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Literature summary extracted from

  • Cameron, S.; Martini, V.P.; Iulek, J.; Hunter, W.N.
    Geobacillus stearothermophilus 6-phosphogluconate dehydrogenase complexed with 6-phosphogluconate (2009), Acta Crystallogr. Sect. F, 65, 450-454.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.44 expressed in Escherichia coli BL21 (DE3) pLysS cells Geobacillus stearothermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.44 6-phosphogluconate dehydrogenase complexed with 6-phosphogluconate, hanging drop vapour diffusion method, using 0.2 M lithium sulfate, 2.2 M ammonium sulfate or 0.2 M ammonium acetate, 2.2 M ammonium sulfate Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.44 Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.44 HisTrap column chromatography and Superdex 200 gel filtration Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.44 6-phospho-D-gluconate + NADP+
-
 Geobacillus stearothermophilus D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.44 homodimer x-ray crystallography Geobacillus stearothermophilus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.44 6-phosphogluconate dehydrogenase
-
 Geobacillus stearothermophilus
1.1.1.44 6PDH
-
 Geobacillus stearothermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.44 NADP+
-
 Geobacillus stearothermophilus