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Literature summary extracted from

  • Stoczko, M.; Frere, J.; Rossolini, G.M.; Docquier, J.
    Functional diversity among metallo-beta -lactamases: characterization of the CAR-1 enzyme of Erwinia carotovora (2008), Antimicrob. Agents Chemother., 52, 2473-2479.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.6 the ECA2849 ORF is cloned and expressed in recombinant Escherichia coli strain Pectobacterium carotovorum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.11
-
 cefuroxime
-
 Pectobacterium carotovorum
3.5.2.6 0.12
-
 faropenem
-
 Pectobacterium carotovorum
3.5.2.6 0.13
-
 ceftriaxone
-
 Pectobacterium carotovorum
3.5.2.6 0.16
-
 cefotaxime
-
 Pectobacterium carotovorum
3.5.2.6 0.2
-
 penicillin G
-
 Pectobacterium carotovorum
3.5.2.6 0.2
-
 cephalothin
-
 Pectobacterium carotovorum
3.5.2.6 0.34
-
 piperacillin
-
 Pectobacterium carotovorum
3.5.2.6 0.561
-
 ceftazidime above Pectobacterium carotovorum
3.5.2.6 0.64
-
 ticarcillin
-
 Pectobacterium carotovorum
3.5.2.6 0.99
-
 meropenem above Pectobacterium carotovorum
3.5.2.6 1.57
-
 cefoxitin above Pectobacterium carotovorum
3.5.2.6 1.6
-
 ampicillin
-
 Pectobacterium carotovorum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.2.6 Zn2+
-
 Pectobacterium carotovorum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.6 35060
-
 theoretical and determined by electrospray mass spectrometry Pectobacterium carotovorum
3.5.2.6 40000
-
 gel filtration Pectobacterium carotovorum

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Pectobacterium carotovorum Q6D395 subsp. atroseptica DSM 30184 (also recently named Pectobacterium atrosepticum)
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.6 ion-exchange chromatography Pectobacterium carotovorum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 ampicillin + H2O
-
 Pectobacterium carotovorum (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 cefotaxime + H2O
-
 Pectobacterium carotovorum (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cefoxitin + H2O
-
 Pectobacterium carotovorum (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 ceftazidime + H2O
-
 Pectobacterium carotovorum (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 ceftriaxone + H2O
-
 Pectobacterium carotovorum (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[[(6-hydroxy-2-methyl-5-oxo-2,5-dihydro-1,2,4-triazin-3-yl)sulfanyl]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cefuroxime + H2O
-
 Pectobacterium carotovorum (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cephalothin + H2O
-
 Pectobacterium carotovorum (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 faropenem + H2O
-
 Pectobacterium carotovorum (2R)-2-[(1S,2R)-1-carboxy-2-hydroxypropyl]-5-[(2R)-tetrahydrofuran-2-yl]-2,3-dihydro-1,3-thiazole-4-carboxylic acid
-
 ?
3.5.2.6 meropenem + H2O
-
 Pectobacterium carotovorum (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-{[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl}-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 penicillin G + H2O
-
 Pectobacterium carotovorum (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 piperacillin + H2O
-
 Pectobacterium carotovorum (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 ticarcillin + H2O
-
 Pectobacterium carotovorum (2R,4S)-2-[(R)-carboxy[[(2R)-2-carboxy-2-(thiophen-3-yl)acetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.2.6 monomer
-
 Pectobacterium carotovorum

Synonyms

EC Number Synonyms Comment Organism
3.5.2.6 CAR-1 enzyme
-
 Pectobacterium carotovorum
3.5.2.6 MBL
-
 Pectobacterium carotovorum
3.5.2.6 metallo-beta-lactamase
-
 Pectobacterium carotovorum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 additional information
-
 additional information no hydrolysis detected with enzyme concentrations up to 4 microM for temocillin, cefepime, imipenem, and aztreonam Pectobacterium carotovorum
3.5.2.6 1
-
 meropenem above Pectobacterium carotovorum
3.5.2.6 2
-
 penicillin G
-
 Pectobacterium carotovorum
3.5.2.6 5
-
 ampicillin
-
 Pectobacterium carotovorum
3.5.2.6 5
-
 faropenem
-
 Pectobacterium carotovorum
3.5.2.6 7
-
 cefoxitin above Pectobacterium carotovorum
3.5.2.6 9
-
 piperacillin
-
 Pectobacterium carotovorum
3.5.2.6 9
-
 ticarcillin
-
 Pectobacterium carotovorum
3.5.2.6 13
-
 ceftriaxone
-
 Pectobacterium carotovorum
3.5.2.6 160
-
 ceftazidime above Pectobacterium carotovorum
3.5.2.6 240
-
 cephalothin
-
 Pectobacterium carotovorum
3.5.2.6 250
-
 cefotaxime
-
 Pectobacterium carotovorum
3.5.2.6 400
-
 cefuroxime
-
 Pectobacterium carotovorum