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Literature summary extracted from

  • Suttie, J.W.
    Vitamin K-dependent carboxylase (1985), Annu. Rev. Biochem., 54, 459-477.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.90 decarboxylated plasma prothrombin activates the enzyme Rattus norvegicus
4.1.1.90 endogenous precursor activates the enzyme Rattus norvegicus
4.1.1.90 additional information enzyme activity increases 2-3fold by a vitamin K deficiency or Warfarin treatment Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.90 2,3,5,6-Tetrachloro-4-pyridinol TCP, anticoagulant action, effective in vitro inhibitor of the carboxylase Rattus norvegicus
4.1.1.90 2-chloro-3-phytyl-1,4-naphthoquinone chloro-K, effective in vivo antagonist of vitamin K, inhibits the enzyme in an competitive fashion Rattus norvegicus
4.1.1.90 Boc-Ser(OPO4)-Ser(OPO4)-Leu-OMe inhibits the enzyme apparently competitively with regard to other peptide substrate Rattus norvegicus
4.1.1.90 CN- enzyme is blocked by mM concentrations of CN- Rattus norvegicus
4.1.1.90 Cu2+ free Cu2+ and Cu2+-complexes inhibit the reaction Rattus norvegicus
4.1.1.90 ethanol high concentrations Rattus norvegicus
4.1.1.90 Hematin
-
Rattus norvegicus
4.1.1.90 methemoglobin
-
Rattus norvegicus
4.1.1.90 p-hydroxymercuribenzoate
-
Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.1.90 microsome
-
Rattus norvegicus
-
-
4.1.1.90 rough endoplasmic reticulum
-
Rattus norvegicus 5791
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.90 additional information Mn2+, high concentrations significantly decrease or have no effect on Km of a peptide substrate for the enzyme Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.90 additional information Rattus norvegicus cis-isomer of vitamin K1, the 2-desmethyl derivative of phylloquinone, MK-1, or menadione (2 -methyl-1,4-naphthoquinone) have little or no activity ?
-
?
4.1.1.90 peptidyl-L-glutamate + CO2 + O2 + menaquinone Rattus norvegicus
-
?
-
?
4.1.1.90 peptidyl-L-glutamate + CO2 + O2 + phylloquinone Rattus norvegicus
-
peptidyl-4-carboxy L-glutamate + 2,3-epoxyphylloquinone + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.90 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.90 difficult, different methods shown Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
4.1.1.90 peptidyl-4-carboxyglutamate + 2,3-epoxyphylloquinone + H2O = peptidyl-glutamate + CO2 + O2 + phylloquinol in the physiological process reaction runs in reversed direction Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.1.90 bone
-
Rattus norvegicus
-
4.1.1.90 cartilage
-
Rattus norvegicus
-
4.1.1.90 fibroblast
-
Rattus norvegicus
-
4.1.1.90 kidney
-
Rattus norvegicus
-
4.1.1.90 kidney cell line
-
Rattus norvegicus
-
4.1.1.90 liver
-
Rattus norvegicus
-
4.1.1.90 lung
-
Rattus norvegicus
-
4.1.1.90 pancreas
-
Rattus norvegicus
-
4.1.1.90 placenta
-
Rattus norvegicus
-
4.1.1.90 spleen
-
Rattus norvegicus
-
4.1.1.90 tendon
-
Rattus norvegicus
-
4.1.1.90 testis
-
Rattus norvegicus
-
4.1.1.90 thymus
-
Rattus norvegicus
-
4.1.1.90 thyroid
-
Rattus norvegicus
-
4.1.1.90 uterus
-
Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.90 additional information cis-isomer of vitamin K1, the 2-desmethyl derivative of phylloquinone, MK-1, or menadione (2 -methyl-1,4-naphthoquinone) have little or no activity Rattus norvegicus ?
-
?
4.1.1.90 peptidyl-L-glutamate + CO2 + O2 + menaquinone
-
Rattus norvegicus ?
-
?
4.1.1.90 peptidyl-L-glutamate + CO2 + O2 + phylloquinone
-
Rattus norvegicus peptidyl-4-carboxy L-glutamate + 2,3-epoxyphylloquinone + H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.90 peptidyl-glutamate 4-carboxylase
-
Rattus norvegicus
4.1.1.90 peptidyl-glutamate 4-carboxylase (2-methyl-3-phytyl-1,4-naphthoquinone-epoxidizing)
-
Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.90 37
-
enzyme is not very stable at 37°C and lower temperatures are more desirable. At temperatures below 20°C, extended linear rates of incorporation of 14CO2 into exogenous peptide substrates can be observed Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.90 7.2 7.4 assay at. The activity falls off sharply above pH 8 or below pH 7 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.90 vitamin K
-
Rattus norvegicus