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Literature summary extracted from

  • Komori, H.; Miyazaki, K.; Higuchi, Y.
    Crystallization and preliminary X-ray diffraction analysis of a putative two-domain-type laccase from a metagenome (2009), Acta Crystallogr. Sect. F, 65, 264-266.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.10.3.2 additional information the enzyme may be used in a variety of biotechnological applications, including textile dye bleaching, pulp bleaching, bioremediation, polymer synthesis and biosensors Thermus thermophilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.3.2 expression of a His-tagged two-type domain laccase, lacking the signal sequence domain, in Escherichia coli strain BL21(DE3) Thermus thermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.10.3.2 two-domain-type by sitting-drop vapour-diffusion method, mixing of 0.001 ml of a protein solution, containing 8.5 mg/ml protein, with 0.001 ml of reservoir solution, containing 0.2 M potassium chloride, 0.05 M HEPES, pH 7.5, and 35% v/v pentaerythritol propoxylate, at 20°C, X-ray diffraction structure determination and analysis at 1.7 A resolution, single-wavelength anomalous diffraction technique using Cu atoms Thermus thermophilus

Protein Variants

EC Number Protein Variants Comment Organism
1.10.3.2 additional information construction of a two-type domain laccase from a three-type domain laccase by eliminating the signal sequence domain Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Cu2+ a multicopper protein Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.10.3.2 additional information Thermus thermophilus the enzyme oxidizes a range of phenolic and nonphenolic compounds using molecular oxygen as an electron acceptor to produce water with a broad substrate specificity ?
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 ?

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Thermus thermophilus
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 two-domain-type laccase from a metagenome
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Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 recombinant His-tagged two-type domain laccase from Escherichia coli strain BL21(DE3) by heat treatment, centrifugation, nickel affinity chromatography, and ultrafiltration Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 additional information the enzyme oxidizes a range of phenolic and nonphenolic compounds using molecular oxygen as an electron acceptor to produce water with a broad substrate specificity Thermus thermophilus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.10.3.2 Benzenediol:oxygen oxidoreductase
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 Thermus thermophilus