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Literature summary extracted from
- Identification and cloning of a gene encoding tannase (tannin acylhydrolase) from Lactobacillus plantarum ATCC 14917(T) (2008), Syst. Appl. Microbiol., 31, 269-277.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.1.20 | expressed in Escherichia coli DH5alpha | Lactiplantibacillus plantarum |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.20 | 0.62 | - |
methyl gallate | at pH 8.0 | Lactiplantibacillus plantarum |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.20 | 50000 | - |
SDS-PAGE | Lactiplantibacillus plantarum |
| 3.1.1.20 | 50700 | - |
deduced from the amino acid sequence | Lactiplantibacillus plantarum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.20 | Lactiplantibacillus plantarum | B3Y018 | strain ATCC 14917(T) | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.20 | ammonium sulfate precipitation, Q-Sepharose column chromatography, hydroxylapatite column chromatography, and Mono-Q column chromatography | Lactiplantibacillus plantarum |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.20 | 7.5 | - |
crude extract, at pH 8.0 | Lactiplantibacillus plantarum |
| 3.1.1.20 | 84.34 | - |
after 11.25fold purification, at pH 8.0 | Lactiplantibacillus plantarum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.20 | methyl gallate + H2O | - |
Lactiplantibacillus plantarum | gallic acid + methanol | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.20 | monomer | 1 * 50000, SDS-PAGE | Lactiplantibacillus plantarum |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.20 | TanLpl | - |
Lactiplantibacillus plantarum |
| 3.1.1.20 | tannin acylhydrolase | - |
Lactiplantibacillus plantarum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.20 | 40 | - |
- |
Lactiplantibacillus plantarum |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.20 | 45 | 55 | the enzyme retains more than 50% activity between 25 and 45°C but the activity drops off markedly above 45°C | Lactiplantibacillus plantarum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.20 | 8 | - |
- |
Lactiplantibacillus plantarum |
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Release 2023.1 (January 2023)
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