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Literature summary extracted from

  • Prudnikova, T.; Mozga, T.; Rezacova, P.; Chaloupkova, R.; Sato, Y.; Nagata, Y.; Brynda, J.; Kuty, M.; Damborsky, J.; Smatanova, I.K.
    Crystallization and preliminary X-ray analysis of a novel haloalkane dehalogenase DbeA from Bradyrhizobium elkani USDA94 (2009), Acta Crystallogr. Sect. F, 65, 353-356.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.8.1.5 expressed in Escherichia coli BL21 (DE3) cells Bradyrhizobium elkanii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.8.1.5 wild type DbeA and mutant enzyme DbeA1, which carries the unique fragment of DbjA, are crystallized using the sitting-drop vapour-diffusion method, in 100 mM Tris-HCl buffer pH 7.5, containing 25% (w/v) PEG 3350 and 130 mM calcium acetate, at 4°C and 20°C Bradyrhizobium elkanii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.8.1.5 35000
-
 SDS-PAGE Bradyrhizobium elkanii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.8.1.5 additional information Bradyrhizobium elkanii DbeA is less active and has a higher specificity towards brominated and iodinated compounds compared with DbjA ?
-
 ?
3.8.1.5 additional information Bradyrhizobium elkanii USDA94 DbeA is less active and has a higher specificity towards brominated and iodinated compounds compared with DbjA ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.5 Bradyrhizobium elkanii
-
-
-
3.8.1.5 Bradyrhizobium elkanii USDA94
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.8.1.5 Ni-NTA Sepharose column chromatography Bradyrhizobium elkanii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.5 additional information DbeA is less active and has a higher specificity towards brominated and iodinated compounds compared with DbjA Bradyrhizobium elkanii ?
-
 ?
3.8.1.5 additional information DbeA is less active and has a higher specificity towards brominated and iodinated compounds compared with DbjA Bradyrhizobium elkanii USDA94 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.8.1.5 DbeA
-
 Bradyrhizobium elkanii