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Literature summary extracted from

  • Tsai, L.C.; Huang, H.C.; Hsiao, C.H.; Chiang, Y.N.; Shyur, L.F.; Lin, Y.S.; Lee, S.H.
    Mutational and structural studies of the active-site residues in truncated Fibrobacter succinogenes1,3-1,4-beta-D-glucanase (2008), Acta Crystallogr. Sect. D, 64, 1259-1266.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.73 plasmid carrying the truncated 1,3-1,4-beta-D-glucanase gene in the pET26b(+) vector, transformed into Escherichia coli XL1-Blue. Overexpression of the truncated form and mutants in Escherichia coli BL21 (DE3) cells Fibrobacter succinogenes

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.73 mutant E85I, at 2.2 A resolution, by hanging-drop vapour-diffusion method at room temperature. The mutant crystallizes in space group P3121 with one molecule in the asymmetric unit, Ca2+ ion-binding site is maintained Fibrobacter succinogenes

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.73 E85D has 5fold lower kcat/Km ratios than the wild-type Fibrobacter succinogenes
3.2.1.73 E85I has 5fold lower kcat/Km ratios than the wild-type Fibrobacter succinogenes
3.2.1.73 N72A has 11fold lower kcat/Km ratios than the wild-type Fibrobacter succinogenes
3.2.1.73 N72Q has 17fold lower kcat/Km ratios than the wild-type Fibrobacter succinogenes
3.2.1.73 Q70A has 299fold lower kcat/Km ratios than the wild-type Fibrobacter succinogenes
3.2.1.73 Q70D has 62fold lower kcat/Km ratios than the wild-type Fibrobacter succinogenes
3.2.1.73 Q70E has 106fold lower kcat/Km ratios than the wild-type Fibrobacter succinogenes
3.2.1.73 Q70I has 499fold lower kcat/Km ratios than the wild-type Fibrobacter succinogenes
3.2.1.73 Q70N has 63fold lower kcat/Km ratios than the wild-type Fibrobacter succinogenes
3.2.1.73 Q70R has 35fold lower kcat/Km ratios than the wild-type Fibrobacter succinogenes
3.2.1.73 Q81I has 2fold lower kcat/Km ratios than the wild-type Fibrobacter succinogenes
3.2.1.73 Q81N has 2.5fold lower kcat/Km ratios than the wild-type Fibrobacter succinogenes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.73 Ca2+
-
 Fibrobacter succinogenes
3.2.1.73 Cs2+
-
 Fibrobacter succinogenes

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.73 Fibrobacter succinogenes P17989
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.73 mutants purified on Ni-NTA affinity column, more than 95% purity Fibrobacter succinogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.73 236
-
 mutant Q70I Fibrobacter succinogenes
3.2.1.73 527
-
 mutant Q70A Fibrobacter succinogenes
3.2.1.73 802
-
 mutant Q70N Fibrobacter succinogenes
3.2.1.73 1089
-
 mutant N72Q Fibrobacter succinogenes
3.2.1.73 1381
-
 mutant Q70D Fibrobacter succinogenes
3.2.1.73 1385
-
 mutant Q70E Fibrobacter succinogenes
3.2.1.73 1699
-
 mutant E85I Fibrobacter succinogenes
3.2.1.73 1774
-
 mutant N72A Fibrobacter succinogenes
3.2.1.73 3076
-
 mutant E85D Fibrobacter succinogenes
3.2.1.73 3104
-
 mutant Q70R Fibrobacter succinogenes
3.2.1.73 5758
-
 mutant Q81N Fibrobacter succinogenes
3.2.1.73 7873
-
 mutant Q81I Fibrobacter succinogenes
3.2.1.73 8478
-
 truncated enzyme Fibrobacter succinogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.73 lichenan + H2O
-
 Fibrobacter succinogenes ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.73 1,3-1,4-beta-D-glucanase
-
 Fibrobacter succinogenes

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.73 109
-
 lichenan mutant Q70I, calculated as D-glucose units released from lichenan Fibrobacter succinogenes
3.2.1.73 244
-
 lichenan mutant Q70A, calculated as D-glucose units released from lichenan Fibrobacter succinogenes
3.2.1.73 371
-
 lichenan mutant Q70N, calculated as D-glucose units released from lichenan Fibrobacter succinogenes
3.2.1.73 504
-
 lichenan mutant N72Q, calculated as D-glucose units released from lichenan Fibrobacter succinogenes
3.2.1.73 639
-
 lichenan mutant Q70D, calculated as D-glucose units released from lichenan Fibrobacter succinogenes
3.2.1.73 641
-
 lichenan mutant Q70E, calculated as D-glucose units released from lichenan Fibrobacter succinogenes
3.2.1.73 785
-
 lichenan mutant E85I, calculated as D-glucose units released from lichenan Fibrobacter succinogenes
3.2.1.73 820
-
 lichenan mutant N72A, calculated as D-glucose units released from lichenan Fibrobacter succinogenes
3.2.1.73 1422
-
 lichenan mutant E85D, calculated as D-glucose units released from lichenan Fibrobacter succinogenes
3.2.1.73 1435
-
 lichenan mutant Q70R, calculated as D-glucose units released from lichenan Fibrobacter succinogenes
3.2.1.73 2663
-
 lichenan mutant Q81N, calculated as D-glucose units released from lichenan Fibrobacter succinogenes
3.2.1.73 3641
-
 lichenan mutant Q81I, calculated as D-glucose units released from lichenan Fibrobacter succinogenes
3.2.1.73 3920
-
 lichenan truncated enzyme, calculated as D-glucose units released from lichenan Fibrobacter succinogenes