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Literature summary extracted from

  • Sigmond, J.; Honeywell, R.J.; Postma, T.J.; Dirven, C.M.; de Lange, S.M.; van der Born, K.; Laan, A.C.; Baayen, J.C.; Van Groeningen, C.J.; Bergman, A.M.; Giaccone, G.; Peters, G.J.
    Gemcitabine uptake in glioblastoma multiforme: potential as a radiosensitizer (2009), Ann. Oncol., 20, 182-187.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.4.5 medicine studies on radiosensitization of glioblastoma multiforme (GBM), deoxynucleoside analogue gemcitabine (dFdC) considered for combination therapy with radiation in glioblastomas Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.5 0.0092
-
 2',2'-difluorodeoxycytidine i.e. gemcitabine, variation of plasma gemcitabine between 0.9 and 9.2 microM Homo sapiens
3.5.4.5 0.0726
-
 2',2'-difluorodeoxyuridine i.e. dFdU, variation of plasma ranges between 24.9 and 72.6 microM Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.5 deoxycytidine + H2O Homo sapiens
-
 deoxyuridine + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.5 Homo sapiens
-
 glioblastoma multiforme (GBM) patients
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.4.5 blood plasma glioblastoma multiforme (GBM) patients Homo sapiens
-
3.5.4.5 glioblastoma multiforme cell glioblastoma multiforme (GBM) patients Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.5 additional information
-
 variation between 1.51 to 5.50 nmol/h/mg protein, about 100-fold lower than that in liver Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.5 2',2'-difluorodeoxycytidine + H2O deamination of the deoxynucleoside analogue gemcitabine (dFdC) to difluorodeoxyuridine (dFdU), difluorodeoxyuridine used as radiosensitizer in gene therapy Homo sapiens 2',2'-difluorodeoxyuridine + NH3
-
 ?
3.5.4.5 deoxycytidine + H2O
-
 Homo sapiens deoxyuridine + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.4.5 dCDA
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.4.5 37
-
 assay at Homo sapiens