Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Maguire, C.; Deliolanis, N.; Pike, L.; Niers, J.; Tjon-Kon-Fat, L.; Sena-Esteves, M.; Tannous, B.
    Gaussia luciferase variant for high-throughput functional screening applications (2009), Anal. Chem., 81, 7102-7106.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.12.5 coelenterazine increase of wild-type Gluc activity at higher substrate concentrations Gaussia princeps
1.13.12.5 Triton X-100 0.1%, 3.7fold increase of specific activity of wild-tpye Gluc Gaussia princeps

Application

EC Number Application Comment Organism
1.13.12.5 molecular biology sensitive reproter for studies of gene expression, promoter activity, protein-protein interactions, signal transduction, tumor cell growth, response to therapy Gaussia princeps

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.12.5 expression in Escherichia coli DH10B cells and 293T human kidney fibroblasts Gaussia princeps

Protein Variants

EC Number Protein Variants Comment Organism
1.13.12.5 M43I after 120 s of incubation time still 87% residual light emission compared to only 30% of wild-type Gluc, stabilized light emission with detergent Triton X-100, more stable light emission compared to wild-type Gluc when expression in mammalian cells Gaussia princeps
1.13.12.5 additional information without the signal sequence higher enzyme yield and activity Gaussia princeps

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.13.12.5 additional information secreted Gaussia princeps
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.12.5 20000
-
 SDS-PAGE Gaussia princeps

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.5 Gaussia princeps
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.12.5
-
 Gaussia princeps

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.12.5 additional information
-
 value about 100000000000 relative light units/mg protein, wild-type Gluc, in presence of Triton X-100 Gaussia princeps
1.13.12.5 additional information
-
 value about 20000000000 relative light units/mg protein, mutant M43I Gaussia princeps
1.13.12.5 additional information
-
 value about 20000000000 relative light units/mg protein, wild-type Gluc Gaussia princeps
1.13.12.5 additional information
-
 value about 30000000000 relative light units/mg protein, mutant M43I, in presence of Triton X-100 Gaussia princeps

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.5 coelenterazine + O2 20 microM, assay at pH 8.0 Gaussia princeps ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.12.5 Gaussia luciferase
-
 Gaussia princeps

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.12.5 8
-
 assay at Gaussia princeps