Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ogata, H.; Goenka Agrawal, A.; Kaur, A.P.; Goddard, R.; Gaertner, W.; Lubitz, W.
    Purification, crystallization and preliminary X-ray analysis of adenylylsulfate reductase from Desulfovibrio vulgaris Miyazaki F (2008), Acta Crystallogr. Sect. F, 64, 1010-1012.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.8.99.2 hanging drop vapor diffusion method, using 0.1 M HEPES pH 7.5, 0.1 M NaCl, 2.0 M ammonium sulfate and 15% (v/v) glycerol, at 4°C Desulfovibrio vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.99.2 75000
-
 1 * 75000 + 1 * ?, X-ray crystallography Desulfovibrio vulgaris

Organism

EC Number Organism UniProt Comment Textmining
1.8.99.2 Desulfovibrio vulgaris B8PS61 and B8DS74 alpha subunit and beta subunit
-
1.8.99.2 Desulfovibrio vulgaris Miyazaki F B8PS61 and B8DS74 alpha subunit and beta subunit
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.99.2 DEAE-Toyopearl 650S column chromatography, Sephacryl S-200 gel filtration, and Superdex 200 gel filtration Desulfovibrio vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.99.2 adenylyl sulfate + reduced acceptor
-
 Desulfovibrio vulgaris AMP + sulfite + acceptor
-
 ?
1.8.99.2 adenylyl sulfate + reduced acceptor
-
 Desulfovibrio vulgaris Miyazaki F AMP + sulfite + acceptor
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.99.2 heterodimer 1 * 75000 + 1 * ?, X-ray crystallography Desulfovibrio vulgaris

Synonyms

EC Number Synonyms Comment Organism
1.8.99.2 adenylylsulfate reductase
-
 Desulfovibrio vulgaris
1.8.99.2 APS reductase
-
 Desulfovibrio vulgaris

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.99.2 4Fe-4S-center contains two 4Fe-4S clusters in the beta-subunit Desulfovibrio vulgaris
1.8.99.2 FAD contains a noncovalently bound FAD Desulfovibrio vulgaris