pH 8.5, 37°C Bacillus cereus
1.1.1.298 16.8
-
 3-hydroxypropanoate pH 8.5, 37°C Bacillus cereus
1.1.1.298 16.8
-
 3-hydroxypropionate pH 8.5, 37°C, recombinant enzyme Bacillus cereus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.31 additional information no metal ion requirement Bacillus cereus
1.1.1.31 additional information has no metal ion requirement, MnSO4, CuSO4, CaCl2, MgSO4, and FeSO4 have no effect Bacillus cereus
1.1.1.59 additional information no metal ion requirement Bacillus cereus
1.1.1.298 additional information no metal ion requirement Bacillus cereus
1.1.1.298 additional information up to 0.2 mM, no effect on activity: MnSO4, CuSO4, CaCl2, MgSO4, and FeSO4 Bacillus cereus
1.1.1.298 additional information no metal ion requirement, no effects by 0.2 mM MnSO4, CuSO4, CaCl2, MgSO4, and FeSO4 Bacillus cereus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.31 32000
-
 gel filtration Bacillus cereus
1.1.1.59 32000
-
 x * 32000, SDS-PAGE Bacillus cereus
1.1.1.298 32000
-
 x * 32000, SDS-PAGE Bacillus cereus
1.1.1.298 32000
-
 x * 32000, recombinant enzyme, SDS-PAGE Bacillus cereus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.31 3-hydroxy-2-methylpropanoate + NAD+ Bacillus cereus
-
 2-methyl-3-oxopropanoate + NADH + H+
-
 r
1.1.1.31 3-hydroxy-2-methylpropanoate + NAD+ Bacillus cereus ATCC 14579
-
 2-methyl-3-oxopropanoate + NADH + H+
-
 r
1.1.1.31 3-hydroxy-2-methylpropanoate + NADP+ Bacillus cereus
-
 2-methyl-3-oxopropanoate + NADPH + H+
-
 r
1.1.1.31 3-hydroxy-2-methylpropanoate + NADP+ Bacillus cereus ATCC 14579
-
 2-methyl-3-oxopropanoate + NADPH + H+
-
 r
1.1.1.31 additional information Bacillus cereus MmsB from Bacillus cereus exhibits 3-hydroxyisobutyrate dehydrogenase as well as 3-hydroxypropionate dehydrogenase activity, EC 1.1.1.59 ?
-
 ?
1.1.1.31 additional information Bacillus cereus ATCC 14579 MmsB from Bacillus cereus exhibits 3-hydroxyisobutyrate dehydrogenase as well as 3-hydroxypropionate dehydrogenase activity, EC 1.1.1.59 ?
-
 ?
1.1.1.59 3-hydroxypropanoate + NAD+ Bacillus cereus
-
 3-oxopropanoate + NADH + H+
-
 r
1.1.1.59 additional information Bacillus cereus MmsB from Bacillus cereus exhibits 3-hydroxyisobutyrate dehydrogenase, EC 1.1.1.31, as well as 3-hydroxypropionate dehydrogenase activity ?
-
 ?
1.1.1.298 3-hydroxypropanoate + NADP+ Bacillus cereus
-
 3-oxopropanoate + NADPH + H+
-
 r
1.1.1.298 3-hydroxypropanoate + NADP+ Bacillus cereus
-
 malonate semialdehyde + NADPH + H+
-
 r
1.1.1.298 additional information Bacillus cereus MmsB from Bacillus cereus exhibits 3-hydroxyisobutyrate dehydrogenase, EC 1.1.1.31, as well as 3-hydroxypropionate dehydrogenase activity ?
-
 ?
