EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.2.6 | cloned into pBC-SK and expressed in Escherichia coli HB101 | Klebsiella aerogenes |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.5.2.6 | E104K | side directed mutagenesis | Klebsiella aerogenes |
3.5.2.6 | E240V | side directed mutagenesis | Klebsiella aerogenes |
3.5.2.6 | E240V | substitution increases catalytic efficiency toward ceftazidime | Klebsiella aerogenes |
3.5.2.6 | M182T | side directed mutagenesis | Klebsiella aerogenes |
3.5.2.6 | R164S | side directed mutagenesis | Klebsiella aerogenes |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.2.6 | clavulanic acid | - |
Klebsiella aerogenes | |
3.5.2.6 | Sulbactam | - |
Klebsiella aerogenes | |
3.5.2.6 | tazobactam | - |
Klebsiella aerogenes |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.2.6 | 0.0006 | - |
benzylpenicillin | 100 microM nitrocefin as the reporter substrate, TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes | |
3.5.2.6 | 0.0007 | - |
benzylpenicillin | 100 microM nitrocefin as the reporter substrate, TEM-149 mutant T182M, pH 7.0, 25°C | Klebsiella aerogenes | |
3.5.2.6 | 0.0014 | - |
piperacillin | 100 microM nitrocefin as the reporter substrate, TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes | |
3.5.2.6 | 0.0034 | - |
piperacillin | 100 microM nitrocefin as the reporter substrate, TEM-149 mutant T182M, pH 7.0, 25°C | Klebsiella aerogenes | |
3.5.2.6 | 0.005 | - |
ampicillin | 100 microM nitrocefin as the reporter substrate, TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes | |
3.5.2.6 | 0.016 | - |
cefepime | TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes | |
3.5.2.6 | 0.019 | - |
ampicillin | 100 microM nitrocefin as the reporter substrate, TEM-149 mutant T182M, pH 7.0, 25°C | Klebsiella aerogenes | |
3.5.2.6 | 0.019 | - |
ceftazidime | TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes | |
3.5.2.6 | 0.02 | - |
nitrocefin | TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes | |
3.5.2.6 | 0.025 | - |
nitrocefin | TEM-149 mutant T182M, pH 7.0, 25°C | Klebsiella aerogenes | |
3.5.2.6 | 0.026 | - |
ceftazidime | TEM-149 mutant T182M, pH 7.0, 25°C | Klebsiella aerogenes | |
3.5.2.6 | 0.027 | - |
aztreonam | TEM-149 mutant T182M, pH 7.0, 25°C | Klebsiella aerogenes | |
3.5.2.6 | 0.03 | - |
cefepime | TEM-149 mutant T182M, pH 7.0, 25°C | Klebsiella aerogenes | |
3.5.2.6 | 0.032 | - |
cefotaxime | TEM-149 mutant T182M, pH 7.0, 25°C | Klebsiella aerogenes | |
3.5.2.6 | 0.035 | - |
cefazolin | TEM-149 mutant T182M, pH 7.0, 25°C | Klebsiella aerogenes | |
3.5.2.6 | 0.043 | - |
cefotaxime | TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes | |
3.5.2.6 | 0.051 | - |
aztreonam | TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes | |
3.5.2.6 | 0.068 | - |
cefazolin | TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.2.6 | 28600 | - |
- |
Klebsiella aerogenes |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.2.6 | Klebsiella aerogenes | Q1KY81 | SS-13 | - |
3.5.2.6 | Klebsiella aerogenes SS-13 | Q1KY81 | SS-13 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.2.6 | - |
Klebsiella aerogenes |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.5.2.6 | additional information | isolated from Homo sapiens urine | Klebsiella aerogenes | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.2.6 | ampicillin + H2O | - |
Klebsiella aerogenes | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
3.5.2.6 | ampicillin + H2O | - |
Klebsiella aerogenes SS-13 | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
3.5.2.6 | aztreonam + H2O | - |
Klebsiella aerogenes | [(1S,2S)-1-[[(2Z)-2-(2-ammonio-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino]-1-carboxypropan-2-yl]sulfamate | - |
? | |
3.5.2.6 | aztreonam + H2O | - |
Klebsiella aerogenes SS-13 | [(1S,2S)-1-[[(2Z)-2-(2-ammonio-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino]-1-carboxypropan-2-yl]sulfamate | - |
? | |
3.5.2.6 | benzylpenicillin + H2O | - |
Klebsiella aerogenes | (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
3.5.2.6 | benzylpenicillin + H2O | - |
Klebsiella aerogenes SS-13 | (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
3.5.2.6 | cefazolin + H2O | - |
Klebsiella aerogenes | (2R)-2-[(R)-carboxy[(1H-tetrazol-1-ylacetyl)amino]methyl]-5-[[(5-methyl-1,3,4-thiadiazol-2-yl)sulfanyl]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
