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Literature summary extracted from

  • Zhang, L.S.; Mummert, M.E.
    Development of a fluorescent substrate to measure hyaluronidase activity (2008), Anal. Biochem., 379, 80-85.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.2.1.35 biotechnology a fluorescent substrate (FRET-HA) to quantitatively assess hyaluronidase activity is developed Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.35 Bos taurus
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.35 testis
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 Bos taurus
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.35 hyaluronan + H2O a fluorescent substrate (FRET-HA) to quantitatively assess hyaluronidase activity is developed. Hyaluronan (HA)is dual labeled with fluorescein amine and rhodamine B amine. The fluorescein amine fluorescence signal is significantly quenched and the rhodamine B amine signal is significantly enhanced due to fluorescence resonance energy transfer (FRET). In the presence of bovine testes hyaluronidase, cleavage of HA disrupts FRET, resulting in a loss of the fluorescein amine quenching that is dependent on both enzyme concentration and time. Increase in the fluorescein amine signal can be conveniently monitored in both noncontinuous and continuous fashions Bos taurus ?
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Synonyms

EC Number Synonyms Comment Organism
3.2.1.35 hyaluronidase
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 Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.35 37
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 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.35 6
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 assay at Bos taurus