1.1.1.298 additional information Bacillus cereus the enzyme is a 3-hydroxyisobutyrate dehydrogenase, 3-HIBADH, EC1.1.1.31, that also utilizes 3-hydroxypropionate as substrate. It catalyzes not only the oxidation of 3-hydroxyisobutyrate but also of L-serine, D-threonine, and other 3-hydroxyacid derivatives ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.31 Bacillus cereus
-
 ATCC 14579
-
1.1.1.31 Bacillus cereus
-
-
-
1.1.1.31 Bacillus cereus
-
 MmsB gene
-
1.1.1.31 Bacillus cereus ATCC 14579
-
-
-
1.1.1.31 Bacillus cereus ATCC 14579
-
 MmsB gene
-
1.1.1.59 Bacillus cereus
-
-
-
1.1.1.59 Bacillus cereus
-
 MmsB gene
-
1.1.1.59 Bacillus cereus ATCC 14579
-
-
-
1.1.1.59 Bacillus cereus ATCC 14579
-
 MmsB gene
-
1.1.1.298 Bacillus cereus
-
 gene MmsB
-
1.1.1.298 Bacillus cereus
-
-
-
1.1.1.298 Bacillus cereus
-
 MmsB gene
-
1.1.1.298 Bacillus cereus ATCC 14579
-
 gene MmsB
-
1.1.1.298 Bacillus cereus ATCC 14579
-
-
-
1.1.1.298 Bacillus cereus ATCC 14579
-
 MmsB gene
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.31 96fold purified at 4°C by ammonium sulfate fractionation and gel filtration Bacillus cereus
1.1.1.31 ammonium sulfate precipitation, hydrophobic interaction chromatography (Phenyl-Sepharose), anion exchange chromatography Bacillus cereus
1.1.1.31 native enzyme 100fold by ammonium sulfate fractionation, and hydrophobic interaction and anion exchange chromatography Bacillus cereus
1.1.1.59 ammonium sulfate precipitation, hydrophobic interaction chromatography (Phenyl-Sepharose), anion exchange chromatography Bacillus cereus
1.1.1.59 native enzyme 100fold by ammonium sulfate fractionation, and hydrophobic interaction and anion exchange chromatography Bacillus cereus
1.1.1.298 native enzyme 100fold by ammonium sulfate fractionation, and hydrophobic interaction and anion exchange chromatography Bacillus cereus
1.1.1.298 recombinant enzyme from Escherichia coli strain BL21 96fold by ammonium sulfate fractionation, hydrophobic interaction and anion exchange chromatography, followed by ultrafiltration Bacillus cereus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.31 0.091
-
 homogenate Bacillus cereus
1.1.1.31 8.7
-
 purified enzyme Bacillus cereus
1.1.1.31 8.77
-
 pH 8.5, 37°C Bacillus cereus
1.1.1.31 8.77
-
 purified enzyme, pH 8.5, 37°C Bacillus cereus
1.1.1.59 8.77
-
 pH 8.5, 37°C Bacillus cereus
1.1.1.298 8.7
-
 pH 8.5, 37°C Bacillus cereus
1.1.1.298 8.8
-
 purified recombinant enzyme, pH 8.5, 37°C Bacillus cereus

Storage Stability

EC Number Storage Stability Organism
1.1.1.31 -20°C, 50 mM Tris-HCl buffer, pH 8.5, 2 mM EDTA, 2 mM DTT, 50% glycerol Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.31 3-hydroxy-2-methylpropanoate + NAD+
-
 Bacillus cereus 2-methyl-3-oxopropanoate + NADH + H+
-
 r
1.1.1.31 3-hydroxy-2-methylpropanoate + NAD+
-
 Bacillus cereus ATCC 14579 2-methyl-3-oxopropanoate + NADH + H+
-
 r
1.1.1.31 3-hydroxy-2-methylpropanoate + NADP+
-
 Bacillus cereus 2-methyl-3-oxopropanoate + NADPH + H+
-
 r
1.1.1.31 3-hydroxy-2-methylpropanoate + NADP+
-
 Bacillus cereus ATCC 14579 2-methyl-3-oxopropanoate + NADPH + H+
-
 r
1.1.1.31 3-hydroxyisobutyrate + NAD+
-
 Bacillus cereus methylmalonate semialdehyde + NADH + H+
-
 r
1.1.1.31 3-hydroxypropanoate + NAD(P)+ is more active with NADP+ than NAD+ Bacillus cereus ?
-
 ?
1.1.1.31 3-hydroxypropanoate + NAD(P)+ is more active with NADP+ than NAD+ Bacillus cereus ATCC 14579 ?
-
 ?
1.1.1.31 3-hydroxypropanoate + NAD+
-
 Bacillus cereus malonate semialdehyde + NADH + H+
-
 r
1.1.1.31 3-hydroxypropanoate + NADP+
-
 Bacillus cereus malonate semialdehyde + NADPH + H+
-
 r
1.1.1.31 additional information MmsB from Bacillus cereus exhibits 3-hydroxyisobutyrate dehydrogenase as well as 3-hydroxypropionate dehydrogenase activity, EC 1.1.1.59 Bacillus cereus ?
-
 ?
1.1.1.31 additional information wide substrate specificity of the 3-HIBADH Bacillus cereus ?
-
 ?
1.1.1.31 additional information MmsB from Bacillus cereus exhibits 3-hydroxyisobutyrate dehydrogenase as well as 3-hydroxypropionate dehydrogenase activity, EC 1.1.1.59 Bacillus cereus ATCC 14579 ?
-
 ?
1.1.1.31 additional information wide substrate specificity of the 3-HIBADH Bacillus cereus ATCC 14579 ?
-
 ?
