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Literature summary extracted from

  • Wladyka, B.; Bista, M.; Sabat, A.J.; Bonar, E.; Grzeszczuk, S.; Hryniewicz, W.; Dubin, A.
    A novel member of the thermolysin family, cloning and biochemical characterization of metalloprotease from Staphylococcus pseudintermedius (2008), Acta Biochim. Pol., 55, 525-536.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.24.27 Calcium supplementation of growth medium by calcium induces expression. Regulation by calcium ions is at posttranscriptional level Staphylococcus pseudintermedius

General Stability

EC Number General Stability Organism
3.4.24.27 enzyme is stable in presence of CaCl2 Staphylococcus pseudintermedius

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.27 1,10-phenanthroline 5 mM, complete inhibition Staphylococcus pseudintermedius
3.4.24.27 alpha2-Macroglobulin
-
 Staphylococcus pseudintermedius
3.4.24.27 EDTA 5 mM, complete inhibition Staphylococcus pseudintermedius
3.4.24.27 additional information not inhibitory: human blood serpins alpha-1-antitrypsin and alpha-1-antichymotrypsin Staphylococcus pseudintermedius

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.27 extracellular supplementation of growth medium by calcium induces expression. Regulation by calcium ions is at posttranscriptional level Staphylococcus pseudintermedius
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.27 35000
-
 x * 35000, SDS-PAGE, x * 55170, calculated Staphylococcus pseudintermedius
3.4.24.27 55170
-
 x * 35000, SDS-PAGE, x * 55170, calculated Staphylococcus pseudintermedius

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.27 Staphylococcus pseudintermedius
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.27 incubation of impure enzyme at 37°C causes proteolysis of impurities, whereas the protease remains active and uncleaved Staphylococcus pseudintermedius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.27 alpha-1-antichymotrypsin + H2O cleavage within the sequence LSA-LVE Staphylococcus pseudintermedius ?
-
 ?
3.4.24.27 alpha-1-antitrypsin + H2O cleavage within the sequence AMF-LEA Staphylococcus pseudintermedius ?
-
 ?
3.4.24.27 azocasein + H2O
-
 Staphylococcus pseudintermedius ?
-
 ?
3.4.24.27 Azocoll + H2O
-
 Staphylococcus pseudintermedius ?
-
 ?
3.4.24.27 hide powder azure + H2O
-
 Staphylococcus pseudintermedius ?
-
 ?
3.4.24.27 additional information preferentially, peptide bonds preceding hydrophobic residues are hydrolyzed, such as I, L or F at P1' position. The enzyme less frequently also cleaves substrates with hydrophilic residues such as E, T or Q in the P1' position Staphylococcus pseudintermedius ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.27 ? x * 35000, SDS-PAGE, x * 55170, calculated Staphylococcus pseudintermedius

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.24.27 37
-
 incubation of impure enzyme at 37°C causes proteolysis of impurities, whereas the protease remains active and uncleaved Staphylococcus pseudintermedius