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Literature summary extracted from

  • Li, N.; Shi, P.; Yang, P.; Wang, Y.; Luo, H.; Bai, Y.; Zhou, Z.; Yao, B.
    Cloning, expression, and characterization of a new Streptomyces sp. S27 xylanase for which xylobiose is the main hydrolysis product (2008), Appl. Biochem. Biotechnol., 159, 521-531.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.8 beta-mercaptoethanol enhances the activity about 1.3- and 1.6fold at the concentrations of 1 and 10 mM, respectively Streptomyces sp. S27
3.2.1.8 additional information expression level of XynBS27 increases with induction time Streptomyces sp. S27

Application

EC Number Application Comment Organism
3.2.1.8 industry XynBS27 has several advantageous properties such as high specific activity, good activity over broad pH range, excellent pH stability, and being suitable for xylobiose production. These significant characteristics suggest that XynBS27 may be a good candidate in industrial application Streptomyces sp. S27

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 into the pPIC9 vector and overexpressed in Pichia pastoris GS115 Streptomyces sp. S27

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Ag+ strongly inhibits by 69.1% even at the concentration of 1 mM Streptomyces sp. S27
3.2.1.8 Ca2+ 10 mM reduces the enzyme activity by 9.7% Streptomyces sp. S27
3.2.1.8 Co2+ 10 mM reduces the enzyme activity by 26.1% Streptomyces sp. S27
3.2.1.8 Cu2+ 10 mM reduces the enzyme activity by 49.6% Streptomyces sp. S27
3.2.1.8 Fe2+ 10 mM reduces the enzyme activity by 31.0% Streptomyces sp. S27
3.2.1.8 Fe3+ 10 mM reduces the enzyme activity by 11.3% Streptomyces sp. S27
3.2.1.8 Hg2+ 1 mM completely inhibits Streptomyces sp. S27
3.2.1.8 K+ 10 mM reduces the enzyme activity by 11.1% Streptomyces sp. S27
3.2.1.8 Li+ 10 mM reduces the enzyme activity by 16.1% Streptomyces sp. S27
3.2.1.8 additional information EDTA, Ni2+, Zn2+ and Mg2+ have no effect on activity Streptomyces sp. S27
3.2.1.8 Na+ 10 mM reduces the enzyme activity by 9.8% Streptomyces sp. S27
3.2.1.8 Pb2+ 10 mM reduces the enzyme activity by 32.9% Streptomyces sp. S27
3.2.1.8 SDS 10 mM reduces the enzyme activity by 59.2% Streptomyces sp. S27

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.8 Cr3+ 10 mM enhances the enzyme activity by 16.3% Streptomyces sp. S27

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 21000
-
 sequence analysis Streptomyces sp. S27

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Streptomyces sp. S27
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 recombinant XynBS27 purified to electrophoretic homogeneity by anion exchange chromatography Streptomyces sp. S27

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 3272
-
 purified enzyme Streptomyces sp. S27

Storage Stability

EC Number Storage Stability Organism
3.2.1.8 4°C Streptomyces sp. S27

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 birchwood xylan + H2O main hydrolysis product of xylan is xylobiose (more than 75%) Streptomyces sp. S27 D-xylose + xylobiose + xylotriose + ?
-
 ?
3.2.1.8 oat spelt xylan + H2O main hydrolysis product of xylan is xylobiose (more than 75%) Streptomyces sp. S27 xylobiose + D-xylose + xylotriose + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 xylanase
-
 Streptomyces sp. S27
3.2.1.8 XynBS27
-
 Streptomyces sp. S27

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 65
-
-
 Streptomyces sp. S27

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.8 1737
-
 birchwood xylan
-
 Streptomyces sp. S27
3.2.1.8 3475
-
 oat spelt xylan
-
 Streptomyces sp. S27

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 6.5
-
-
 Streptomyces sp. S27

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.8 4 12 XynBS27 shows good pH stability and retains more than 80% of the maximum activity after incubation at 37°C for 1 h. Between 5-8 retains over 65% of its activity Streptomyces sp. S27