1.1.1.59 3-hydroxyisobutyrate + NAD+
-
 Bacillus cereus methylmalonate semialdehyde + NADH + H+
-
 r
1.1.1.59 3-hydroxypropanoate + NAD+
-
 Bacillus cereus 3-oxopropanoate + NADH + H+
-
 r
1.1.1.59 3-hydroxypropanoate + NAD+
-
 Bacillus cereus malonate semialdehyde + NADH + H+
-
 r
1.1.1.59 3-hydroxypropanoate + NADP+
-
 Bacillus cereus malonate semialdehyde + NADPH + H+
-
 r
1.1.1.59 additional information MmsB from Bacillus cereus exhibits 3-hydroxyisobutyrate dehydrogenase, EC 1.1.1.31, as well as 3-hydroxypropionate dehydrogenase activity Bacillus cereus ?
-
 ?
1.1.1.298 3-hydroxypropanoate + NAD+
-
 Bacillus cereus malonate semialdehyde + NADH + H+
-
 r
1.1.1.298 3-hydroxypropanoate + NAD+
-
 Bacillus cereus malonate-semialdehyde + NADH + H+
-
 ?
1.1.1.298 3-hydroxypropanoate + NADP+ 3-hydroxyisobutyrate dehydrogenase, EC 1.1.1.31, additionally exhibits 3-hydroxypropionate dehydrogenase activity Bacillus cereus malonate-semialdehyde + NADPH + H+
-
 ?
1.1.1.298 3-hydroxypropanoate + NADP+
-
 Bacillus cereus 3-oxopropanoate + NADPH + H+
-
 r
1.1.1.298 3-hydroxypropanoate + NADP+
-
 Bacillus cereus malonate semialdehyde + NADPH + H+
-
 r
1.1.1.298 3-hydroxypropanoate + NADP+
-
 Bacillus cereus malonate semialdehyde + NADPH + H+ spectrophotometric product determination r
1.1.1.298 additional information MmsB from Bacillus cereus exhibits 3-hydroxyisobutyrate dehydrogenase, EC 1.1.1.31, as well as 3-hydroxypropionate dehydrogenase activity Bacillus cereus ?
-
 ?
1.1.1.298 additional information the enzyme is a 3-hydroxyisobutyrate dehydrogenase, 3-HIBADH, EC1.1.1.31, that also utilizes 3-hydroxypropionate as substrate. It catalyzes not only the oxidation of 3-hydroxyisobutyrate but also of L-serine, D-threonine, and other 3-hydroxyacid derivatives Bacillus cereus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.31 ? x * 32000, SDS-PAGE Bacillus cereus
1.1.1.59 ? x * 32000, SDS-PAGE Bacillus cereus
1.1.1.298 ? x * 32000, SDS-PAGE Bacillus cereus
1.1.1.298 ? x * 32000, recombinant enzyme, SDS-PAGE Bacillus cereus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.31 3-HIBADH
-
 Bacillus cereus
1.1.1.31 3-hydroxyisobutyrate dehydrogenase cf. EC1.1.1.59, wide substrate specificity (hydroxyacid derivatives) Bacillus cereus
1.1.1.59 3-hydroxyisobutyrate dehydrogenase cf. EC1.1.1.31, wide substrate specificity (hydroxyacid derivatives) Bacillus cereus
1.1.1.298 3-HIBADH
-
 Bacillus cereus
1.1.1.298 3-hydroxyisobutyrate dehydrogenase
-
 Bacillus cereus
1.1.1.298 3-hydroxypropionate dehydrogenase
-
 Bacillus cereus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.31 37
-
-
 Bacillus cereus
1.1.1.31 37
-
 assay at Bacillus cereus
1.1.1.59 37
-
-
 Bacillus cereus
1.1.1.298 37
-
-
 Bacillus cereus
1.1.1.298 37
-
 assay at Bacillus cereus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.31 35 45 shows more than 90% of its optimized activity Bacillus cereus
1.1.1.59 35 45 90% of maximal activity within this range Bacillus cereus
1.1.1.298 35 45 90% of maximal activity within this range Bacillus cereus
1.1.1.298 35 45 activity range Bacillus cereus
1.1.1.298 35 45 more than 90% of maximum activity Bacillus cereus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.31 45
-
 enzyme activity is not decreased during preincubation at 45°C within 30 min, but preincubation at 55°C in 3 min can significantly denaturate and deactivate the enzyme Bacillus cereus
1.1.1.31 45
-
 stable at 45°C for 30 min, about 40% activity after 3 min at 55°C Bacillus cereus
1.1.1.59 45
-
 30 min, purified enzyme, completely stable Bacillus cereus
1.1.1.59 45
-
 stable at 45°C for 30 min, about 40% activity after 3 min at 55°C Bacillus cereus
1.1.1.59 55
-
 3 min, purified enzyme, significant denaturation and inactivation Bacillus cereus
