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Literature summary extracted from

  • Diaz-Mochon, J.J.; Planonth, S.; Bradley, M.
    From 10,000 to 1: Selective synthesis and enzymatic evaluation of fluorescence resonance energy transfer peptides as specific substrates for chymopapain (2009), Anal. Biochem., 384, 101-105.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.22.2 medicine enzyme is used for a number of biomedical applications Carica papaya
3.4.22.6 medicine enzyme is used for a number of biomedical applications Carica papaya
3.4.22.6 medicine used for pain relief in treatment of herniated invertebral disc Carica papaya

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.2 0.027
-
 methyl red-Abu-Ser-Ala-Pro-Val-Lys-Ala-Lys(N6-(5-carboxyfluorescein))-NH2 pH 6.2 Carica papaya
3.4.22.2 0.027
-
 methyl red-Abu-Ser-Ala-Pro-Val-Lys-Ala-Lys(N6-(5-carboxyfluorescein))-NH2 at pH 6.2, in 10 mM sodium acetate, 0.1 mM EDTA and 1 mM cysteine Carica papaya
3.4.22.2 0.1015
-
 methyl red-Abu-Ser-Ala-Pro-Val-Lys-Ala-Lys(N6-(5-carboxyfluorescein))-NH2 pH 7.4 Carica papaya
3.4.22.2 0.1015
-
 methyl red-Abu-Ser-Ala-Pro-Val-Lys-Ala-Lys(N6-(5-carboxyfluorescein))-NH2 at pH 7.4, in 100 mM phosphate-buffered saline Carica papaya
3.4.22.2 0.119
-
 methyl red-Abu-Ala-Pro-Val-Lys-Lys(N5-(5-carboxyfluorescein))-NH2 pH 6.2 Carica papaya
3.4.22.2 0.119
-
 methyl red-Abu-Ala-Pro-Val-Lys-Lys(N5-(5-carboxyfluorescein))-NH2 at pH 6.2, in 10 mM sodium acetate, 0.1 mM EDTA and 1 mM cysteine Carica papaya
3.4.22.2 0.2
-
 methyl red-Abu-Ala-Pro-Val-Lys-Lys(N5-(5-carboxyfluorescein))-NH2 pH 7.4 Carica papaya
3.4.22.2 0.2
-
 methyl red-Abu-Ala-Pro-Val-Lys-Lys(N5-(5-carboxyfluorescein))-NH2 at pH 7.4, in 100 mM phosphate-buffered saline Carica papaya
3.4.22.6 0.023
-
 methyl red-Abu-Ser-Ala-Pro-Val-Lys-Ala-Lys(N6-(5-carboxyfluorescein))-NH2 pH 6.2 Carica papaya
3.4.22.6 0.023
-
 methyl red-Abu-Ser-Ala-Pro-Val-Lys-Ala-Lys(N6-(5-carboxyfluorescein))-NH2 at pH 6.2, in 10 mM sodium acetate, 0.1 mM EDTA and 1 mM cysteine Carica papaya
3.4.22.6 0.026
-
 methyl red-Abu-Ser-Ala-Pro-Val-Lys-Ala-Lys(N6-(5-carboxyfluorescein))-NH2 pH 7.4 Carica papaya
3.4.22.6 0.026
-
 methyl red-Abu-Ser-Ala-Pro-Val-Lys-Ala-Lys(N6-(5-carboxyfluorescein))-NH2 at pH 7.4, in 100 mM phosphate-buffered saline Carica papaya
3.4.22.6 0.0502
-
 methyl red-Abu-Ala-Pro-Val-Lys-Lys(N5-(5-carboxyfluorescein))-NH2 pH 7.4 Carica papaya
3.4.22.6 0.0502
-
 methyl red-Abu-Ala-Pro-Val-Lys-Lys(N5-(5-carboxyfluorescein))-NH2 at pH 7.4, in 100 mM phosphate-buffered saline Carica papaya
3.4.22.6 0.0506
-
 methyl red-Abu-Ala-Pro-Val-Lys-Lys(N5-(5-carboxyfluorescein))-NH2 pH 6.2 Carica papaya
3.4.22.6 0.0506
-
 methyl red-Abu-Ala-Pro-Val-Lys-Lys(N5-(5-carboxyfluorescein))-NH2 at pH 6.2, in 10 mM sodium acetate, 0.1 mM EDTA and 1 mM cysteine Carica papaya

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.6 additional information Carica papaya mass spectroscopy studies show unequivocally the specificity of chymopapain toward Ala, Pro, Val and Lys for positions P4 to P1 while not presenting high specificity for residues in position P1 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.2 Carica papaya
-
-
-
3.4.22.6 Carica papaya
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.6
-
 Carica papaya

