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Literature summary extracted from

  • Miller, D.J.; Jerga, A.; Rock, C.O.; White, S.W.
    Analysis of the Staphylococcus aureus DgkB structure reveals a common catalytic mechanism for the soluble diacylglycerol kinases (2008), Structure, 16, 1036-1046.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.107 isoform DgkB, both as free enzyme and in complex with ADP, 2.4 and 2.3 A resolution, respectively. Enzyme is a tight homodimer, and each monomer comprises two domains with the catalytic center located within the interdomain cleft Staphylococcus aureus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.107 D124A residue involved in the Mg1-water network, no catalytic acitivity Staphylococcus aureus
2.7.1.107 D271A residue involved in the Mg1-water network, no catalytic acitivity Staphylococcus aureus
2.7.1.107 D68A no catalytic activity. Role of D68 in mediating the interaction of Mg2+ with the gamma-phosphate of ATP Staphylococcus aureus
2.7.1.107 E273A no catalytic activity Staphylococcus aureus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.107 Mg2+ structure reveals a Mg2+ site and an associated Asp-water-Mg2+ network Staphylococcus aureus
2.7.1.107 additional information neither Ca2+, Mn2+, nor Zn2+ is able to effectively replace Mg2+, although Ca2+ and Mn2+ support a few percent activity Staphylococcus aureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.107 37333
-
 x * 37333, calculated, x * 42000, SDS-PAGE Staphylococcus aureus
2.7.1.107 42000
-
 x * 37333, calculated, x * 42000, SDS-PAGE Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.107 Staphylococcus aureus Q6GFF9
-
-

Subunits

EC Number Subunits Comment Organism
2.7.1.107 ? x * 37333, calculated, x * 42000, SDS-PAGE Staphylococcus aureus