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Literature summary extracted from

  • Soerensen, H.P.; Madsen, L.S.; Petersen, J.; Andersen, J.T.; Hansen, A.M.; Beck, H.C.
    Oat (Avena sativa) seed extract as an antifungal food preservative through the catalytic activity of a highly abundant class I chitinase (2009), Appl. Biochem. Biotechnol., 160, 1573-1584.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 34000
-
 x * 34000, SDS-PAGE Hordeum vulgare
3.2.1.14 34000
-
 x * 34000, SDS-PAGE Secale cereale
3.2.1.14 34000
-
 x * 34000, SDS-PAGE Triticum aestivum
3.2.1.14 34000
-
 x * 34000, SDS-PAGE Avena sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.14 additional information Triticum aestivum the class I chitinase inhibits the growth of Penicillium roquefortii, overview ?
-
 ?
3.2.1.14 additional information Avena sativa the class I chitinase inhibits the growth of Penicillium roqueforti IBT 5426, overview ?
-
 ?
3.2.1.14 additional information Secale cereale the class I chitinase inhibits the growth of Penicillium roquefortii, overview ?
-
 ?
3.2.1.14 additional information Hordeum vulgare the class I chitinase inhibits the growth of Penicillium roquefortii, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Avena sativa P86181 cv. Adamo and Markant
-
3.2.1.14 Hordeum vulgare P11955 cv. Smilla
-
3.2.1.14 Hordeum vulgare Q42839 cv. Smilla
-
3.2.1.14 Secale cereale Q9AXR9
-
-
3.2.1.14 Secale cereale Q9FRV1
-
-
3.2.1.14 Triticum aestivum Q9XEN6 Cht4
-
3.2.1.14 Triticum aestivum Q6T484
-
-
3.2.1.14 Triticum aestivum Q8W428 Chi2
-
3.2.1.14 Triticum aestivum Q41539 Chi
-
3.2.1.14 Triticum aestivum Q8W427 Chi3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 native enzyme from seed extract by anion exchange and hydrophobic interaction exchange chromatography Avena sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.14 seed
-
 Triticum aestivum
-
3.2.1.14 seed
-
 Avena sativa
-
3.2.1.14 seed
-
 Secale cereale
-
3.2.1.14 seed
-
 Hordeum vulgare
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 additional information
-
 enzyme in oat seed extract Avena sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 additional information the class I chitinase inhibits the growth of Penicillium roquefortii, overview Triticum aestivum ?
-
 ?
3.2.1.14 additional information the class I chitinase inhibits the growth of Penicillium roqueforti IBT 5426, overview Avena sativa ?
-
 ?
3.2.1.14 additional information the class I chitinase inhibits the growth of Penicillium roquefortii, overview Secale cereale ?
-
 ?
3.2.1.14 additional information the class I chitinase inhibits the growth of Penicillium roquefortii, overview Hordeum vulgare ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 34000, SDS-PAGE Triticum aestivum
3.2.1.14 ? x * 34000, SDS-PAGE Avena sativa
3.2.1.14 ? x * 34000, SDS-PAGE Secale cereale
3.2.1.14 ? x * 34000, SDS-PAGE Hordeum vulgare
3.2.1.14 additional information determination of the primary structure by mass spectrometry, sequence comparisons, overview Triticum aestivum
3.2.1.14 additional information determination of the primary structure by mass spectrometry, sequence comparisons, overview Avena sativa
3.2.1.14 additional information determination of the primary structure by mass spectrometry, sequence comparisons, overview Secale cereale
3.2.1.14 additional information determination of the primary structure by mass spectrometry, sequence comparisons, overview Hordeum vulgare

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 CHI
-
 Triticum aestivum
3.2.1.14 CHI2
-
 Triticum aestivum
3.2.1.14 Chi3
-
 Triticum aestivum
3.2.1.14 Cht4
-
 Triticum aestivum
3.2.1.14 class I chitinase
-
 Triticum aestivum
3.2.1.14 class I chitinase
-
 Avena sativa
3.2.1.14 class I chitinase
-
 Secale cereale
3.2.1.14 class I chitinase
-
 Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 7
-
-
 Triticum aestivum
3.2.1.14 7
-
-
 Avena sativa
3.2.1.14 7
-
-
 Secale cereale
3.2.1.14 7
-
-
 Hordeum vulgare

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.14 Triticum aestivum two basic isoforms with pI values of 7.6 and 8.0
-
 7.6
3.2.1.14 Avena sativa two basic isoforms with pI values of 7.6 and 8.0
-
 7.6
3.2.1.14 Secale cereale two basic isoforms with pI values of 7.6 and 8.0
-
 7.6
3.2.1.14 Hordeum vulgare two basic isoforms with pI values of 7.6 and 8.0
-
 7.6
3.2.1.14 Triticum aestivum two basic isoforms with pI values of 7.6 and 8.0
-
 8
3.2.1.14 Avena sativa two basic isoforms with pI values of 7.6 and 8.0
-
 8
3.2.1.14 Secale cereale two basic isoforms with pI values of 7.6 and 8.0
-
 8
3.2.1.14 Hordeum vulgare two basic isoforms with pI values of 7.6 and 8.0
-
 8