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Literature summary extracted from

  • Yang, L.Q.; Wang, S.H.; Tian, Y.P.
    Purification, properties, and application of a novel acid urease from Enterobacter sp. (2010), Appl. Biochem. Biotechnol., 160, 303-313.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.5 food industry the enzyme is applicable to elimination of urea in Chinese rice wine Enterobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.5 0.0195
-
 Urea
-
 Enterobacter sp.
3.5.1.5 0.0195
-
 Urea 35°C, pH 4.5 Enterobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.5 72000
-
 6 * 72000, SDS-PAGE Enterobacter sp.
3.5.1.5 430000
-
 gel filtration Enterobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.5 Enterobacter sp.
-
-
-
3.5.1.5 Enterobacter sp.
-
 R-SYB082
-
3.5.1.5 Enterobacter sp. R-SYB082
-
-
-
3.5.1.5 Enterobacter sp. R-SYB082
-
 R-SYB082
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.5
-
 Enterobacter sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.5 101.7
-
-
 Enterobacter sp.
3.5.1.5 109
-
-
 Enterobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.5 urea + H2O
-
 Enterobacter sp. CO2 + NH3
-
 ?
3.5.1.5 urea + H2O
-
 Enterobacter sp. R-SYB082 CO2 + NH3
-
 ?
3.5.1.5 urea + H2O
-
 Enterobacter sp. CO2 + 2 NH3
-
 ?
3.5.1.5 urea + H2O
-
 Enterobacter sp. R-SYB082 CO2 + 2 NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.5 hexamer 6 * 72000, SDS-PAGE Enterobacter sp.
3.5.1.5 homohexamer 6 * 72000, SDS-PAGE Enterobacter sp.

Synonyms

EC Number Synonyms Comment Organism
3.5.1.5 urease
-
 Enterobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.5 35
-
-
 Enterobacter sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.5 25 75 25°C: about 80% of maximal activity, 75°C: about 45% of maximal activity Enterobacter sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.5 30
-
 30 min, stable Enterobacter sp.
3.5.1.5 50
-
 very stable below 50°C Enterobacter sp.
3.5.1.5 55
-
 30 min, 15% loss of activity Enterobacter sp.
3.5.1.5 60
-
 30 min, about 25% loss of activity Enterobacter sp.
3.5.1.5 70
-
 30 min, about 55% loss of activity Enterobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.5 4.5
-
-
 Enterobacter sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.5 3 7 pH 3.0: about 65% of maximal activity, pH 7.0: anout 75% of maximal activity Enterobacter sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.5 4 6
-
 Enterobacter sp.
3.5.1.5 4 6 35°C, 30 min, stable Enterobacter sp.