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Literature summary extracted from

  • Yadav, S.; Yadav, P.K.; Yadav, D.; Yadav, K.D.
    Purification and characterization of pectin lyase produced by Aspergillus terricola and its application in retting of natural fibers (2008), Appl. Biochem. Biotechnol., 159, 270-283.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.2.10 Sodium arsenate slightly promotes the activity of PNL Aspergillus terricola

Application

EC Number Application Comment Organism
4.2.2.10 additional information the purified pectin lyase shows retting ability for natural fibers viz. Cannabis sativa and Linum usitatissimum Aspergillus terricola

General Stability

EC Number General Stability Organism
4.2.2.10 the purified enzyme retains its full activity after exposure for 1 h at 60°C in 1.0 M ammonium sulfate, in the absence of ammonium sulfate the enzyme loses its 77% activity at 60°C, at higher temperatures (70-100°C), ammonium sulfate is not able to stabilize the activity of the pectin lyase Aspergillus terricola
4.2.2.10 the purified enzyme retains its full activity after exposure for 1 h at 60°C in 1.0 M ammonium sulfate, in the absence of ammonium sulfate, the enzyme loses its 77% activity at 60°C, at higher temperatures (70-100°C), ammonium sulfate is not able to stabilize the activity of the pectin lyase Aspergillus terricola

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.10 Ag+ complete inhibition at 1 mM Aspergillus terricola
4.2.2.10 Cu2+ 10% residual activity at 1 mM Aspergillus terricola
4.2.2.10 EDTA 85% residual activity at 1 mM Aspergillus terricola
4.2.2.10 Hg2+ 24% residual activity at 1 mM Aspergillus terricola
4.2.2.10 K+ 91% residual activity at 1 mM; 92% residual activity at 1 mM Aspergillus terricola
4.2.2.10 K4[Fe(CN)6] complete inhibition at 1 mM Aspergillus terricola
4.2.2.10 KMnO4 complete inhibition at 1 mM Aspergillus terricola
4.2.2.10 Mg2+ 97% residual activity at 1 mM Aspergillus terricola
4.2.2.10 Mn2+ 93% residual activity at 1 mM Aspergillus terricola
4.2.2.10 potassium ferrocyanide complete inhibition at 1 mM Aspergillus terricola
4.2.2.10 potassium permanganate complete inhibition at 1 mM Aspergillus terricola
4.2.2.10 Sodium azide 79% residual activity at 1 mM Aspergillus terricola
4.2.2.10 Zn2+ 69% residual activity at 1 mM Aspergillus terricola

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.10 Ca2+ slightly promotes the activity of PNL (105% relative activity at 1 mM) Aspergillus terricola
4.2.2.10 Ca2+ 105% relative activity at 1 mM Aspergillus terricola
4.2.2.10 Co2+ slightly promotes the activity of PNL (114% relative activity at 1 mM) Aspergillus terricola
4.2.2.10 Co2+ 114% relative activity at 1 mM Aspergillus terricola
4.2.2.10 Na+ slightly promotes the activity of PNL (108.3% relative activity at 1 mM) Aspergillus terricola
4.2.2.10 Sodium arsenate slightly promotes the activity of PNL (110% relative activity at 1 mM) Aspergillus terricola

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.10 35000
-
 SDS-PAGE Aspergillus terricola

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.10 Aspergillus terricola
-
-
-
4.2.2.10 Aspergillus terricola MTCC 7588
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.10 DEAE cellulose column chromatography and Sephadex G-100 gel filtration Aspergillus terricola
4.2.2.10 DEAE-cellulose column chromatography and Sephadex G100 gel filtration Aspergillus terricola

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.10 citrus pectin
-
 Aspergillus terricola ?
-
 ?
4.2.2.10 citrus pectin
-
 Aspergillus terricola MTCC 7588 ?
-
 ?
4.2.2.10 additional information pectin lyase shows retting ability for natural fibers of Cannabis sativa and Linum usitatissimum Aspergillus terricola ?
-
 ?
4.2.2.10 additional information pectin lyase shows retting ability for natural fibers of Cannabis sativa and Linum usitatissimum Aspergillus terricola MTCC 7588 ?
-
 ?
4.2.2.10 pectin
-
 Aspergillus terricola ?
-
 ?
4.2.2.10 pectin
-
 Aspergillus terricola MTCC 7588 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.2.10 PNL
-
 Aspergillus terricola

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.10 50
-
-
 Aspergillus terricola

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.10 50 60 the enzyme retains its full activity up to 50°C, and thereafter, activity decreases with increase in temperature, 75% of the activity is lost after incubating the purified PNL at 60°C for 1 h, the half-lives at 60, 70, 80, and 85°C are 7.03, 3.9, 1.8, and 0.89 min, respectively Aspergillus terricola
4.2.2.10 50 60 the enzyme retains its full activity up to 50°C, and thereafter, activity decreased with increase in temperature, however 75% of the activity is lost after incubating the purified PNL at 60°C for 1 h Aspergillus terricola

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.2.10 110
-
 citrus pectin at 37°C Aspergillus terricola
4.2.2.10 110
-
 Pectin purified enzyme, at 37°C Aspergillus terricola

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.10 8
-
-
 Aspergillus terricola

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.10 4 9
-
 Aspergillus terricola
4.2.2.10 4 9 the enzyme retains approximately 100% of its maximum activity for 24 h in a buffer of pH range 4.0-9.0 Aspergillus terricola