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Literature summary extracted from

  • Apiraksakorn, J.; Nitisinprasert, S.; Levin, R.E.
    Grass degrading beta-1,3-1,4-D-glucanases from Bacillus subtilis GN156: purification and characterization of glucanase J1 and pJ2 possessing extremely acidic pI (2008), Appl. Biochem. Biotechnol., 149, 53-66.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.73 Ca2+ partially inhibited Bacillus subtilis
3.2.1.73 Cu2+ partially inhibited Bacillus subtilis
3.2.1.73 Fe2+ slightly inhibited Bacillus subtilis
3.2.1.73 Mg2+ slightly inhibited Bacillus subtilis
3.2.1.73 Mn2+ partially inhibited Bacillus subtilis
3.2.1.73 additional information not inhibited by Li+; not inhibited by Li+, not significantly by Fe2+ Bacillus subtilis
3.2.1.73 Zn2+ partially inhibited Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.73 additional information
-
 Barley beta-glucan 1.53 mg/ml Bacillus subtilis
3.2.1.73 additional information
-
 Barley beta-glucan 4.36 mg/ml with substrate barley beta-glucan Bacillus subtilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.73 extracellular
-
 Bacillus subtilis
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.73 25000
-
 SDS-PAGE Bacillus subtilis
3.2.1.73 90000
-
 SDS-PAGE, J2 is composed of three protein subunits of 40, 32 and 18 kDa Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.73 Bacillus subtilis
-
-
-
3.2.1.73 Bacillus subtilis GN156
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.73 affinity chromatography of epoxy-activated sepharose 6B and ultrafiltration technique Bacillus subtilis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.73 culture supernatant
-
 Bacillus subtilis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.73 82.45
-
-
 Bacillus subtilis
3.2.1.73 555.1
-
 purified enzyme Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.73 barley beta-glucan + H2O
-
 Bacillus subtilis additional information pentose, triose, tetarose and a high molecular weight polysaccharide with 1,3 linkage ?
3.2.1.73 barley beta-glucan + H2O
-
 Bacillus subtilis additional information pentose, triose, tetrose and a high molecular weight polysaccharide with 1,3 linkage ?
3.2.1.73 barley beta-glucan + H2O
-
 Bacillus subtilis GN156 additional information pentose, triose, tetarose and a high molecular weight polysaccharide with 1,3 linkage ?
3.2.1.73 barley beta-glucan + H2O
-
 Bacillus subtilis GN156 additional information pentose, triose, tetrose and a high molecular weight polysaccharide with 1,3 linkage ?
3.2.1.73 additional information no activity against carboxymethylcellulose, xylan, laminarin, dextrin Bacillus subtilis ?
-
 ?
3.2.1.73 additional information no activity against carboxymethylcellulose, xylan, laminarin, dextrin Bacillus subtilis GN156 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.73 More four protein bands of approximately 40, 32, 25 and 18 kDa are resolved from pJ2 Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.73 1,3-1,4-beta-D-glucan-4-glucano hydrolase
-
 Bacillus subtilis
3.2.1.73 beta-1,3-1,4-glucanase
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.73 60
-
-
 Bacillus subtilis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.73 20 70 30 min with about 50% of activity remaining at 50°C Bacillus subtilis
3.2.1.73 20 70 the remaining activity decreased to 20% at 20-40°C and is completely destroyed at 50-70°C Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.73 20 50 30 min with about 50% of activity remaining at 50°C Bacillus subtilis
3.2.1.73 20 40 the remaining activity decreased to 20% at 20-40°C and is completely destroyed at 50-70°C Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.73 0.074
-
 Barley beta-glucan
-
 Bacillus subtilis
3.2.1.73 0.091
-
 Barley beta-glucan
-
 Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.73 6
-
-
 Bacillus subtilis
3.2.1.73 6.5
-
-
 Bacillus subtilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.73 6 9 with 50% of the activity remaining at pH 9 Bacillus subtilis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.73 6 9 with 50% of the activity remaining at pH 9 Bacillus subtilis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.73 Bacillus subtilis isoelectric focusing
-
 3.5
3.2.1.73 Bacillus subtilis isoelectricfocusing
-
 3.6