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Literature summary extracted from

  • Yamamoto, H.; Takio, K.; Sugahara, M.; Kunishima, N.
    Structure of a haloacid dehalogenase superfamily phosphatase PH1421 from Pyrococcus horikoshii OT3: oligomeric state and thermoadaptation mechanism (2008), Acta Crystallogr. Sect. D, 64, 1068-1077.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.8.1.2 expressed in Escherichia coli BL21-Codon Plus (DE3)-RIL cells Pyrococcus horikoshii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.8.1.2 oil-microbatch method, in 200 mM NaCl and 20 mM Tris-HCl pH 8.0, 25.5% (w/v) polyethylene glycol 4000, 0.17 M ammonium acetate, 15% (w/v) glycerol and 85 mM sodium acetate buffer, pH 4.6, at 20°C Pyrococcus horikoshii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.8.1.2 Mg2+ dependent on Pyrococcus horikoshii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.8.1.2 25600
-
 SDS-PAGE Pyrococcus horikoshii
3.8.1.2 49700
-
 calculated apparent molecular weight Pyrococcus horikoshii

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.2 Pyrococcus horikoshii
-
 strain OT3
-
3.8.1.2 Pyrococcus horikoshii OT-3
-
 strain OT3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.8.1.2 HiPrep 26/10 column chromatography, Super Q Toyopearl 650M column chromatography, hydroxyapatite column chromatography, and Superdex 200 gel filtration Pyrococcus horikoshii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.2 additional information PH1421 is a magnesium-dependent haloacid dehalogenase phosphatase that acts on compounds closely related to 2-phosphoglycolate, the enzyme does possess 4-nitrophenyl phosphate hydrolase activity in the presence of Mg2+ Pyrococcus horikoshii ?
-
 ?
3.8.1.2 additional information PH1421 is a magnesium-dependent haloacid dehalogenase phosphatase that acts on compounds closely related to 2-phosphoglycolate, the enzyme does possess 4-nitrophenyl phosphate hydrolase activity in the presence of Mg2+ Pyrococcus horikoshii OT-3 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.8.1.2 homodimer 2 * 25600, SDS-PAGE Pyrococcus horikoshii

Synonyms

EC Number Synonyms Comment Organism
3.8.1.2 L-2-haloacid dehalogenase
-
 Pyrococcus horikoshii
3.8.1.2 PH1421
-
 Pyrococcus horikoshii