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Literature summary extracted from

  • Bowyer, A.; Mikolajek, H.; Wright, J.N.; Coker, A.; Erskine, P.T.; Cooper, J.B.; Bashir, Q.; Rashid, N.; Jamil, F.; Akhtar, M.
    Crystallization and preliminary X-ray diffraction analysis of L-threonine dehydrogenase (TDH) from the hyperthermophilic archaeon Thermococcus kodakaraensis (2008), Acta Crystallogr. Sect. F, 64, 828-830.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.103 ligated into the pTZ57R/T cloning vector. The recombinant pTZ-tdh plasmid digested with NcoI and EcoRI and the TDH gene ligated with pET-8c, giving the recombinant pET-tdh plasmid, used to transform Escherichia coli DH5alpha competent cells. Recombinant and purified pET-tdh plasmid expressed in Escherichia coli BL21 (DE3) Thermococcus kodakarensis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.103 by hanging-drop vapour-diffusion method, to 2.6 A resolution. Crystals grow in the tetragonal space group P43212, with unit-cell parameters a=b=124.5, c=271.1 A. There are four molecules in the asymmetric unit of the crystal Thermococcus kodakarensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.103 38016
-
 4 * 38016, electrospray mass spectrometry and gel filtration Thermococcus kodakarensis

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.103 Thermococcus kodakarensis Q5JI69
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.103 by sonication, centrifugation, heating of the soluble fraction, anion-exchange and hydrophobic interaction chromatography Thermococcus kodakarensis

Storage Stability

EC Number Storage Stability Organism
1.1.1.103 50 mM Tris–HCl buffer, pH 8.0 Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.103 L-threonine + NAD+
-
 Thermococcus kodakarensis (2S)-2-amino-3-oxobutanoate + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.103 homotetramer 4 * 38016, electrospray mass spectrometry and gel filtration Thermococcus kodakarensis

Synonyms

EC Number Synonyms Comment Organism
1.1.1.103 L-threonine dehydrogenase
-
 Thermococcus kodakarensis
1.1.1.103 TDH
-
 Thermococcus kodakarensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.103 NAD+
-
 Thermococcus kodakarensis