3.5.2.6 | cefazolin + H2O | - |
Klebsiella aerogenes SS-13 | (2R)-2-[(R)-carboxy[(1H-tetrazol-1-ylacetyl)amino]methyl]-5-[[(5-methyl-1,3,4-thiadiazol-2-yl)sulfanyl]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
3.5.2.6 | cefepime + H2O | - |
Klebsiella aerogenes | (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[(1-methylpyrrolidinium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
3.5.2.6 | cefotaxime + H2O | - |
Klebsiella aerogenes | (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
3.5.2.6 | cefotaxime + H2O | - |
Klebsiella aerogenes SS-13 | (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
3.5.2.6 | ceftazidime + H2O | preferred and best substrate | Klebsiella aerogenes | (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
3.5.2.6 | nitrocefin + H2O | - |
Klebsiella aerogenes | (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
3.5.2.6 | piperacillin + H2O | - |
Klebsiella aerogenes | (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.2.6 | beta-lactamase | - |
Klebsiella aerogenes |
3.5.2.6 | TEM-149 | - |
Klebsiella aerogenes |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.2.6 | 25 | 55 | thermal stability of TEM-149 wild-type and TEM-149T182M mutant is determined by measuring the residual activity after incubation of the enzymes at three different temperatures: 25°C, 40°C, and 55°C. TEM-149 wild-type exhibits half-lives of 90 min at 25°C, 70 min at 40°C, and 30 min at 55°C, whereas TEM-149T182M mutant shows half-lives greater than 120 min at 25°C and 40°C and 45 min at 55°C. Compared to the thermal stability of TEM-149, the thermal stability of the TEM-149T182M mutant is substantially increased | Klebsiella aerogenes |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.2.6 | 0.07 | - |
cefotaxime | TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes | |
3.5.2.6 | 0.41 | - |
cefotaxime | TEM-149 mutant T182M, pH 7.0, 25°C | Klebsiella aerogenes | |
3.5.2.6 | 0.43 | - |
cefepime | TEM-149 mutant T182M, pH 7.0, 25°C | Klebsiella aerogenes | |
3.5.2.6 | 0.56 | - |
cefazolin | TEM-149 mutant T182M, pH 7.0, 25°C | Klebsiella aerogenes | |
3.5.2.6 | 2.2 | - |
cefazolin | TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes | |
3.5.2.6 | 2.4 | - |
ampicillin | TEM-149 mutant T182M, pH 7.0, 25°C | Klebsiella aerogenes | |
3.5.2.6 | 2.4 | - |
cefepime | TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes | |
3.5.2.6 | 2.7 | - |
piperacillin | TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes | |
3.5.2.6 | 3.5 | - |
benzylpenicillin | TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes | |
3.5.2.6 | 3.6 | - |
aztreonam | TEM-149 mutant T182M, pH 7.0, 25°C | Klebsiella aerogenes | |
3.5.2.6 | 4.7 | - |
aztreonam | TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes | |
3.5.2.6 | 5 | - |
piperacillin | TEM-149 mutant T182M, pH 7.0, 25°C | Klebsiella aerogenes | |
3.5.2.6 | 5.4 | - |
ampicillin | TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes | |
3.5.2.6 | 7.4 | - |
nitrocefin | TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes | |
3.5.2.6 | 8.3 | - |
ceftazidime | TEM-149 mutant T182M, pH 7.0, 25°C | Klebsiella aerogenes | |
3.5.2.6 | 8.3 | - |
ceftazidime | TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes | |
3.5.2.6 | 10.5 | - |
benzylpenicillin | TEM-149 mutant T182M, pH 7.0, 25°C | Klebsiella aerogenes | |
3.5.2.6 | 26 | - |
nitrocefin | TEM-149 mutant T182M, pH 7.0, 25°C | Klebsiella aerogenes |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.2.6 | 0.000018 | - |
tazobactam | TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes | |
3.5.2.6 | 0.000026 | - |
tazobactam | TEM-149T182M mutant, pH 7.0, 25°C | Klebsiella aerogenes | |
3.5.2.6 | 0.00011 | - |
clavulanic acid | TEM-149T182M mutant, pH 7.0, 25°C | Klebsiella aerogenes | |
3.5.2.6 | 0.00015 | - |
Sulbactam | TEM-149T182M mutant, pH 7.0, 25°C | Klebsiella aerogenes | |
3.5.2.6 | 0.00021 | - |
Sulbactam | TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes | |
3.5.2.6 | 0.00026 | - |
clavulanic acid | TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.5.2.6 | Klebsiella aerogenes | and above 9.0, isoelectric focusing analysis | - |
5.8 |
3.5.2.6 | Klebsiella aerogenes | above, and at 5.8, isoelectric focusing analysis | - |
9 |