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Literature summary extracted from

  • Tovar-Castro, L.; Garcia-Garibay, M.; Saucedo-Castaneda, G.
    Lactase production by solid-state cultivation of Kluyveromyces marxianus CDBBL 278 on an inert support: effect of inoculum, buffer, and nitrogen source (2008), Appl. Biochem. Biotechnol., 151, 610-617.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.23 industry assimilation of lactose as a by-product of dairy industry Kluyveromyces marxianus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.7 extracellular
-
 Kluyveromyces marxianus
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.7 Kluyveromyces marxianus
-
 CDBBL 278
-
3.2.1.7 Kluyveromyces marxianus CDBBL 278
-
 CDBBL 278
-
3.2.1.23 Kluyveromyces marxianus
-
-
-
3.2.1.23 Kluyveromyces marxianus CDBBL 278
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.7 0.0107
-
 60% of nitrogen source for yeast is supplemented as urea Kluyveromyces marxianus
3.2.1.7 0.0692
-
 40% of nitrogen source for yeast is supplemented as urea Kluyveromyces marxianus
3.2.1.7 0.2031
-
 nitrogen source for yeast is ammonium sulfate Kluyveromyces marxianus
3.2.1.7 0.2416
-
 37°C, 15 min, pH 4,5, 20% of nitrogen source for yeast is supplemented as urea Kluyveromyces marxianus
3.2.1.23 0.3346
-
 40% of nitrogen source for yeast is supplemented as urea Kluyveromyces marxianus
3.2.1.23 0.6597
-
 nitrogen source for yeast is ammonium sulfate Kluyveromyces marxianus
3.2.1.23 1.148
-
 37°C, 3 min, pH 6.6, 20% of nitrogen source for yeast is supplemented as urea Kluyveromyces marxianus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.7 inulin + H2O 37°C, 15 min, pH 4.5 Kluyveromyces marxianus ?
-
 ?
3.2.1.7 inulin + H2O 37°C, 15 min, pH 4.5 Kluyveromyces marxianus CDBBL 278 ?
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O pH 6.3, 3 min, 47°C Kluyveromyces marxianus 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O pH 6.3, 3 min, 47°C Kluyveromyces marxianus CDBBL 278 2-nitrophenol + beta-D-galactose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.7 Insulinase
-
 Kluyveromyces marxianus
3.2.1.23 beta-galactosidase
-
 Kluyveromyces marxianus
3.2.1.23 lactase
-
 Kluyveromyces marxianus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.7 37
-
 pH 4.5 Kluyveromyces marxianus
3.2.1.23 37
-
 pH 6.6 Kluyveromyces marxianus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.7 4.5
-
 37°C Kluyveromyces marxianus
3.2.1.23 6.6
-
 37°C Kluyveromyces marxianus