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Literature summary extracted from

  • Yamasaki, Y.; Nakashima, S.; Konno, H.
    Pullulanase from rice endosperm (2008), Acta Biochim. Pol., 55, 507-510.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.41 beta-cyclodextrin
-
 Oryza sativa
3.2.1.41 Co2+ 5 mM, at least 60% inhibition Oryza sativa
3.2.1.41 Cu2+ 5 mM, at least 60% inhibition Oryza sativa
3.2.1.41 Hg2+ 5 mM, at least 60% inhibition Oryza sativa
3.2.1.41 pullulan substrate inhibition above 0.1% Oryza sativa
3.2.1.41 Zn2+ 5 mM, at least 60% inhibition Oryza sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.41 100000
-
 1 * 100000, SDS-PAGE Oryza sativa
3.2.1.41 105000
-
 gel filtration Oryza sativa

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.41 Oryza sativa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.41
-
 Oryza sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.41 seed pullulanase from germinating and nongerminating seed are the same protein Oryza sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.41 amylopectin + H2O
-
 Oryza sativa maltooligosaccharides + maltose 34.1% of the rate with pullulan ?
3.2.1.41 beta-limit dextrin + H2O
-
 Oryza sativa ? 19.5% of the rate with pullulan ?
3.2.1.41 glycogen + H2O
-
 Oryza sativa maltooligosaccharides + maltose 6.3% of the rate with pullulan ?
3.2.1.41 additional information enzyme exhibits alpha-glucosyltransfer activity and produces an alpha-(1-6) linked compound of two maltotriose molecules from pullulan Oryza sativa ?
-
 ?
3.2.1.41 pullulan + H2O preferred substrate Oryza sativa maltotriose + ?
-
 ?
3.2.1.41 soluble starch
-
 Oryza sativa maltotriose + maltose 15.8% of the rate with pullulan ?

Subunits

EC Number Subunits Comment Organism
3.2.1.41 monomer 1 * 100000, SDS-PAGE Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.41 55
-
-
 Oryza sativa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.41 50
-
 stable up to Oryza sativa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.41 2.18
-
 amylopectin 37°C, pH 6.0 Oryza sativa
3.2.1.41 2.89
-
 soluble starch 37°C, pH 6.0 Oryza sativa
3.2.1.41 3.31
-
 beta-limit dextrin 37°C, pH 6.0 Oryza sativa
3.2.1.41 23.1
-
 pullulan 37°C, pH 6.0 Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.41 6
-
-
 Oryza sativa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.41 5.5 8 at 30°C Oryza sativa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.41 Oryza sativa
-
-
 4.7