Literature summary extracted from

Zhang, T.; Gong, F.; Chi, Z.; Liu, G.; Chi, Z.; Sheng, J.; Li, J.; Wang, X.
Cloning and characterization of the inulinase gene from a marine yeast Pichia guilliermondii and its expression in Pichia pastoris (2009), Antonie van Leeuwenhoek, 95, 13-22.

Application

EC Number	Application	Comment	Organism
3.2.1.7	additional information	inulinase from Pichia guilliermondii strain 1 is closely related to that from Kluyveromyces marxianus	Meyerozyma guilliermondii

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.2.1.7	inulinase gene without the signal sequence subcloned into pPICZaA expression vector and expressed in Pichia pastoris X-33	Meyerozyma guilliermondii

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
3.2.1.7	60000	-	Western blot analysis, His-tagged fusion protein of the recombinant inulinase	Meyerozyma guilliermondii

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.2.1.7	Meyerozyma guilliermondii	A5DHM6	-	-
3.2.1.7	Meyerozyma guilliermondii 1	A5DHM6	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.2.1.7	inulin + H2O	hydrolysis with the crude recombinant inulinase	Meyerozyma guilliermondii	monosaccharide + disaccharide + oligosaccharide	-	?
3.2.1.7	inulin + H2O	hydrolysis with the crude recombinant inulinase	Meyerozyma guilliermondii 1	monosaccharide + disaccharide + oligosaccharide	-	?

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Release 2023.1 (January 2023)