1.1.1.298 37 45 purified enzyme, over 90% activity remaining after 30 min Bacillus cereus
1.1.1.298 45
-
 30 min, stable Bacillus cereus
1.1.1.298 45
-
 30 min, purified enzyme, completely stable Bacillus cereus
1.1.1.298 55
-
 3 min, about 40% residual activity Bacillus cereus
1.1.1.298 55
-
 3 min, purified enzyme, significant denaturation and inactivation Bacillus cereus
1.1.1.298 55
-
 purified enzyme, 30 min, partial denaturation Bacillus cereus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.31 0.21
-
 3-hydroxypropionate
-
 Bacillus cereus
1.1.1.31 0.21
-
 3-hydroxypropionate pH 8.5, 37°C Bacillus cereus
1.1.1.59 0.21
-
 3-hydroxypropionate pH 8.5, 37°C Bacillus cereus
1.1.1.59 0.21
-
 3-hydroxypropanoate pH 8.5, 37°C Bacillus cereus
1.1.1.298 0.21
-
 3-hydroxypropionate pH 8.5, 37°C Bacillus cereus
1.1.1.298 0.21
-
 3-hydroxypropanoate pH 8.5, 37°C Bacillus cereus
1.1.1.298 0.21
-
 3-hydroxypropionate pH 8.5, 37°C, recombinant enzyme Bacillus cereus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.31 8.5
-
 assay at Bacillus cereus
1.1.1.31 8.8 9
-
 Bacillus cereus
1.1.1.31 8.8 9 oxidation of 3-hydroxypropionate by 3-HIBADH Bacillus cereus
1.1.1.59 8.8 9
-
 Bacillus cereus
1.1.1.298 8.8
-
-
 Bacillus cereus
1.1.1.298 8.8 9
-
 Bacillus cereus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.31 7 10
-
 Bacillus cereus
1.1.1.31 7 10 oxidation of 3-hydroxypropionate by 3-HIBADH Bacillus cereus
1.1.1.59 7 10
-
 Bacillus cereus
1.1.1.59 7 10 activity range Bacillus cereus
1.1.1.298 7 10 activity range Bacillus cereus
1.1.1.298 8.2
-
 about 30% of maximum acticity Bacillus cereus
1.1.1.298 9
-
 about 65% of maximum activity Bacillus cereus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.31 additional information 3-HIBADH is more active with NADP+ than NAD+ Bacillus cereus
1.1.1.31 NAD(P)+ is more active with NADP+ than NAD+ Bacillus cereus
1.1.1.31 NAD+
-
 Bacillus cereus
1.1.1.31 NADH
-
 Bacillus cereus
1.1.1.31 NADP+
-
 Bacillus cereus
1.1.1.31 NADPH
-
 Bacillus cereus
1.1.1.59 NAD+
-
 Bacillus cereus
1.1.1.59 NADH
-
 Bacillus cereus
1.1.1.298 NAD+ NADP+ is preferred over NAD+ Bacillus cereus
1.1.1.298 NAD+ more active with NADP+ than NAD+ Bacillus cereus
1.1.1.298 NADH
-
 Bacillus cereus
1.1.1.298 NADP+
-
 Bacillus cereus
1.1.1.298 NADP+ NADP+ is preferred over NAD+ Bacillus cereus
1.1.1.298 NADP+ more active with NADP+ than NAD+ Bacillus cereus
1.1.1.298 NADPH
-
 Bacillus cereus

General Information

EC Number General Information Comment Organism
1.1.1.31 physiological function 3-hydroxyisobutyrate dehydrogenase is a key enzyme for the metabolism of valine and some keto-bodies. It can catalyze reversible conversion of 3-hydroxyisobutyrate to methylmalonate semialdehyde Bacillus cereus
1.1.1.298 physiological function the enzyme is a 3-hydroxyisobutyrate dehydrogenase, 3-HIBADH, EC1.1.1.31, that also utilizes 3-hydroxypropionate as substrate. It catalyzes not only the oxidation of 3-hydroxyisobutyrate but also of L-serine, D-threonine, and other 3-hydroxyacid derivatives. 3-HIBADH may have the similar function to 3-hydroxypropionate dehydrogenase in vivo and be the key enzyme in an autotrophic CO2 fixation pathway, the 3-hydroxypropionate cycle Bacillus cereus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.31 0.0125
-
 3-hydroxypropionate pH 8.5, 37°C Bacillus cereus
1.1.1.59 0.0125
-
 3-hydroxypropionate pH 8.5, 37°C Bacillus cereus
1.1.1.59 0.0125
-
 3-hydroxypropanoate pH 8.5, 37°C Bacillus cereus
1.1.1.298 0.0125
-
 3-hydroxypropionate pH 8.5, 37°C Bacillus cereus
1.1.1.298 0.0125
-
 3-hydroxypropanoate pH 8.5, 37°C Bacillus cereus
1.1.1.298 0.0125
-
 3-hydroxypropionate pH 8.5, 37°C, recombinant enzyme Bacillus cereus