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Literature summary extracted from

  • Yang, P.; Li, Y.; Wang, Y.; Meng, K.; Luo, H.; Yuan, T.; Bai, Y.; Zhan, Z.; Yao, B.
    A novel beta-mannanase with high specific activity from Bacillus circulans CGMCC1554: gene cloning, expression and enzymatic characterization (2008), Appl. Biochem. Biotechnol., 159, 85-94.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.78 expression in Pichia pastoris and Escherichia coli Niallia circulans

General Stability

EC Number General Stability Organism
3.2.1.78 enzyme is resistant to digestion by trypsin Niallia circulans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.78 Ag+ strong inhibition Niallia circulans
3.2.1.78 Hg2+ strong inhibition Niallia circulans
3.2.1.78 Sodium dodecyl sulfate strong inhibition Niallia circulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.78 32000
-
 x * 32000, calculated and SDS-PAGE Niallia circulans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.78 Niallia circulans Q0IJR6
-
-
3.2.1.78 Niallia circulans CGMCC1554 Q0IJR6
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.78 4839
-
 pH 7.6, 60°C Niallia circulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.78 locust bean gum + H2O
-
 Niallia circulans ? 76% of products are oligomannosides ?
3.2.1.78 locust bean gum + H2O
-
 Niallia circulans CGMCC1554 ? 76% of products are oligomannosides ?

Subunits

EC Number Subunits Comment Organism
3.2.1.78 ? x * 32000, calculated and SDS-PAGE Niallia circulans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.78 60
-
-
 Niallia circulans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.78 50
-
 60 min, 90% residual activity Niallia circulans
3.2.1.78 60
-
 20 min, complete loss of activity Niallia circulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.78 7.6
-
-
 Niallia circulans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.78 7 8 more than 75% of maximum activity within Niallia circulans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.78 6 10 retains more than 75% of maximum activity after 1 h incubation Niallia circulans