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Literature summary extracted from

  • Nakasaki, T.; Tanaka, T.; Okudaira, S.; Hirosawa, M.; Umemoto, E.; Otani, K.; Jin, S.; Bai, Z.; Hayasaka, H.; Fukui, Y.; Aozasa, K.; Fujita, N.; Tsuruo, T.; Ozono, K.; Aoki, J.; Miyasaka, M.
    Involvement of the lysophosphatidic acid-generating enzyme autotaxin in lymphocyte-endothelial cell interactions (2008), Am. J. Pathol., 173, 1566-1576.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.39 co-transfection of MBEC4 cells with 2 plasmids, mlysoPLDpCAGGS26 and pSV2-neo, screening by Western blotting and immunocytochemistry with an anti-ATX mAb (4F1)14 and by measuring the lysoPLD activities of the culture media Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.39 100000
-
 approx., Western blot, ATX expressed in MBEC4-ATX cells Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.39 lysophosphatidylcholine + H2O Mus musculus
-
 lysophosphatidic acid + choline
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.39 Mus musculus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.4.39 glycoprotein
-
 Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.39 blood plasma
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.39 additional information
-
 approx. 400 micromol/min/ml, mice are treated with control rat IgG, plasma samples (10 microl) incubate with 4 mmol/l lysophosphatidylcholine, activity determined by enzymatic photometric method using choline oxidase at 560 nm, 37°C Mus musculus
3.1.4.39 additional information
-
 approx. 42 micromol/min/ml, mice are treated with anti-ATX mAb, plasma samples (10 microl) incubate with 4 mmol/l lysophosphatidylcholine, activity determined by enzymatic photometric method using choline oxidase at 560 nm, 37°C Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.39 lysophosphatidylcholine + H2O
-
 Mus musculus lysophosphatidic acid + choline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.39 ATX
-
 Mus musculus
3.1.4.39 autotaxin
-
 Mus musculus
3.1.4.39 lysoPLD
-
 Mus musculus