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Literature summary extracted from

  • Poli, A.; Salerno, A.; Laezza, G.; di Donato, P.; Dumontet, S.; Nicolaus, B.
    Heavy metal resistance of some thermophiles: potential use of alpha-amylase from Anoxybacillus amylolyticus as a microbial enzymatic bioassay (2009), Res. Microbiol., 160, 99-106.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.1 analysis the alpha-amylase from Anoxybacillus amylolyticus could be used in a sensitive method to detect very low concentrations of heavy metals Anoxybacillus amylolyticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.1 Cu2+ addition to growth medium in logarithmic phase, maximum inhibition of about 70-80% of enzyme expression at 0.47 mM. Addition to enzyme assay, 38.2% inhibition at 1.5 mM Anoxybacillus amylolyticus
3.2.1.1 Hg2+ addition to growth medium in logarithmic phase, 0.025 mM, no enzyme expression. Addition to enzyme assay, 69% inhibition at 0.5 mM Anoxybacillus amylolyticus
3.2.1.1 additional information Cr6+ addition to growth medium in logarithmic phase, maximum inhibition of about 70-80% at 1.15 mM; effect of a combination of different concentrations of two (Ni2+ + Zn2+, Cu2+ + Zn2+, Hg2+ + Ni2+, Hg2+ + Zn2+) or three metals (Hg2+ + Zn2+ + Ni2+) added to the logarithmic growth phase examined: in all cases an inhibitory effect higher than of the single metal is observed; Mn2+ addition to growth medium in logarithmic phase, 10% inhibition at about 1.1 mM of enzyme expression Anoxybacillus amylolyticus
3.2.1.1 Ni2+ addition to growth medium in logarithmic phase, maximum inhibition of enzyme expression of about 70-80% at 0.85 mM. Addition to enzyme assay, 11.5% inhibition at 1.5 mM Anoxybacillus amylolyticus
3.2.1.1 Zn2+ addition to growth medium in logarithmic phase, 0.03 mM, about 50% inhibition of enzyme expression, at 1 mM 100% inhibition. Addition to enzyme assay, 36.3% inhibition at 1.5 mM Anoxybacillus amylolyticus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Anoxybacillus amylolyticus
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 isolated from geothermal soil located on Mount Rittmann, Antartica
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3.2.1.1 Anoxybacillus amylolyticus MR3CT / ATCC BAA-872 / DSM 15939
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 isolated from geothermal soil located on Mount Rittmann, Antartica
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Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.1 partially purified from bacteria grown in the presence or in the absence of heavy metals Anoxybacillus amylolyticus