BRENDA - Enzyme Database

A colorimetric assay for sulfiredoxin activity using inorganic phosphate measurement

Kim, H.; Kim, H.; Hong, S.; Rhee, S.G.; Jeong, W.; Anal. Biochem. 393, 36-40 (2009)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
1.8.98.2
drug development
Srx may be a potential target for prevention or treatment of cancer, the colorimetric assay would be useful for high-throughput screening of Srx inhibitors as demonstrated
Homo sapiens
Cloned(Commentary)
EC Number
Commentary
Organism
1.8.98.2
into the vector pET14b for expression in Escherichia coli BL21 cells
Homo sapiens
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.8.98.2
C99S
mutant, substitution of serine for Cys99 blocks the release of phosphate
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.98.2
additional information
screening for Srx inhibitors is carried out in an automated setup using 25000 chemicals
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.8.98.2
Mg2+
-
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + R-SH
Homo sapiens
-
peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + R-S-S-R
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.8.98.2
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.8.98.2
His-Srx proteins are purified from cell lysates by chromatography on a column on Ni-chelating Sepharose
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + R-SH
-
695553
Homo sapiens
peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + R-S-S-R
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.8.98.2
23
-
malachite green assay at room temperature
Homo sapiens
1.8.98.2
30
-
kinetic assay
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.98.2
7.5
-
-
Homo sapiens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.8.98.2
ATP
-
Homo sapiens
1.8.98.2
glutathione
-
Homo sapiens
1.8.98.2
thioredoxin
-
Homo sapiens
Application (protein specific)
EC Number
Application
Commentary
Organism
1.8.98.2
drug development
Srx may be a potential target for prevention or treatment of cancer, the colorimetric assay would be useful for high-throughput screening of Srx inhibitors as demonstrated
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.98.2
into the vector pET14b for expression in Escherichia coli BL21 cells
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.8.98.2
ATP
-
Homo sapiens
1.8.98.2
glutathione
-
Homo sapiens
1.8.98.2
thioredoxin
-
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.8.98.2
C99S
mutant, substitution of serine for Cys99 blocks the release of phosphate
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.98.2
additional information
screening for Srx inhibitors is carried out in an automated setup using 25000 chemicals
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.8.98.2
Mg2+
-
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + R-SH
Homo sapiens
-
peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + R-S-S-R
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.98.2
His-Srx proteins are purified from cell lysates by chromatography on a column on Ni-chelating Sepharose
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + R-SH
-
695553
Homo sapiens
peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + R-S-S-R
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.8.98.2
23
-
malachite green assay at room temperature
Homo sapiens
1.8.98.2
30
-
kinetic assay
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.98.2
7.5
-
-
Homo sapiens