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Literature summary extracted from

  • Cornell, H.J.; Doherty, W.; Stelmasiak, T.
    Papaya latex enzymes capable of detoxification of gliadin (2009), Amino Acids, 38, 155-165.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.2.5 drug development QCT would be suitable for enzyme therapy in gluten intolerance and appear to have synergistic action with porcine intestinal extracts, potential for enzyme therapy in coeliac disease Carica papaya
3.4.22.30 drug development caricain would be suitable for enzyme therapy in gluten intolerance and appears to have synergistic action with porcine intestinal extracts, potential for enzyme therapy in coeliac disease Carica papaya

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.5 30000
-
 fraction are estimated from plots of Kav against molecular weight using dextran blue, human gamma-globulin, bovine serum albumin and trypsin inhibitor as standards, mass spectrometric analysis of trypsin-digested fractions from chromatography is carried out by liquid chromatography/electrospray quadrupole time-of-flight mass spectrometry Carica papaya
3.4.22.6 36000
-
 fraction is estimated from plots of Kav against molecular weight using dextran blue, human gamma-globulin, bovine serum albumin and trypsin inhibitor as standards, mass spectrometric analysis of trypsin-digested fractions from chromatography are carried out by liquid chromatography/electrospray quadrupole time-of-flight mass spectrometry in positive mode Carica papaya
3.4.22.30 40000
-
 procaricain, fractions are estimated from plots of Kav against molecular weight using dextran blue, human gamma-globulin, bovine serum albumin and trypsin inhibitor as standards, mass spectrometric analysis of trypsin-digested fractions from chromatography are carried out by liquid chromatography/electrospray quadrupole time-of-flight mass spectrometry in positive mode Carica papaya

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.30 gliadin + H2O Carica papaya
-
 fragments of gliadin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.5 Carica papaya
-
-
-
2.3.2.5 Carica papaya O81226
-
-
3.4.21.26 Carica papaya
-
 estimated as a fraction of papaya latex
-
3.4.22.2 Carica papaya
-
 estimated as a fraction of papaya latex
-
3.4.22.6 Carica papaya
-
-
-
3.4.22.6 Carica papaya P14080
-
-
3.4.22.30 Carica papaya
-
-
-
3.4.22.30 Carica papaya P10056
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.5 enzyme is semi-purified from latex crude extracts by ion exchange chromatography on a CM Sephadex column C-50 and gel filtration Carica papaya
3.4.22.6
-
 Carica papaya
3.4.22.6 enzyme is semi-purified with from latex crude extracts by ion exchange chromatography on a CM Sephadex column C-50 and gel filtration Carica papaya
3.4.22.30 enzyme is semi-purified with from latex crude extracts by ion exchange chromatography on a CM Sephadex column C-50 and gel filtration Carica papaya

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.5 latex
-
 Carica papaya
-
2.3.2.5 latex estimated as a fraction of papaya latex Carica papaya
-
3.4.21.26 latex estimated as a fraction of papaya latex Carica papaya
-
3.4.22.2 latex estimated as a fraction of papaya latex Carica papaya
-
3.4.22.6 latex
-
 Carica papaya
-
3.4.22.6 latex estimated as a fraction of papaya latex Carica papaya
-
3.4.22.30 latex
-
 Carica papaya
-
3.4.22.30 latex estimated as a fraction of papaya latex Carica papaya
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.26 Z-Gly-Pro-4-nitroanilide + H2O pH 7.0, room temperature Carica papaya 4-nitroaniline + Z-Gly-Pro
-
 ?
3.4.22.2 benzoyl arginine ethyl ester + H2O pH 6.3, 20°C Carica papaya ?
-
 ?
3.4.22.30 gliadin + H2O
-
 Carica papaya fragments of gliadin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.5 glutamine cyclotransferase
-
 Carica papaya
2.3.2.5 QCT
-
 Carica papaya
3.4.21.26 PEP
-
 Carica papaya
3.4.21.26 prolyl endopeptidase
-
 Carica papaya
3.4.22.2 papain
-
 Carica papaya
3.4.22.6 Chymopapain
-
 Carica papaya
3.4.22.30 caricain
-
 Carica papaya
3.4.22.30 Papaya proteinase OMEGA
-
 Carica papaya
3.4.22.30 procaricain
-
 Carica papaya

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.26 additional information
-
 room temperature, pH 7.0 Carica papaya
3.4.22.2 20
-
 pH 6.3 Carica papaya

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.2.5 85
-
 heat stability of QCT up to 85°C Carica papaya
3.4.22.30 85
-
 heat stability of caricain up to 85°C Carica papaya

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.5 8.5
-
 enzyme is dissolved in phosphate buffer and used with a suspension of lysosomes in 0.154 mol/L NaCl Carica papaya
3.4.21.26 7
-
 room temperature Carica papaya
3.4.22.2 6.3
-
 20°C Carica papaya
3.4.22.6 7.2
-
 enzyme is dissolved in phosphate buffer and used with a suspension of lysosomes in 0.154 mol/l sodium chloride Carica papaya
3.4.22.30 6.8
-
 enzyme is dissolved in phosphate buffer and used with a suspension of lysosomes in 0.154 mol/l sodium chloride Carica papaya