EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.22.33 | Cu2+ | inhibits casein degradation, complete inhibition | Ananas comosus | |
3.4.22.33 | Hg2+ | inhibits casein degradation, complete inhibition | Ananas comosus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.22.33 | additional information | poor or no inhibition by ions K+, Ca2+, Mg2+, Mn2+, Co2+, Bo3+, Al3+, Cr2+, Ni2+, Sn2+, Ag+, Fe2+, Cd2+, Zn2+, W3+, Se2+, As3+, and Pb2+, overview | Ananas comosus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.22.33 | Ananas comosus | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.22.33 | commercial preparation | - |
Ananas comosus | - |
3.4.22.33 | fruit | bromelain is a mixture of bromelain A and B, the proteolytic enzymes of pineapple fruit | Ananas comosus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.22.33 | azocasein + H2O | - |
Ananas comosus | ? | - |
? | |
3.4.22.33 | benzoyl-L-arginine ethyl ester + H2O | artificial substrate | Ananas comosus | ? | - |
? | |
3.4.22.33 | casein + H2O | enzyme is assayed using casein as a substrate with Coomassie dye to track completion of hydrolysis of casein, casein is hydrolysed to completion leading to a brown coloured solution, assay method development as a Bradford dye-binding-bromelain-casein system as an assay for heavy metals, based on colour development and its inhibition by Hg2+ and Cu2+ | Ananas comosus | ? | - |
? | |
3.4.22.33 | additional information | bromelain is a cysteine protease containing a single thiol group in the active sites | Ananas comosus | ? | - |
? | |
3.4.22.33 | N-benzoyl-L-tyrosine ethyl ester + H2O | artificial substrate | Ananas comosus | ? | - |
? | |
3.4.22.33 | Nalpha-benzoyl-L-arginine-4-nitroanilide + H2O | artificial substrate | Ananas comosus | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.22.33 | bromelain A | - |
Ananas comosus |
3.4.22.33 | bromelain B | - |
Ananas comosus |