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Literature summary extracted from
- An enzyme-coupled continuous spectrophotometric assay for magnesium protoporphyrin IX methyltransferases (2009), Anal. Biochem., 394, 223-228.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 2.1.1.11 | analysis | a continuous enzyme-coupled spectrophotometric assay for ChlM from Synechocystis sp. PCC 6803 is reported | Synechocystis sp. PCC 6803 |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.1.1.11 | into the vector pET9a for expression in Escherichia coli BL21DE3 cells | Synechocystis sp. PCC 6803 |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.1.1.11 | Magnesium | - |
Synechocystis sp. PCC 6803 |  |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.1.1.11 | S-adenosyl-L-methionine + magnesium protoporphyrin IX | Synechocystis sp. PCC 6803 | - |
S-adenosyl-L-homocysteine + magnesium protoporphyrin IX 13-methyl ester | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.1.1.11 | Synechocystis sp. PCC 6803 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.1.1.11 | using a chelating Sepharose Fast Flow resin charged with 50 mM NiSO4 | Synechocystis sp. PCC 6803 |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.1.1.11 | S-adenosyl-L-methionine + magnesium deuteroporphyrin IX | activity assay | Synechocystis sp. PCC 6803 | S-adenosyl-L-homocysteine + magnesium deuteroporphyrin IX 13-methyl ester | - |
? | |
| 2.1.1.11 | S-adenosyl-L-methionine + magnesium protoporphyrin IX | - |
Synechocystis sp. PCC 6803 | S-adenosyl-L-homocysteine + magnesium protoporphyrin IX 13-methyl ester | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 2.1.1.11 | ChlM | - |
Synechocystis sp. PCC 6803 |
| 2.1.1.11 | magnesium protoporphyrin IX methyltransferase | - |
Synechocystis sp. PCC 6803 |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.1.1.11 | 30 | - |
activity assay | Synechocystis sp. PCC 6803 |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.1.1.11 | 7.4 | - |
continuous spectrophotometric assay | Synechocystis sp. PCC 6803 |
| 2.1.1.11 | 7.5 | - |
HPLC-based assay | Synechocystis sp. PCC 6803 |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.1.1.11 | S-adenosyl-L-methionine | - |
Synechocystis sp. PCC 6803 | |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 2.1.1.11 | Synechocystis sp. PCC 6803 | the H subunit of Mg chelatase, ChlH, stimulates ChlM activity | up |
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Release 2023.1 (January 2023)
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