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.2 commercial preparation
-
 Carica papaya
-
3.4.22.6 commercial preparation
-
 Carica papaya
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.2 methyl red-Abu-Ala-Pro-Val-Lys-Lys(N5-(5-carboxyfluorescein))-NH2 + H2O pH 6.2 or pH 7.4, 10 min, 37°C Carica papaya ?
-
 ?
3.4.22.2 methyl red-Abu-Ala-Pro-Val-Lys-Lys(N5-(5-carboxyfluorescein))-NH2 + H2O FRET 2, fluorescence resonance energy transfer peptide 2 Carica papaya methyl red-Abu-Ala-Pro-Val-Lys + Lys(N5-(5-carboxyfluorescein))-NH2
-
 ?
3.4.22.2 methyl red-Abu-Ser-Ala-Pro-Val-Lys-Ala-Lys(N5-(5-carboxyfluorescein))-NH2 + H2O pH 6.2 or pH 7.4, 10 min, 37°C Carica papaya ?
-
 ?
3.4.22.2 methyl red-Abu-Ser-Ala-Pro-Val-Lys-Ala-Lys(N6-(5-carboxyfluorescein))-NH2 + H2O FRET 1, fluorescence resonance energy transfer peptide 1 Carica papaya methyl red-Abu-Ser-Ala-Pro-Val-Lys + Ala-Lys(N6-(5-carboxyfluorescein))-NH2
-
 ?
3.4.22.6 methyl red-Abu-Ala-Pro-Val-Lys-Lys(N5-(5-carboxyfluorescein))-NH2 + H2O FRET 2, fluorescence resonance energy transfer peptide 2 Carica papaya methyl red-Abu-Ala-Pro-Val-Lys + Lys(N5-(5-carboxyfluorescein))-NH2
-
 ?
3.4.22.6 methyl red-Abu-Ala-Pro-Val-Lys-Lys(N5-(5-carboxyfluorescein))-NH2 + H2O pH 6.2 or pH 7.4, 10 min, 37°C Carica papaya methyl red-Abu-Ala-Pro-Val-Lys + Lys(N5-(5-carboxyfluorescein))-NH2
-
 ?
3.4.22.6 methyl red-Abu-Ser-Ala-Pro-Val-Lys-Ala-Lys(N5-(5-carboxyfluorescein))-NH2 + H2O pH 6.2 or pH 7.4, 10 min, 37°C Carica papaya methyl red-Abu-Ser-Ala-Pro-Val-Lys + Ala-Lys(N5-(5-carboxyfluorescein))-NH2
-
 ?
3.4.22.6 methyl red-Abu-Ser-Ala-Pro-Val-Lys-Ala-Lys(N6-(5-carboxyfluorescein))-NH2 + H2O FRET 1, fluorescence resonance energy transfer peptide 1 Carica papaya methyl red-Abu-Ser-Ala-Pro-Val-Lys + Ala-Lys(N6-(5-carboxyfluorescein))-NH2
-
 ?
3.4.22.6 additional information mass spectroscopy studies show unequivocally the specificity of chymopapain toward Ala, Pro, Val and Lys for positions P4 to P1 while not presenting high specificity for residues in position P1 Carica papaya ?
-
 ?
3.4.22.6 N-alpha-benzoyl-DL-arginine-para-nitroanilide + H2O
-
 Carica papaya ?
-
 ?
3.4.22.6 N-alpha-benzoyl-L-arginine ethyl ester + H2O
-
 Carica papaya ?
-
 ?
3.4.22.6 N-alpha-benzoylphenylalanine-arginine-4-methylcoumarin-7-amide + H2O
-
 Carica papaya ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.2 papain
-
 Carica papaya
3.4.22.6 Chymopapain
-
 Carica papaya

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.2 37
-
 assay at Carica papaya
3.4.22.2 37
-
 pH 6.2 or pH 7.4, 10 min Carica papaya
3.4.22.6 37
-
 assay at Carica papaya
3.4.22.6 37
-
 pH 6.2 or pH 7.4, 10 min Carica papaya

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.2 243
-
 methyl red-Abu-Ser-Ala-Pro-Val-Lys-Ala-Lys(N6-(5-carboxyfluorescein))-NH2 at pH 6.2, in 10 mM sodium acetate, 0.1 mM EDTA and 1 mM cysteine Carica papaya
3.4.22.2 475
-
 methyl red-Abu-Ala-Pro-Val-Lys-Lys(N5-(5-carboxyfluorescein))-NH2 at pH 6.2, in 10 mM sodium acetate, 0.1 mM EDTA and 1 mM cysteine Carica papaya
3.4.22.2 1503
-
 methyl red-Abu-Ser-Ala-Pro-Val-Lys-Ala-Lys(N6-(5-carboxyfluorescein))-NH2 at pH 7.4, in 100 mM phosphate-buffered saline Carica papaya
3.4.22.2 1773
-
 methyl red-Abu-Ala-Pro-Val-Lys-Lys(N5-(5-carboxyfluorescein))-NH2 at pH 7.4, in 100 mM phosphate-buffered saline Carica papaya
3.4.22.6 133
-
 methyl red-Abu-Ser-Ala-Pro-Val-Lys-Ala-Lys(N6-(5-carboxyfluorescein))-NH2 at pH 6.2, in 10 mM sodium acetate, 0.1 mM EDTA and 1 mM cysteine Carica papaya
3.4.22.6 246
-
 methyl red-Abu-Ala-Pro-Val-Lys-Lys(N5-(5-carboxyfluorescein))-NH2 at pH 6.2, in 10 mM sodium acetate, 0.1 mM EDTA and 1 mM cysteine Carica papaya
3.4.22.6 360
-
 methyl red-Abu-Ser-Ala-Pro-Val-Lys-Ala-Lys(N6-(5-carboxyfluorescein))-NH2 at pH 7.4, in 100 mM phosphate-buffered saline Carica papaya
3.4.22.6 590
-
 methyl red-Abu-Ala-Pro-Val-Lys-Lys(N5-(5-carboxyfluorescein))-NH2 at pH 7.4, in 100 mM phosphate-buffered saline Carica papaya

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.6 6.2
-
 assay at Carica papaya
3.4.22.6 7.4
-
 assay at Carica papaya

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.2 6.2 7.4 10 min, 37°C Carica papaya
3.4.22.6 additional information
-
 chymopapain has a broad working pH range Carica papaya
3.4.22.6 6.2 7.4 10 min, 37°C Carica papaya