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Literature summary extracted from

  • Jeyakanthan, J.; Rangarajan, S.; Mridula, P.; Kanaujia, S.P.; Shiro, Y.; Kuramitsu, S.; Yokoyama, S.; Sekar, K.
    Observation of a calcium-binding site in the gamma-class carbonic anhydrase from Pyrococcus horikoshii (2008), Acta Crystallogr. Sect. D, 64, 1012-1019.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.1 expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells Pyrococcus horikoshii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.1 in complex with Ca2+, sitting drop vapour diffusion method, with 22.5% PEG 4000 in MES-NaOH pH 5.8 and 0.1 M lithium chloride as the precipitating agent Pyrococcus horikoshii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.1 Ca2+ the native enzyme contains four Ca2+ ions Pyrococcus horikoshii
4.2.1.1 Zn2+ zinc-containing metalloenzyme, the asymmetric unit contains a catalytic Zn2+ ion Pyrococcus horikoshii

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.1 Pyrococcus horikoshii O59257 strain OT3
-
4.2.1.1 Pyrococcus horikoshii OT-3 O59257 strain OT3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.1 Super Q Toyopearl 650M column chromatography, Resource Q column chromatography, Bio-Scale CHT-20-I column chromatography, and Superdex 200pg gel filtration Pyrococcus horikoshii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.1 CO2 + H2O
-
 Pyrococcus horikoshii H2CO3
-
 r
4.2.1.1 CO2 + H2O
-
 Pyrococcus horikoshii OT-3 H2CO3
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.2.1.1 carbonic anhydrase
-
 Pyrococcus horikoshii