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Literature summary extracted from

  • Ohtsuka, K.; Maekawa, I.; Waki, M.; Takenaka, S.
    Electrochemical assay of plasmin activity and its kinetic analysis (2009), Anal. Biochem., 385, 293-299.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.21.7 analysis electrochemical assay of plasmin activity based on a ferrocenyl peptide substrate having a plasmin-specific substrate sequence, Lys-thr-Phe-Lys, and immobilized on a gold electrode. Detection limit for plasmin is around 50 ng/ml or 0.15 mU/ml. Ratio of kcat/Km values is 0.063 microM/s Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.7 4-(2-aminoethyl)benzenesulfonyl fluoride
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.7 additional information
-
 additional information ratio of kcat/Km values is 0.063 microM/s, pH 8.0, 37°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.7 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.7 commercial preparation
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.7 Boc-Glu-Lys-Lys-4-methylcoumaryl-7-amide + H2O
-
 Homo sapiens Boc-Glu-Lys-Lys + 7-amino-4-methylcoumarin
-
 ?