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Literature summary extracted from
- Cloning, purification, crystallization and preliminary crystallographic analysis of a ribokinase from Staphylococcus aureus (2009), Acta Crystallogr. Sect. F, 65, 574-576.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.7.1.15 | the SUMO-Sa239 fusion protein is expressed in Escherichia coli BL21 (DE3) cells | Staphylococcus aureus |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 2.7.1.15 | by using the hanging-drop vapour-diffusion method, large crystals appear at 21.85°C in conditions consisting of 23% PEG 3000, 0.1 M sodium citrate pH 5.6, the crystals diffract to 2.9 A resolution and belong to space group P6122 or P6522, with unit-cell parameters a=b=91.8 A, c=160.7 A | Staphylococcus aureus |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.7.1.15 | Mg2+ | the catalytic reactivity of ribokinase is activated by the divalent cation Mg2+, in the absence of these metal ions, ribokinase loses its catalytic ability | Staphylococcus aureus |  |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.7.1.15 | ATP + D-ribose | Staphylococcus aureus | - |
ADP + D-ribose 5-phosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.7.1.15 | Staphylococcus aureus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.7.1.15 | by affinity chromatography on a nickel-chelating column and gel filtration | Staphylococcus aureus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.7.1.15 | ATP + D-ribose | - |
Staphylococcus aureus | ADP + D-ribose 5-phosphate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 2.7.1.15 | ribokinase | - |
Staphylococcus aureus |
| 2.7.1.15 | Sa239 | - |
Staphylococcus aureus |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 2.7.1.15 | physiological function | ribokinase plays a key role in the recycling of sugars produced from the metabolism of nucleotides | Staphylococcus aureus |
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